Efek Hepatotoksik Jus Pinang Muda (Areca catechu) pada Tikus

Authors

DOI:

https://doi.org/10.21776/ub.jkb.2018.030.02.3

Keywords:

Biji pinang, hepar, hepatotoksik, histopatologi, Jambi

Abstract


Hepatoksik akibat obat dan herbal merupakan penyebab kerusakan hepar yang menyebabkan morbiditas dan mortalitas. Jus pinang muda (Areca catechu) merupakan minuman herbal yang dikonsumsi oleh sebagian masyarakat di Jambi sebagai penambah stamina dengan takaran 5 biji pinang setara dengan 20gr/70kgBB atau 250mg/kgBB. Berdasarkan beberapa studi, pinang dosis 1000mg/kgBB dapat menurunkan kadar glukosa darah, sedangkan median lethal dose (LD 50) adalah >15.000mg/kgBB. Efek konsumsi jus pinang jangka panjang terhadap organ hepar belum diketahui. Penelitian ini merupakan uji pendahuluan untuk melihat efek pemberian jus pinang selama 45 hari terhadap organ hepar. Hewan coba adalah Rattus norvegicus galur Sprague Dawley, jantan, 2-3 bulan, berat 150-200 gram yang setelah diaklimatisasi dan dibagi secara random ke dalam 4 kelompok yaitu Kelompok I diberikan aquadest, kelompok II, III dan IV diberikan masing-masing jus pinang dosis 250mg/kgBB, 1.000mg/kgBB, dan 10.000mg/kgBB. Perlakuan diberikan sekali sehari selama 45 hari. Dilakukan pemeriksaan histopatologi menggunakan pewarnaan Hematoxillin Eosin. Hasil pemeriksaan histopatologi menunjukkan gambaran kerusakan tipe hepatoseluler yaitu perdarahan, dilatasi sinusoid, inflamasi portal, inflamasi lobular, lobular dissarray, nekrosis, interface hepatitis dan mikrosteatosis pada kelompok jus pinang dengan derajat kerusakan hepar pada dosis 10.000mg/kgBB lebih berat. Konsumsi jus pinang dosis 250-10.000mg/kgBB selama 45 hari dapat menimbulkan kerusakan hepar pada tikus.

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References

Kleiner DE, Chalasani NP, Lee WM, et al. Hepatic Histological Findings in Suspected Drug-Induced Liver Injury: Systematic Evaluation and Clinical Associations. Hepatology. 2014; 59(2): 661-670.

Licata A, Macaluso FS, and Craxi A. Herbal Hepatotoxicity: A Hidden Epidemic. Internal and Emergency Medicine. 2013; 8(1): 13-22.

Krishna YR, Mittal V, Grewal P, Fiel MI, and Schiano T. Acute Liver Failure Caused by “Fat Burners†and Dietary Supplements: A Case Report and Literature Review. Canadian Journal of Gastroenterology. 2011; 25(3): 157-160.

Teschke R, Genthner A, and Wolff A. Kava Hepatotoxicity: Comparison of Aqueous, Ethanolic, Acetonic Kava Extracts and Kava-Herbs Mixtures. Journal of Ethnopharmacology. 2009; 123(3): 378-384.

Li X, Qu C, He Q, et al. Acute Hepatitis Induced by A Chinese Herbal Product Qibao Meiran Wan: A Case Study. International Journal of Clinical and Experimental Medicine. 2015; 8(7): 11624-11627.

Ramachandran R and Kakar S. Histological Patterns in Drug-Induced Liver Disease. Journal of Clinical Pathology. 2009; 62(6): 481-492.

Amudhan MS and Begum H. Alpha-Glucosidase Inhibitory and Hypoglycemic Activities of Areca catechu Extract. Pharmacognosy Magazine. 2008; 4(15): 223-227.

Rahman AO and Utami RF. Hypoglycemic Effect of Raw Betel Nut. Proceeding Pertemuan Ilmiah Tahunan Ikatan Ahli Farmakologi Indonesia ke 16. Jakarta, Oktober 20-22, 2016: hal. 13-15.

