Holothuria scabra Memperbaiki Fibrosis Hepar pada Tikus yang Diinduksi Karbon Tetraklorida

Authors

  • Novrita Padauleng Fakultas Kedokteran, Universitas Mataram, Jl. Pendidikan No. 37, Mataram,83125, Nusa Tenggara Barat. No. Telp.(0370)640874, FAX (0370)641717, HP 082147133498
  • Nurhidayati Nurhidayati Fakultas Kedokteran, Universitas Mataram, Jl. Pendidikan No. 37, Mataram,83125, Nusa Tenggara Barat. No. Telp.(0370)640874, FAX (0370)641717, HP 087865117385

DOI:

https://doi.org/10.21776/ub.jkb.2016.029.02.7

Abstract

Perkembangan agen antifibrotik belakangan ini mencakup berbagai mekanisme, diantaranya melalui jalur antioksidan. Salah satu bahan alam yang diketahui memiliki aktivitas antioksidan adalah Teripang pasir (Holothuria scabra). Tujuan penelitian ini adalah menganalisa aktivitas antifibrotik Teripang pasir in vivo menggunakan tikus fibrosis hepar yang diinduksi CCl4. Dua puluh delapan tikus Wistar jantan dibagi menjadi 4 kelompok penelitian, yang terdiri dari kelompok kontrol vehicle (injeksi minyak zaitun intraperitoneal), kelompok kontrol induksi CCl4 (injeksi CCl4 dalam minyak zaitun intraperitoneal), serta dua kelompok perlakuan (tikus diinduksi CCl4 dan diberikan Teripang pasir 75 dan 100mg/200gBB). Fibrosis hepar diinduksi dengan injeksi CCl4 10% dalam minyak zaitun serial selama 5 minggu, diikuti 4 minggu pemberian Teripang pasir peroral (75 & 100mg/200gBB) sekali sehari, sejak minggu ke-5. Fibrosis hepar dianalisa menggunakan sistem scoring METAVIR pada jaringan dengan pewarnaan Mallory. Hasil penelitian menunjukkan bahwa derajat fibrosis hepar pada kelompok kontrol induksi CCl4 berbeda signifikan dengan kelompok perlakuan yang diinduksi CCl4 dan diberikan Teripang pasir (p=0,000), namun perbedaan antara kelompok perlakuan yang diberikan Teripang pasir 75 dan 100mg/200gBB tidak signifikan (p=0,081). Sebagai kesimpulan, pemberian ekstrak Teripang pasir dapat memperbaiki fibrosis hepar yang diinduksi CCl4.
Kata Kunci: CCl4, fibrosis hepar, teripang pasir (Holothuria scabra)

Downloads

Download data is not yet available.

Author Biographies

Novrita Padauleng, Fakultas Kedokteran, Universitas Mataram, Jl. Pendidikan No. 37, Mataram,83125, Nusa Tenggara Barat. No. Telp.(0370)640874, FAX (0370)641717, HP 082147133498

Bagian Histologi

Nurhidayati Nurhidayati, Fakultas Kedokteran, Universitas Mataram, Jl. Pendidikan No. 37, Mataram,83125, Nusa Tenggara Barat. No. Telp.(0370)640874, FAX (0370)641717, HP 087865117385

Bagian Farmakologi

References

Butt AA, Yan P, Lo RV, et al. Liver Fibrosis Progression in Hepatitis C Virus Infection after Seroconversion. Journal of the American Medical Association Internal Medicine. 2015; 175(2): 178-185.

Wong GL, Chan HL, Yu Z, Chan HY, Tse CH, and Wong VW. Liver Fibrosis Progression in Chronic Hepatitis B Patients Positive for Hepatitis B eAntigen: A Prospective Cohort Study with Paired Transient Elastography Examination. Journal of Gastroenterology and Hepatology. 2013; 28(11): 1762-1769.

Ponomarenko Y, Leo MA, Kroll W, and Lieber CS. Effects of Alcohol Consumption on Eight Circulating Markers of Liver Fibrosis. Alcohol and Alcoholism. 2002; 37(3): 252-255.

Pandit A, Sachdeva T, and Bafna P. Drug-Induced Hepatotoxicity : A Review. Journal of Applied Pharmaceutical Science. 2012; 2(5): 233-243.

Angulo P, Keach JC, Batts KP, and Lindor KD. Independent Predictors of Liver Fibrosis in Patients with Nonalcoholic Steatohepatitis. Hepatology. 1999; 30(6): 1356-1362.

Iredale JP, Benyon RC, Pickering J, et al. Hepatic Stellate Cell Apoptosis and Reduced Hepatic Expression of Metalloproteinase Inhibitors. The Journal of Clinical Investigation. 1998; 102(3): 538-549.

