High Level of Plasma Tumour Necrosis Factor-α (TNF- α) in Pregnant Mice Infected with Plasmodium berghei is Strongly Related to Low Level of Hemoglobin but not Related to Fetal Low Weight
DOI:
https://doi.org/10.21776/ub.jkb.2015.028.04.3Abstract
Malaria infection in pregnancy may increase the morbidity and mortality for both mother and fetus. In pregnant women, it can lead to severe anemia, cerebral malaria, pulmonary edema, renal failure and even death, while in the fetus it can cause abortion, premature birth, low birth weight, and fetal death. Elevated levels of tumor necrosis factor-α (TNF-α ) are associated with low birth weight and anemia in pregnant women. This study was conducted to measure the levels of TNF-α in plasma and hemoglobin levels as well as fetal weight to determine the relationship among them in P. berghei infected pregnant mice and normal pregnant mice. Seventeen BALB/c mice used in this study were divided into two groups, those were the study group (9 pregnant mice infected with P. berghei) and control group (8 pregnant mice not infected with P. berghei). Levels of TNF-α were measured using Enzyme Linked Immunosorbent assay (R&D Systems, catalog A00B MT). Hemoglobin levels were determined using flowcytometri, whereas fetal weights were measured with Mettler analytical balance AE 50. T-test statistical analysis showed that the levels of plasma TNF-α in study group were higher than control group (p=0,000). Hemoglobin levels in the study group  were lower than control group (p=0,025). Fetal weights were also lower in fetuses of infected mice than fetuses of uninfected mice (p=0,002). Pearson correlation test showed that increasing plasma levels of TNF-α in infected P. berghei pregnant mice were related with the decreasing levels of Hb, (p=0,020; r=-0,748).  However plasma levels of TNF-α were not associated with the incidence of fetal low weight (p=0,380, and p=0,365). It can be concluded that the increasing levels of TNF-α is associated with decreasing levels of hemoglobin (Hb), but not associated with fetal low weight.Keywords:  Birth weight, hemoglobin, Plasmodium berghei, Tumour Necrosis Factor-α (TNF- α)
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Achidi EA, Kuoh AJ, Minang JT, et al. Malaria Infection in Pregnancy and Its Effects on Hemoglobin Levels in Women from a Malaria Endemic Area of Feko Division South West Province, Cameroon. Journal of Obstetrics and Gynaecology. 2005; 25(3): 235-240.
Erhabor O, Adias TC, and Hart ML. Effects of Falciparum Malaria on the Indices of Anemia among Pregnant Women in the Niger De ta of Nigeria. Journal of Clinical Medicine and Research. 2010; 2(3): 35-41.
Raghupathy, R. (1997). Th1-Type Immunity Is Incompatible With Successful Pregnancy. Immunology Today. 1997; 18(10): 478–482.
Suparman E dan Suryawan A. Malaria pada Kehamilan. Jurnal Kedokteran Maranatha. 2004; 4(1): 21-39.
Pearson RD. Parasites, Pregnancy, Prolactin and Pandemics? Trends Parasitolog. 2005; 21(12): 555–556.
Desai M, Kuilo FO, Nosten F, et al. Epidemiology and Burden of Malaria in Pregnancy. The Lancet Infectious Diseases. 2007; 7(2): 93–104.
Anya SE. Seasonal Variation in the Risk and Causes of Maternal Death in the Gambia Malaria Appears to be an Important Factor. The American Journal of Tropical Medicine and Hygiene. 2004; 70(5): 510-513.
Guyatt HL and Snow RW. The Epidemiology and Burden Plasmodium falciparum Related Anemi among Pregnant Woman in Sub Sahara Africa. The American Journal of Tropical Medicine and Hygiene. 2001; 64(1-2): 36-44.
Saba N, Sultana A, and Mahsud I. Outcome and Complications of Malaria in Pregnancy. Goma Journal of Medical Sciences. 2008; 6(2): 98-101.
Poespoprodjo JR, Fobia W, Kenangalem E, et al. Adverse Pregnancy Outcome in an Area Where Multidrug-Resistant Plasmodium vivax and Plasmodium falciparum Infections are Endemic. Clinical Infections Diseases. 2008; 46(9): 1374-1381.
Luxemburger C, McGready R, Kham A, et al. Effects of Malaria during Pregnancy on Infant Mortality in an Area of Low Malaria Transmission. American Journal of Epidemiology. 2001; 154(5): 459-465.
Rogerson SJ, Brown HC, Pollina E, et al. Placental Tumor Necrosis Factor Alpha but Not Gamma Interferon is Associated with Placental Malaria and Low Birth Weight in Malawian Women. Infection and Immunity. 2003; 71(1): 267-270.
Uneke CJ. Impact of Placental Plasmodium falsiparum Malaria Pregnancy and Perinatal Outcome in Sub Saharan Africa. Yale Journal of Biology and Medicine. 2008; 81(1): 1-7.
Kwak-Kim JY, Gilman-Sachs A, and Kim CE. T Helper 1 and 2 Immune Responses in Relationship to Pregnancy, Nonpregnancy, Recurrent Spontaneous Abortions and Infertility of Repeated Implantation Failures. Chemical Immunology and Allergy. 2005; 88: 64–79.
