Pengaruh Artemisin dan Moringa terhadap Derajat Parasitemia, Kadar Malondialdehyde serta Histopatologi Otak Mencit Malaria

Authors

  • Tinny Endang Hernowati Brawijaya University
  • Loeki Enggar Fitri Brawijaya University
  • resti anggun pertiwi brawijaya University

DOI:

https://doi.org/10.21776/ub.jkb.2016.029.02.6

Abstract

Salah satu bentuk infeksi malaria berat adalah malaria serebral ditandai dengan respon inflamasi berlebih dan peningkatan jumlah pembuluh darah di jaringan otak sehingga menyebabkan terbentuknya radikal bebas secara berlebihan. Ekstrak daun Kelor (Moringa oleifera) berpotensi sebagai antioksidan dan antiinflamasi yang diharapkan dapat bekerja sebagai terapi adjuvant untuk kombinasi dengan artemisin. Penelitian ini bertujuan untuk menguji pengaruh kombinasi Artemisin dan ekstrak daun Moringa oleifera terhadap derajat parasitemia, kadar MDA dan gambaran histopatologi pada jaringan otak mencit yang diinfeksi P.berghei. Penelitian ini merupakan True Experimental Design. Sampel penelitian yang digunakan adalah mencit galur BALB/c. Bedasarkan hasil analisis Post Hoc pada hari ke-3 dan ke-7, terdapat perbedaan rerata derajat parasitemia, kadar MDA, dan jumlah pembuluh darah di jaringan otak yang bermakna antara kontrol positif dan semua perlakuan (p<0,05). Pada kelompok yang diterapi Artemisin, didapatkan derajat parasitemia yang lebih rendah dibandingkan dengan kontrol positif (p<0,05), namun lebih tinggi dibandingkan dengan kelompok kombinasi (p<0,05). Rerata kadar MDA turun signifikan pada hari ke 7 terutama pada kelompok kombinasi dosis tertinggi (p=0,000) dibandingkan dengan hari ke 3. Pada pengamatan jumlah pembuluh darah di jaringan otak mencit didapatkan bahwa pada hari ke 7 terjadi penurunan signifikan pada jumlah pembuluh darah di kelompok kombinasi dibandingkan kelompok Artemisin. Dapat disimpulkan kombinasi Artemisin dan ekstrak daun Moringa oleifera lebih baik menurunkan derajat parasitemia, kadar MDA dan jumlah pembuluh darah di jaringan otak mencit dibandingkan dengan terapi artemisin saja.Kata Kunci: Derajat parasitemia, histopatologi otak, malaria, Malondialdehyde

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References

World Health Organization. World Malaria Report: 2012. Switzerland: WHO Press: 2012; pp. 1-2, 39-44.

Idro R, Jenkins NE and Newton NE. Pathogenesis Clinical Features, and Neurological Outcome of Cerebral Malaria. The Lancet Neurology. 2005; 4(12): 827-840.

Jennings VM, Lal AA, and Hunters RL. Evidence for Multiple Pathologic and Protective Mechanisms of Murine Cerebral Malaria. Infection and Immunity. 1998; 66(12): 5972-5979

Brown GD. The Biosynthesis of Artemisinin (Qinghaosu) and the Phytochemistry of Artemisia Annua L. (Qinghao). Molecules. 2010; 15(11): 7603-7698

Kimbi HK, Ntoko M, Ntonifor NN, Lum E, Njunda AL, and Fon PN. Efficacy and Tolerability of Malartin and Sulphadoxine-Pyrimethamine Combination against Uncomplicated Falciparum Malaria in Dibanda, Southwest Cameroon. Journal of Tropical Medicine. 2012; 2012.

Percario S, Moreira DR, Gomes BAQ, et al. Oxidative Stress in Malaria. International Journal of Molecular Sciences. 2012; 13(12): 16346-16372.

Schmuck G, Roehrdanz E, Haynes RK, and Kahl R. Neurotoxic Mode of Action of Artemisinin. Antimicrobial Agents and Chemotherapy. 2002; 46(3); 821–827.

Rosenthal PJ. Artesunate for the Treatment of Severe Falciparum Malaria. The New England Journal of Medicine. 2008; 358: 1829-1836.

Winstanley P, Ward S, Snow R, and Breckenridge A. Therapy of Falciparum Malaria in Sub-Saharan Africa: from Molecule to Policy. Clinical Microbiology Reviews. 2004; 17(3): 612-637.

Woodrow CJ, Haynes RK and Krishna S. Artemisinins. Postgraduate Medical Journal. 2005; 81(952): 71-78.

Gupta S, Thapar MM, Wernsdorfer WH, and Bjorkman A. In vitro Interactions of Artemisinin with Atovaquone, Quinine and Mefloquine against Plasmodium falciparum. Antimicrobial Agents and Chemotherapy. 2002; 46(5): 1510-1515.

Rathod PK, McErlean T, and Lee PC. Variations in Frequencies of Drug Resistance in Plasmodium falciparum. Proceedings of the National Academy of Sciences of the United States of America. 1997; 94(17): 9389–9393.

