Combination of Vitamin C and E Improves Spermatogenesis of White Male Rat Model of Paradoxical Sleep Deprivation Stress

Authors

  • Fitranto Arjadi
  • Mustofa Mustofa
  • Yudhi Wibowo
  • Nur Signa Aini Gumilas
  • Dzicky Rifqi Fuadi

DOI:

https://doi.org/10.21776/ub.jkb.2022.032.01.2

Keywords:

Paradoxical sleep deprivation, spermatogenesis, vitamin C, vitamin E

Abstract

Paradoxical Sleep Deprivation (PSD) elevates glucocorticoid and Reactive Oxygen Species (ROS) levels that cause oxidative stress, trigger spermatogenic cells damage, and reduce the number of sertoli cells. Vitamin C and E are antioxidants that could prevent spermatogenesis damage by preventing free radical formation. The study aimed to determine the effect of single and combined doses of vitamin C and E in improving spermatogenesis of white male rats (Rattus norvegicus) model of PSD. This is experimental research with post-test only and control group design on 28 white male rats distributed into four groups, i.e. group I (control, PSD), group II (PSD+vitamin C), group III (PSD+vitamin E), and group IV (PSD+a combination of vitamin C and E). Testicular preparations were stained using Hematoxylin-Eosin staining, quantitative scores of spermatogenic cells were measured using the Johnsen method, and the number of sertoli cells was counted in 10 seminiferous tubules in each of the three testicular sections. Group I has the lowest mean of Johnsen score (5.27±0.28), and group IV has the highest mean score (8.95±0.62), while the Mann-Whitney test showed a significant difference (p<0.05) between group II, III and IV compared to group I (control group). Mean sertoli cells number of group I is the lowest (10.66±1.04), and group IV has the highest sertoli cells number. The post-hoc LSD test showed a significant difference (p<0.05) between group IV and the other groups. Thus, the combination of vitamins C and E improves Johnsen score and sertoli cells number of male rats (Rattus norvegicus) experiencing PSD.

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References

Hampl R and Stárka L. Glucocorticoids Affect Male Testicular Steroidogenesis. Physiological Reserch. 2020; 69(Suppl 2): S205-S210,

Venâncio DP, Andersen ML, Vilamaior PSL, Sleep Deprivation Alters Rat Ventral Prostate Morphology, Leading to Glandular Atrophy: A Microscopic Study Contrasted with the Hormonal Assays. Journal Biomedicine and Biotechnology. 2012; 2012: 1-7

Mahat RK, Kumar S, Arora M, Bhale DV, Mehta R, and Batra J. Role of Oxidative Stress and Antioxidants in Male Infertility. International Journal of Health Science Research. 2015; 5(3); 324-333.

Sharma A, Singh AK, and Singh SK. Increasing Prevalence of Male Infertility and Stress Factors. International Journal of Clinical Cases and Investigations. 2011; 3(2): 17-24

Walczak-Jedrzejowska R, Wolski JK, and Slowikowska-Hilczer J. The Role of Oxidative Stress and Antioxidants in Male Fertility. Central Europe Journal of Urology. 2013; 66(1): 60-67.

Saxena S and Singh SP. Efficacy of Vitamin E and Vitamin C Against Silica Induced Toxicity on Male Reproductive Organs of Albino Rats. Journal Applied and Natural Science. 2012; 4(1): 127–131.

Keshavars M, Takhsid MA,Tavasoli A, Jahromi HK, Meshkibaf MF, and Heydari Y. The Protective Effects of Vitamin E and C against Oxidative Stress Induced by Sulfasalazine in the Testis of Male Adult Rats. Journal of Advanced Biomedical Sciences. 2012; 1(4): 206-215.

Muthu K and Krishnamoorthy P. Effect of Vitamin C and Vitamin E on Mercuric Chloride -Induced Reproductive Toxicity in Male Rats. Biochemia Pharmacology Open Access. 2012; 1(7): 1–5.

Kalsum U, Ilyas S, and Hutahean S. Pengaruh Pemberian Vitamin C Dan E terhadap Gambaran Histologis Testis Mencit (Mus musculus L.) yang Dipajankan Monosium Glutamat (MSG). Saintia Biologi. 2013; 1 (3): 7–12.

Arjadi F, Soejono SK, and Pangestu M. Paradoxical Sleep Deprivation Decreases Serum Testosterone and Leydig Cells in Male Rats. Universa Medicina. 2014; 33(1): 27–35.

Kusumawati D. Bersahabat dengan Hewan Coba. 2nd edition. Yogyakarta: Gadjah Mada University Press; 2016: p. 5–136.

Oh MM, Kim JW, Jin MH, Kim JJ, and Moon DG. Influence of Paradoxical Sleep Deprivation and Sleep Recovery on Testosterone Level in Rats of Different Ages. Asian Journal of Andrology. 2012; 14(4): 330-334.

Hazra R, Upton D, Jimenez M, Desai R, Handelsman DJ, and Allan CM. In Vivo Actions of the Sertoli Cell Glucocorticoid Receptor. Endocrinology. 2014; 155(3): 1120–1130.

Angulo C, Maldonado R, Pulgar E, et al. Vitamin C and Oxidative Stress in the Seminiferous Epithelium. Biological Research. 2011; 44(2): 169-180.

Tang VM. Glucocorticoid Effects on Oxidative Stress and Mitochondrial Dysfunction. [Thesis]. The University of British Columbia, Vancouver. 2012.

Mathur PP, Huang L, Kashou A, Vaithinathan S, and Agarwal A. Environmental Toxicants and Testicular Apoptosis. The Open Reproductive Science Journal. 2011; 3: 114–124.

Mahdavi M and Mozdarani H. Protective Effects Of Famotidine and Vitamin C Against Radiation Induced Cellular Damage in Mouse Spermatogenesis Process. Iran Journal of Radiation research. 2011; 8(4): 223-230.

Hamid NAA, Hasrul MA, Ruzanna RJ, et al. Effect of Vitamin E (Tri E®) on Antioxidant Enzymes and DNA Damage in Rats Following Eight Weeks Exercise. Nutrition Journal. 2011;10(1): 1-7

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Published

2022-02-28 — Updated on 2022-04-11

Versions

Issue

Vol. 32 No. 1 (2022)

Section

Research Article

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