Fried M, Muga RO, Misore AO, and Duffy PE. Malaria Elicits Type 1 Cytokines in the Human PlacentaI IFN and TNF-α Associated with Pregnancy Outcomes. The Journal of Immunology. 1998; 160(5): 2523-2530.
Moormann AM, Sullivan AD, Rochford RA, et al. Malaria And Pregnancy: Placental Cytokine Expression And Its Relationship To Intrauterine Growth Retardation. The Journal of Infection Diseases. 1999; 180(6): 1987–1993.
Suguitan ALJr, Cadigan TJ, Nguyen TA, et al. Malaria-Associated Cytokine Changes in the Placenta of Women with Pre-Term Deliveries in Yaounde, Cameroon. The American Journal of Tropical Medicine and Hygiene. 2003; 69(6): 574–81.
Sardjono TW. Pengaruh Infeksi Toxoplasma pada Hasil Kehamilan Melalui Interferon-Gamma (IFN-Γ), Aktivitas Caspase 3 dan Apoptosis Sel-Sel Plasenta. [Tesis]. Universitas Airlangga, Surabaya. 2007.
Abbas AK and Lichtman AH. Cellular and Molecular Immunology. 6th edition. Philadelphia; Elseveir Saunder: 2011.
Clark IA, Budd AC, Alleva LM, and Cowden WB. Human Malarial Disease: A Consequence of Inflammatory Cytokine Release. Malaria Journal. 2006; 5(85): 1-32.
Ouma P, van Eijk AM, Hamel MJ, et al. Malaria and Anemia among Pregnant Women at First Antenatal Clinic Visit in Kisumu, Western Kenya. Tropical Medicine and International Health. 2007; 12(12): 1515-1523.
Ouédraogo S, Koura GK, Bodeau-Livinec F, Accrombessi MM, Massougbodji A, and Cot M. Maternal Anemia in Pregnancy: Assesing the Effect of Routine Preventive Measures in a Malaria-Endemic Area. The American Journal of Tropical Medicine and Hygiene. 2013; 88(2): 292-300.
Crawley J. Reducing the Burden of Anemia in Infants and Young Children in Malaria Endemic Countries of Africa: From Evidence to Action. The American Journal of Tropical Medicine and Hygiene. 2004; 71(2): 25-34.
Price RN, Simpson JA, Nosten F, et al. Factors Contributing to Anemia after Uncomplicated Falciparum Malaria. The American Journal of Tropical Medicine and Hygiene. 2001; 65(5): 614-622.
Lamikanra AA, Brown D, Potocnik A, Casals-Pascual C, Langhorne J, and Roberts DJ. Malaria Anemia; Of Mice and Man. Blood. 2007; 110(1): 18-28.
Mohan K, Dubey ML, Ganguly NK, and Mahajan RC. Plasmodium Falciparum: Role of Activated Blood Monocytes in Erythrocyte Membrane Damage and Red Cell Loss During Malaria. Experimental Parasitology. 1995; 80: 54-63.
Singh N, Shukla MM, and Sharma VP. Epidemiology of Malaria in Pregnancy in Central India. Bulletin of the World Health Organization. 1999; 77(7): 567-572.
Abrams ET, Kwiek JJ, Mwapasa V, et al. Malaria during Pregnancy and Foetal Haemological Status in Blantyre Malawi. Malaria Journal. 2005; 4(39): 1-8.
Miller KL, Silverman PH, Kullgren B, and Mahlmann LJ. Tumor Necrosis Factor Alpha and the Anemia Associated with Murine Malaria. Infection and Immunity. 1989; 57(5): 1542–1546.
Yap GS and Stevenson MM. Inhibition of In Vitro Erythropoiesis by Soluble Mediators in Plasmodium chabaudi AS Malaria: Lack of a Major Role for Interleukin 1, Tumor Necrosis Factor Alpha, and Gamma Interferon. Infection and Immunity. 1994; 62(2): 357- 362.
Dufour C, Corcione A, and Svahn J. TNF-Alpha Inhibits All Stages of Erythropoiesis, TNF Alpha. Blood. 2003; 102(6): 2053-2059.
Felli N, Pedini F, Zeuner A, et al. Multiple Members of the TNF Superfamily Contribute to IFN-Gamma-Mediated Inhibition of Erythropoiesis. The Journal of Immunology. 2005; 175(3): 1464-1472.
Rogerson SJ, Mwapasa V, and Meshnick SR. Malaria in Pregnancy: Linking Immunity and Pathogenesis to Prevention. The American Journal of Tropical Medicine and Hygiene. 2007; 77(6): 14-22.
Ayoola OO, Whatmore A, Balogun WO, Jarrett OO, Cruichshank JK, and Clayton PE. Maternal Malaria Status and Metabolic Profiles in Pregnancy and in Cord Blood: Relationships with Birth Size in Nigerian Infants. Malaria Journal. 2011; 11(75): 1-9.
Rodriguez-Morales AJ, Sanchez E, Vargas M, et al. Short Report: Pregnancy Outcomes Associated with Plasmodium Vivax Malaria in Northeastern Venezuela. The American Journal of Tropical Medicine and Hygiene. 2006; 74(5): 755-757.
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