Sari LM, Suyatna FD, Utami S, et al. Acute Oral Toxicity Study of Areca catechu Linn. Aqueous Extract in Sprague Dawley Rats. Asian Journal of Pharmaceutical and Clinical Research. 2014; 7(5): 20-22.

Jaiswal P, Kumar P, Singh VK, and Singh DK. Areca catechu L.: A Valuable Herbal Medicine Againts Different Health Problems. Research Journal of Medicinal Plant. 2011; 5(2): 145-152.

Prabhakar PK and Doble M. Interaction of Phytochemicals with Hypoglycemic Drugs on Glucose Uptake in L6 Myotubes. Phytomedicine. 2011; 18(4): 285-291.

Chou WW, Guh JY, Tsai JF, et al. Arecoline-Induced Growth Arrest and P21WAF1 Expression are Dependent on P53 in Rat Hepatocytes. Toxicology. 2008; 243(1-2): 1–10.

Yeh CY, Chen HM, Chang MC, et al. Cytotoxicity and Transformation of C3H10T1/2 Cells Induced by Areca Nut Components. Journal of the Formosan Medical Association. 2016; 115(2): 108-112.

Garg A, Chaturvedi P, and Gupta PC. A Review of the Systemic Adverse Effects of Areca Nut or Betel Nut. Indian Journal of Medical and Paediatric Oncology. 2014; 35(1): 3-9.

Frenzel C and Teschke R. Herbal Hepatotoxicity: Clinical Characteristics and Listing Compilation. International Journal of Molecular Sciences. 2016; 17(5): 1-38.

Stournaras E and Tziomalos K. Herbal Medicine-Related Hepatotoxicity. World Journal of Hepatology. 2015; 7(19): 2189-2193.

Calitz C, du Plessis L, Gouws C, et al. Herbal Hepatotoxicity: Current Status, Examples, and Challenges. Expert Opinion on Drug Metabolism & Toxicology. 2015; 11(10): 1551-1565.

Arsad SS, Esa NM, and Hamzah H. Histopathologic Changes in Liver and Kidney Tissues from Male Sprague Dawney Rats Treated with Rhaphidophora Decursiva (Roxb.) Schott Extract. Journal of Cytology Histology. 2014; 2014: 1-6.

Theise ND and Saxena N. Algorithmic Approach to Diagnosis of Liver Disorders. In: Odze RD and Goldblum JR (Eds). Surgical Pathology of the GI Tract, Liver, Billiary Tract, and Pancreas. 3rd edition. Philadelphia: Elsevier Saunders; 2015; pp. 1143-1169.

Fan CQ and Crawford JM. Sinusoidal Obstruction Syndrome (Hepatic Veno-Occlusive Disease). Journal of Clinical and Experimental Hepatology. 2014; 4(4): 332-346.

Amudhan S, Begum VH, and Hebbar KB. A Review on Phytochemical and Pharmacological Potential of Arecha Catechu L. Seed. International Journal of Pharmaceutical Sciences and Research. 2012; 3(11): 4151-4157.

Zhou J, Sun Q, Yang Z, and Zhang J. The Hepatotoxicity and Testicular Toxicity Induced by Arecoline in Mice and Protective Effects of Vitamins C and E. The Korean Journal of Physiology & Pharmacology. 2014; 18(2): 143-148.

Wei X, Zhang J, Niu J, Zhou X, Li J, and Li B. Evaluation of Arecoline Hydrobromide Toxicity After A 14-Day Repeated Oral Administration in Wistar Rats. PLoS ONE. 2015; 10(4): 1-23.

Nassir F, Rector S, Hammoud GM, and Ibdah JA. Pathogenesis and Prevention of Hepatic Steatosis. Gastroenterology & Hepatology. 2015; 11(3): 167-175.

Adediji JA, Adediji TA, and Eze GI. Effects of Short Term Oral Administration of Aqueous Extracts of Areca Nut (Areca catechu) on Liver Profile of Adult Wistar Rats. IOSR Journal of Dental and Medical Sciences. 2015; 14(10): 62-67.

Kullak-Ublick GA, Andrade RJ, Merz M, End P, et al. Drug-Induced Liver Injury: Recent Advances in Diagnosis and Risk Assessment. Gut. 2017; 66(6): 1154-1164

Published

2018-08-27

Issue

Section

Research Article