Kombinasi Artesunat Injeksi dan Ekstrak Brotowali (Tinospora crispa (L) Miers) Menurunkan Derajat Parasitemia serta Meningkatkan Ekspresi HSP70 dan Endoglin pada Mencit C57BL/6J yang Diinfeksi Plasmodium berghei


  • Rahadi Swastomo Fakultas Kedokteran Hewan Universitas Brawijaya/ Program Studi Ilmu Biomedik Fakultas Kedokteran Universitas Brawijaya
  • Niniek Budiarti Fakultas Kedokteran Universitas Brawijaya/RSUD dr. Saiful Anwar, Malang
  • Loeki Enggar Fitri Fakultas Kedokteran Universitas Brawijaya Malang
  • Sri Poeranto Fakultas Kedokteran Universitas Brawijaya Malang




Artesunat, brotowali, endoglin, HSP70, malaria, Plasmodium berghei


Penemuan kombinasi terapi baru untuk malaria dianggap penting karena kemampuan resistensi dari Plasmodium terhadap pengobatan terdahulu. Sebanyak 18 mencit dibagi ke dalam kelompok kontrol negatif, kontrol positif, kelompok artesunat, serta kelompok kombinasi artesunat dengan brotowali (dosis 50 mg, 60 mg dan 70 mg). Selain kontrol negatif, semua mencit diinfeksi Plasmodium berghei. Selanjutnya diamati derajat parasitemianya sampai hari ke-7, kemudian dilakukan pemeriksaaan imunohistokimia guna mengetahui ekspresi HSP70 dan endoglin pada otak mencit. Kombinasi artesunat injeksi dan ekstrak brotowali terbukti dapat menurunkan derajat parasitemia lebih baik dari pemberian terapi tunggal artesunat dan meningkatkan ekspresi HSP70 dan endoglin. Kesimpulannya, kombinasi terapi malaria menggunakan artesunat dan brotowali terbukti berpotensi sebagai terapi kombinasi yang efektif terhadap malaria karena mampu menurunkan derajat parasitemia sampai 0% dan meningkatkan ekspresi HSP70 dan endoglin pada otak mencit C57BL/6J secara signifikan. 


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Author Biographies

Rahadi Swastomo, Fakultas Kedokteran Hewan Universitas Brawijaya/ Program Studi Ilmu Biomedik Fakultas Kedokteran Universitas Brawijaya

Bagian parasitologi di Fakultas Kedokteran Hewan UB

mahasiswa Pascasarjana di Program Studi Biomedik FKUB

Niniek Budiarti, Fakultas Kedokteran Universitas Brawijaya/RSUD dr. Saiful Anwar, Malang

Divisi Tropikal Infeksi, Bagian Penyakit Dalam, Fakultas Kedokteran Universitas Brawijaya/RSUD dr. Saiful Anwar, Malang

Loeki Enggar Fitri, Fakultas Kedokteran Universitas Brawijaya Malang

Departemen Parasitologi, Fakultas Kedokteran Universitas Brawijaya, Malang

Sri Poeranto, Fakultas Kedokteran Universitas Brawijaya Malang

Departemen Parasitologi, Fakultas Kedokteran Universitas Brawijaya, Malang


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