Combination of Vitamin C and E Improves Spermatogenesis of White Male Rat Model of Paradoxical Sleep Deprivation Stress
DOI:
https://doi.org/10.21776/ub.jkb.2022.032.01.2Abstract
Paradoxical Sleep Deprivation (PSD) elevates glucocorticoid and Reactive Oxygen Species (ROS) levels that cause oxidative stress, trigger spermatogenic cells damage, and reduce the number of Sertoli cells. Vitamin C and E are antioxidants that could prevent spermatogenesis damage by preventing free radical formation. The study aimed to determine the effect of single and combined doses of vitamin C and E in improving spermatogenesis of white male rats model of PSD. This is experimental research with post-test only and control group design on 28 white male rats distributed into four groups, i.e. group I (control, PSD), group II (PSD + vitamin C), group III (PSD + vitamin E), and group IV (PSD + a combination of vitamin C and E). Testicular preparations were stained using Hematoxylin-Eosin, scores of spermatogenic cells were measured using the Johnsen method, and the number of Sertoli cells was counted in 10 seminiferous tubules in each of the three testicular sections. Group I has the lowest mean of spermatogenic score (5,27±0,28), and group IV has the highest mean score (8,95± 0,62), while the Mann-Whitney test showed a significant difference (p<0,05) between group II, III and IV compared to group I (control group). Mean Sertoli cells number of group I is the lowest (10.66±1.04), and group IV has the highest Sertoli cells number. The post-hoc LSD test showed a significant difference (p<0,05) between group IV and the other groups. Thus, the combination of vitamins C and E improves the spermatogenic score of male rats experiencing PSD.
Downloads
Published
Versions
- 2022-04-11 (3)
- 2022-02-28 (2)
- 2022-02-28 (1)
Issue
Section
License
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
