Anti-Inflammatory Effects of Ripe Areca Nut Ethyl Acetate Fraction in Wistar Rats with Knee Joints Osteoarthritis

Authors

  • Daffa Akrama Yuda Graduated Student of Medical Program Faculty of Medicine and Health Sciences Universitas Jambi Jambi
  • Ave Olivia Rahman Department of Pharmacology Faculty of Medicine and Health Sciences Universitas Jambi Jambi
  • Fathnur Sani Kasmadi Department of Pharmacy Faculty of Medicine and Health Sciences Universitas Jambi Jambi
  • Humaryanto Humaryanto Department of Orthopedic Surgery Faculty of Medicine and Health Sciences Universitas Jambi Jambi

DOI:

https://doi.org/10.21776/ub.jkb.2022.032.03.1

Keywords:

Areca nut, ethyl acetate fraction, histopathology, knee joints, osteoarthritis

Abstract

Osteoarthritis (OA) is the most common form of arthritis. Meloxicam are one of NSAIDs commonly used that have many side effects. Areca nut (Areca catechu L), which contains flavonoids, can provide anti-inflammatory effects. This study aimed to determine the potential anti-inflammatory effect of ethyl acetate fraction from ripe areca nut in OA rat model. Twenty five of adults, male Rattus novergicus strain Wistar were induced to OA with Monosodium Iodoacetate (MIA), then divided into five groups that each group were given ethyl acetate fraction of ripe areca nut doses of 15mg/kgBW, 30mg/kgBW, and 60mg/kgBW, meloxicam 1.35mg/kgBW and aquadest as negative control for 14 days. Diameter of the knee joint is measured by digital metric kaliper to assess joint edema. The Histopathological examination with Haematoxyllin-Eosin stain to assess degree of inflammation on synovial. Among the areca groups, dose 30mg/kgBW had knee joint diameter reduced better and lower synovitis score. In conclusion, ethyl acetate fraction of the ripe areca nut group has anti inflammation effect in OA model but not dose-dependent.

Downloads

Download data is not yet available.

References

Hawker GA. Osteoarthritis is a Serious Disease. Clinical and Experimental Rheumatology. 2019; 37(5): 3–6.

Adhiputra IKAI. Osteoartritis. [Responsi Kasus]. Universitas Udayana, Bali. 2017.

Mora JC, Przkora R, and Cruz-Almeida Y. Knee Osteoarthritis: Pathophysiology and Current Treatment Modalities. Journal of Pain Research. 2018; 11: 2189–2196.

Cooper C, Chapurlat R, Al-Daghri N, et al. Safety Of Oral Non ‑ Selective Non ‑ Steroidal Anti ‑ Inflammatory Drugs in Osteoarthritis: What Does The Literature Say? Drugs & Aging. 2019; 36(S1): 15–24.

Yuda DA. Efek Anti Inflamasi Ekstrak Biji Pinang Kuning (Areca Catechu L) Pada Sendi Lutut Tikus Putih (Rattus Novergicus) Model Osteoarthritis. [Skripsi]. Universitas Jambi, Jambi. 2019.

Takahashi I, Matsuzaki T, Kuroki H, and Hoso M. Induction of Osteoarthritis by Injecting Monosodium Iodoacetate Into the Patellofemoral Joint of an Experimental Rat Model. PLoS One. 2018; 13(4): 1-15.

Prayoga DGE, Nocianitri KA, and Puspawati NN. Identifikasi Senyawa Fitokimia dan Aktivitas Antioksidan Ekstrak Kasar Daun Pepe. Jurnal Ilmu dan Teknologi Pangan. 2019; 8(2): 111–121.

Ritna A, Anam S, and Khumaidi A. Identifikasi Senyawa Flavonoid pada Fraksi Etil Asetat Benalu Batu (Begonia Sp.) Asal Kabupaten Morowali Utara. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy). 2016; 2(2): 83–89.

Ramadhani MA, Hati AK, Lukitasari NF, and Jusman AH. Skrining Fitokimia dan Penetapan Kadar Flavonoid Total Serta Fenolik Total Ekstrak Daun Insulin (Tithonia Diversifolia) dengan Maserasi Menggunakan Pelarut Etanol 96 %. Indonesian Journal of Pharmacy and Nature Product. 2020; 3(1): 8–18.

Cahyanto HA. Aktivitas Antioksidan Ekstrak Etanol Biji Pinang (Areca Catechu, L). Majalah BIAM. 2018; 14(2): 70-73.

Lindawati NY and Ma'ruf SH. Penetapan Kadar Total Flavonoid Ekstrak Etanol Kacang Merah (Phaseolus Vulgaris L.) dengan Metode Kompleks Kolorimetri secara Spektrofotometri Visibel. Jurnal Ilmiah Manuntung. 2020; 6(1): 83–91.

Asmorowati H and Lindawati NY. Penetapan Kadar Flavonoid Total Alpukat (Persea americana Mill.) dengan Metode Spektrofotometri UV-Vis. Jurnal Ilmiah Farmasi. 2019; 15(2): 51–63.

Harnis ZE. Fraksinasi Dan Karakterisasi Serta Uji Antiinflamasi Ekstrak Etanol Daun Sambung Rambat (Mikania Cordata (Burm.F) B.L Rob) Terhadap Tikus Putih Jantan. [Thesis]. Universitas Sumatera Utara, Medan. 2019.

Wyatt LA, Moreton BJ, Mapp PI, et al. Histopathological Subgroups in Knee Osteoarthritis. Osteoarthritis And Cartilage. 2017; 25(1): 14–22.

Farry F, Ismiarto YD, Chaidir MR, and Ismono D. Histopathological Effects of Omega-3 in Reducing Cartilage Destruction Progression in Mice with Knee Joint Osteoarthritis. Majalah Kedokteran Bandung. 2020; 52(2): 107–112.

Pinandita T, Ismono D, Ismiarto YD, and Chaidir MR. Efek Pemberian Meloxicam yang Diberikan selama Fase Inflamasi terhadap Proses Penyembuhan Tulang Tikus Paska Open Reduction Internal Fixation K-Wire Dinilai secara Radiologis. Jurnal Sistem Kesehatan. 2018; 3(3): 135–141.

Maleki SJ, Crespo JF, and Cabanillas B. Anti-Inflammatory Effects of Flavonoids. Food Chemistry. 2019; 299: 1-11.

Lestari R, Djohari M, and Hasti S. Identifikasi dan Uji Aktivitas Daya Hambat Ekstrak Etanol Biji Pinang (Areca catechu L.) terhadap Isolat Bakteri Gusi. Jurnal Penelitian Farmasi Indonesia. 2019; 7(2): 61-69.

Downloads

Published

2023-02-27 — Updated on 2023-05-08

Versions

Issue

Vol. 32 No. 3 (2023)

Section

Research Article

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).