Nikolaidis N, Kountouras J, Giouleme O, et al. Colchicine Treatment of Liver Fibrosis. Hepato-gastroenterology. 2006; 53(68): 281-285.

Ezhilarasan D, Sokal E, Karthikeyan S, and Najimi M. Plant Derived Antioxidants and Antifibrotic Drugs: Past, Present and Future. Journal of Coastal Life Medicine. 2014; 2(9): 738-745.

Bordbar S, Anwar F, and Saari N. High-Value Components and Bioactives from Sea Cucumbers for Functional Foods- A Review. Marine Drugs. 2011; 9(10): 1761-1805.

Okorie OE, Ko SH, Go S, et al. Preliminary Study of the Optimum Dietary Ascorbic Acid Level in Sea Cucumber, Apostichopus japonicus (Selenka). Journal of the World Aquaculture Society. 2008; 39(6): 758-765.

Mamelona J, Pelletier E, Girard-Lalancete K, Legault J, Karboune S, and Kermasha S. Quantification of Phenolic Contents and Antioxidant Capacity of Atlantic Sea Cucumber, Cucumaria frondosa. Food Chemistry. 2007; 104(3): 1040-1047.

Althunibat OY, Hashim RB, Taher M, Daud JM, Ikeda MA, and Zali B. In Vitro Antioxidant and Antiproliferative Activities of Three Malaysian Sea Cucumber Species. European Journal of Scientific Research. 2009; 37(3): 376-387.

Ha BJ and Lee JY. The Effect of Chondroitin Sulfate against CCl4-Induced Hepatotoxicity. Biological and Pharmaceutical Bulletin. 2003; 26(5): 622-626.

Fredalina BD, Ridwan BH, Abidin AA, et al. Fatty Acid Compositions in Local Sea Cucumber, Stichopus chloronotus, for Wound Healing. General Pharmacology: The Vascular System. 1999; 33(4): 337-340.

Wang J, Wang Y, Tang Q, et al. Antioxidant Activities of Low Molecular Weight Gelatin Hydrolysate Isolated from the Sea Cucumber Stichopus japonicus. Journal of Ocean University of China. 2010; 9(1): 94-98.

Nurhidayati. Efek Protektif Teripang Pasir (Holothuria scabra) terhadap Hepatotoksistas yang Diinduksi Karbon Tetraklorida (CCl4). [Tesis]. Universitas Airlangga, Surabaya. 2009.

Jang JH, Kang KJ, Kim YH, Kang YN and Lee IS. Reevaluation of Experimental Model of Hepatic Fibrosis Induced by Hepatotoxic Drugs: An Easy, Applicable, and Reproducible Model. Transplantation Proceedings. 2008; 40(8): 2700-2703.

Bedossa P. Intraobserver & Interobserver Variations in Liver Biopsy Interpretation in Patients with Chronic Hepatitis C. Hepatology. 1994; 20(1): 15-20.

Liedtke C, Luedde T, Sauerbruch T, et al. Experimental Liver Fibrosis Research: Update on Animal Models, Legal Issues and Translational Aspects. Fibrogenesis Tissue Repair. 2013; 6(1): 1-25.

Domitrovic R, Jakovac H, Tomac J, and Sain I. Liver Fibrosis in Mice Induced by Carbon Tetrachloride and its Reversion by Luteolin. Toxicology and Applied Pharmacology. 2009; 241(3): 311-321.

Lee GP, Jeong WI, Jeong DH, Do SH, Kim TH, and Jeong KS. Diagnostic Evaluation of Carbon Tetrachloride-induced Rat Hepatic Cirrhosis Model. Anticancer Research. 2005; 25(2A): 1029-1038.

Mogler C, Wieland M, Konig C, et al. Hepatic Stellate Cell-Expressed Endosialin Balances Fibrogenesis and Hepatocyte Proliferation during Liver Damage. EMBO Molecular Medicine. 2015; 7(3): 332-338.

Wafa JA, Adi TK, Hanapi A, dan Fasya AG Penentuan Kapasitas Antioksidan dan Kandungan Fenolik Total Ekstrak Kasar Teripang pasir (Holothuria scabra) dari Pantai Kenjeran Surabaya. ALCHEMY. 2014; 3(1): 76-83.

Bataller R and Brenner DA. Liver Fibrosis. The journal of Clinical Investigation. 2005; 115(2): 209-218.

Casini A, Ceni E, Salzano R, et al. Neutrophil-Derived Superoxide Anion Induces Lipid Peroxidation and Stimulates Collagen Synthesis in Human Hepatic Stellate Cells: Role of Nitric Oxide. Hepatology. 1997; 25(2): 361-367.

Downloads

Published

2016-08-29

Issue

Vol 29, No. 2 (2016)

Section

Research Article

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).