The Effect of Psychotria malayana Extract on Wound Healing in Male White Rats (Rattus Novergicus) Sprague Dawley Strain

Authors

  • Fairuz Fairuz Department of Anatomical Pathology Faculty of Medicine and Health Science Universitas Jambi Jambi
  • Khory Aurora Berty Department of Histology Faculty of Medicine and Health Science Universitas Jambi Jambi
  • Humaryanto Humaryanto Department of Surgery Faculty of Medicine and Health Science Universitas Jambi Jambi

DOI:

https://doi.org/10.21776/ub.jkb.2023.032.04.3

Keywords:

Histopathology, Psychotria malayana, wound healing, white rat (Rattus norvegicus)

Abstract

Normal wounds are a complex and dynamic process, but they have a predictable pattern, and untreated open lesions are potentially infectious. According to the CDC (Centers for Disease Control), the incidence of surgical site infection (SSI) in the United States in 2018 was 157,500, with an estimated mortality rate of 8,205. Psychotria malayana plant contains flavonoids, alkaloids, and tannins, which play a role in the inflammatory phase and proliferation of wound healing. Many pharmacology effects of alkaloid and flavonoid activities include anti-inflammatory properties. The aim of this study was to determine the effect of Psychotria malayana extract ointment on wound healing in male white rats (Rattus norvegicus) of the Sprague-Dowley strain. This study is a laboratory experiment using the randomized post-test control only group design. Subjects were 25 male white rats (Rattus norvegicus) of the Sprague-Dowley strain. The rats were divided into five groups. The treatment was carried out for 14 days, and the wounds were shaved under anesthesia, and the skin was taken for histopathological evaluation after it had been examined macroscopically. An independent test showed that there is no significant difference macroscopically between control groups and treatment groups with a p-value >0.05. The Mann Whitney test showed a significant comparison microscopically at the level of angiogenesis, fibroblasts, and collagen in group negative control with group I (10% Psychotria malayana extract ointment) with a P value 0.05%. The conclusion is that 10% Psychotria malayana extract ointment increases fibroblast levels, angiogenesis, and collagen production in wound healing.

Downloads

Download data is not yet available.

References

Rodrigues M, Kosaric N, Bonham C, and Gurtner GC. Wound Healing: A Cellular Perspective. Physiological Reviews. 2019; 99(1): 665-706.

Braiman-Wiksman L, Solomonik I, Spira R, and Tennenbaum T. Novel Insights Into Wound Healing Sequence of Events. Toxicologic Pathology. 2007; 35(6): 767–779.

Zabaglo M and Sharman T. Postoperative Wound Infection. Florida: StatPearls Publishing; 2022.

Pereira RF and Bártolo PJ. Traditional Therapies for Skin Wound Healing. Advances in Wound Care. 2016; 5(5): 208–229.

Petrovska BB. Historical Review of Medicinal Plants' Usage. Pharmacognosy Reviews. 2012; 6(11): 1-5.

Briggs M, de Kok R, Moat J, Whaley O and Williams J. Vegetation Mapping for Reforestation and Carbon Capture in the Harapan Rainforest. London: Defra; 2012.

Marusin S, Saefudin, and Chairul. Potensi Sifat Antioksidan pada 10 Jenis Ekstrak dari Famili Rubiaceae. Jurnal Biologi Indonesia. 2013; 9(1): 93–100.

Fazil M, Suci RN, Allfiah F, Alam DN, Angelia G, and Situmeang B. Analisis Senyawa Alkaloid dan Flavonoid dari Ekstrak Kitolod (Isotoma Longiflora) dan Uji Aktivitasnya terhadap Bakteri Penyebab Karies Gigi. Jurnal Itekima. 2017; 2(1): 73–83.

Noer S, Pratiwi RD, and Gresinta E. Penetapan Kadar Senyawa Fitokimia (Tanin, Saponin Dan Flavonoid) Sebagai Kuersetin Pada Ekstrak Daun Inggu (Ruta Angustifolia L.). Eksakta: Jurnal Ilmu-Ilmu MIPA. 2018; 18(1): 19–29.

Benchoula K, Khatib A, Quzwain FMC, et al. Optimization of Hyperglycemic Induction in Zebrafish and Evaluation of Its Blood Glucose Level and Metabolite Fingerprint Treated with Psychotria malayana Jack Leaf Extract. Molecules. 2019; 24(8): 1-22.

Nursanti, Novriyanti, and Wulan C. Ragam Jenis Tumbuhan Obat Potensial di Areal Hutan Kota Muhammad Sabki Kota Jambi. Media Konservasi. 2018; 23(2): 169–177.

Gal P, Kilik R, Mokry M, et al. Simple Method of Open Skin Wound Healing Model in Corticosteroid‑Treated and Diabetic Rats: Standardization of Semi‑Quantitative and Quantitative Histological Assessments. Veterinarni Medicina. 2008; 53(12): 652‑659.

Muthalib EM, Fatimawali, and Edy HJ. Formulasi Salep Ekstrak Etanol Daun Tapak Kuda (Ipomoea Pes-Caprae) dan Uji Efektivitasnya terhadap Luka Terbuka pada Punggung Kelinci. PHARMACON. 2013; 2(3): 79–82.

Pastar I, Stojadinovic O, Yin NC, et al. Epithelialization in Wound Healing: A Comprehensive Review. Advances in Wound Care. 2014; 3(7): 445–464.

Pratiwi A, Ratnawati R, and Kristianto H. Pengaruh Pemberian Ekstrak Kuncup Bunga Cengkeh (Syzygium aromaticum) terhadap Peningkatan Ketebalan Epitelisasi Luka Insisipada Tikus Putih (Rattus novergicus) Galur Wistar. Majalah Kesehatan FKUB. 2015; 2(3): 135–143.

Ferbam B, Wientarsih I, and Pontjo BP. Aktivitas Sediaan Salep Ektrak Batang Pohon Pisang Ambon (Musa Paradisiaca Var Sapientum) dalam Proses Persembuhan Luka pada Mencit (Mus musculus albinus). Majalah Obat Tradisional. 2010; 15(3): 121-137.

Putrianirma R, Triakoso N, Yunita MN, Yudaniayanti IS, Hamid IS, and Fikri F. Efektivitas Ekstrak Daun Afrika (Vernonia Amygdalina) secara Topikal untuk Reepitelisasi Penyembuhan Luka Insisi pada Tikus Putih (Rattus Novergicus). Jurnal Medik Veteriner. 2019; 2(1): 30-35.

Rahman AO and Humaryanto H. Efek Salep Ekstrak Pinang terhadap Level Fibroblast dan Kolagen pada Proses Penyembuhan Luka. Jambi Medical Journal. 2019; 7(1): 19–25.

Downloads

Published

2023-08-31

Issue

Vol. 32 No. 4 (2023)

Section

Research Article

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).