Fitri LE, Iskandar A, Permatasari N, Gunawan JA, dan Indrawan K. Efek Kombinasi Artemisinin dan NAC, Menurunkan Kadar Otak dan Paru Mencit yang Diinfeksi N-acetylcysteine Malondialdehyde Plasmodium Berghei. Jurnal Kedokteran Brawijaya. 2008; 24(2): 73-78.

Pari L, Karamac M, Kosinska A, Rybarczyk A, and Amarowicz R. Antioxidant Activity of the Crude Extracts of Drumstick Tree (Moringa oleifera Lam.) and Sweet Broomweed (Scoparia Dulcis L.) Leaves. Polish Journal of Food and Nutrition Sciences. 2007; 57(2): 203-208.

Patel JP, Gami B, and Patel K. Evaluation of in Vitro Schizonticidal Properties of Acetone Extract of Some Indian Medicinal Plants. Advances in Biological Research. 2010; 4(5): 253-258.

Ugwu OPC, Ndowoo OFC, Joshua PE, et al. Anti-Malaria and Hematological Analyses of Ethanol Leaf Extract of Moringa oleifera on Malaria Infected Mice. International Journal of Pharmacy and Biological Science. 2013; 3(1): 360-371.

Sardjono TW dan Fitri LE. Malaria, Mekanisme Terjadinya Penyakit dan Pedoman Penanganannya. Malang: Laboratorium Parasitologi FKUB; 2007.

Anonymous. Bioxytech® MDA-586 Assay Kit. Spectrophotometric Assay for Malondialdehyde. (Online). http://www.oxisresearch.com/ product_details.html?prodid=21044.

Al-Dissi AN, Haines DB, Singh B, and Kidney BA. Immunohistochemical Expression of Vascular Endothelial Growth Factor and Vascular Endothelial Growth Factor Receptor-2 in Canine Simple Mammary Gland Adenocarcinomas. Canadian Veterinary Journal. 2010; 51(10): 1109-1114.

Krishnegowda G, Hajjar AM, Zhu J, et al. Induction of Proinflammatory Responses in Macrophages by the Glycosylphosphatidylinositols of Plasmodium falciparum: Cell Signaling Receptors, Glycosylphosphatidylinositol (GPI) Structural Requirement, and Regulation of GPI Activity. The Journal of Biological Chemistry. 2005; 280(9): 8606-8616.

Kleinert H, Scwarz PM, and Forstermann U. Regulation of the Expression of Inducible Nitric Oxide Synthase. The Biological Chemistry. 2003; 384(10-11): 1343-1364.

Shabir G, Anwar F, Sultana B, et al. Antioxidant and Antimicobial Attributes and Phenolics of Different Solvent Extracts from Leaves, Flowers and Bark of Gold Mohar [Delonix regia (Bojer ex Hook.) Raf.]. Molecules. 2011; 16(9): 7302-7319.

Malaquarnera L and Musumeci S. The Immune Response to Plasmodium falciparum Malaria. The Lancet Infectious Diseases. 2002; 2(8): 472-478.

Wresdiyati T. Imunohistochemical Study of Oxygen-Free Radical Scavenger-Copper, Zinc-Superoxide Dismutase (Cu,ZnSOD) in The Rats Liver Under Stress Condition. Biota. 2003; 8(3): 107-112.

Ogunro SP, Ogungbamigbe OT, Salawu AA, and Idogun SE. Erytrocyte Antioxidant Enymes and Lipid Peroxidation in Patient with Acute Attack of Plasmodium Falciparum. Oxidant and Antioxidant in Medical Science. 2012; 1(2): 111-118.

Raza A, Varshney SK, Shahid M, et al. Lipid Peroxidation in Cerebral Malaria and Role of Antioxidants. Journal of Pharmary. New Delhi. 2013; 3(1): 15-18.

Luqman S, Srivastava S, Kumar R, Maurya AK, and Chandra, D. Research Article: Experimental Assessment Moringa oleifera Leaf and Fruit for It's Antistress, Antioxidant, and Scavenging Potential Using In Vitro and In Vivo Assays. Evidence-Based Complementary and Alternative Medicine. Evidence-based Complementary and Alternative Medicine. 2012; 2012.

Odunlade A, Ashade AA, Akinosola JP, Akindele S, and Adewole, AA. Antioxidant Evaluation of Moringa oleifera Therapy Combination in Mice with Plasmodium berghei Malaria. 5th World Congress on Biotechnology, Valencia Conference Centre. Valencia, June 25-27, 2004: p. 3-7.

Deininger MH, Winkler S, Kremsner PG, Meyermann R, and Schluesener HJ. Angiogenic Proteins in Brains of Patients Who Died with Cerebral Malaria. Journal of Neuroimmunology. 2003; 142(1-2): 101-111.

Octo R. Pengaruh Sari Buah Merah (Pandanus conoideus Lam.). Terhadap Gambaran Histopatologi Otak Mencit Jantan Strain BALB/c Yang Diinokulasi Plasmodium berghei. [Tesis]. Universitas Maranatha, Bandung. 2009.

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Published

2016-08-29

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Research Article