User
Information for Reviewer
Index

JKB telah terindex di Crossref JKB telah terindex di Google Scholar JKB telah terindex di SINTA Ristekdikti JKB telah terindex di Portal Garuda

Notifications
Journal Content

Browse
Visitor Counter

 

Efek Polisakarida Mesona Chinensis pada Produksi Sitokin dan Molekul Kostimulator pada Sel Makrofag RAW264.7

Andri Praja S, Tzou Chi Huang, Sumarno Sumarno
  Jurnal Kedokteran Brawijaya, Vol 29, No 1 (2016),  pp.14-20  

Abstract


Polisakarida yang berasal dari tumbuhan telah menjadi pusat perhatian pada keilmuan biomedik karena memiliki berbagai kemampuan biologis dan toksisitasnya yang rendah. Pada penelitian sebelumnnya, polisakarida Mesona chinensis (MCP) yang didapat dari ekstrak tanaman cincau atau grass jelly telah menunjukkan kemampuannya sebagai imunostimulator dan anti kanker, akan tetapi bagaimana mekanismenya belum diketahui dengan jelas. Penelitian ini menginvestigasi pengaruh MCP pada sel makrofag RAW264.7 dimana pemeriksaan dilakukan melalui pengukuran produksi sitokin dan ekspresi molekul kostimulator pada permukaan sel makrofag. Beberapa teknik pengukuran telah digunakan untuk menggambarkan mekanisme aksi pada sel makrofag seperti flowsitometri untuk menganalisa kostimulator CD80, CD86 dan MHC-II, pengukuran viabilitas sel, nitric oxide (NO) dan TNF-α menggunakan spektrofotometer, ekspresi protein ERK ½, IκB-α, iNOS menggunakan teknik western blot dan analisa aktivitas protein NF-κB menggunakan mikroskop konfokal. Pengukuran aktivitas NF-κB, ekspresi CD80, CD86, MHC-II, produksi NO dan TNF-α dilakukan setelah diberikan MCP pada sel makrofag RAW264.7 dengan konsentrasi 10, 50, 100 dan 200μg/ml. Analisa statistik dengan ANOVA menunjukkan bahwa terdapat perbedaan ekspresi NO, TNF-α, CD80, CD86 dan MHC-II pada semua kelompok perlakuan, selanjutnya uji perbandingan multipel Duncan's menyatakan bahwa terdapat peningkatan ekspresi NO, TNF-α, CD80, CD86 dan MHC-II secara signifikan (p=0,001) bila dibandingkan dengan kelompok lain. Hasil diatas menunjukkan bahwa MCP mampu menginduksi produksi NO, TNF-α, ekspresi kostimulator (CD80, CD86, MHC-II), dan sebagai stimulator terhadap protein ERK ½, IκB-α, dan NF-κB. Hal ini memberikan penjelasan terhadap mekanisme aktivitas seluler yakni melibatkan jalur MAPKs dan NF-κB yang penting dalam menginduksi produksi sitokin dan ekspresi molekul kostimulator. Dengan demikian, MCP telah menunjukkan suatu potensi sebagai agen imunostimulator dan diduga dapat menjadi suatu terapi penting pada penyakit-penyakit infeksi.

 


Keywords


makrofag; NF-κB; polisakarida Mesona chinensis

Full Text:

PDF

References


Schepetkin IA and Quinn MT. Botanical Polysaccharide: Macrophage Immunomodulation and Therapeutic Potential. International Immunopharmacology. 2006; 6(3): 317-333.

Popa V. Polysaccharide in Medicinal and Pharmaceutical Applications. 1st edition. Shawburry, United Kingdom; iSmithers; 2011.

Chen GT, Ma XM, Liu ST, Liao YL, and Zhao GQ. Isolation, Purification and Antioxidant Activities of Polysaccharides from Grifola Frondosa. Carbohydrate Polymers. 2012: 89(1): 61-66.

Benzie IFF and Galor SW. Herbal Medicine; Biomolecular and Clinical Aspects. 2th edition. New York: CRC Press; 2011; p. 453-461.

Pan H, Han Y, Huang J, et al. Purification and Identification of a Polysaccharide from Medicinal Mushroom Amauroderma Rude with Immunomodulatory Activity and Inhibitory Effect on Tumor Growth. Oncotarget. 2015; 6(19): 17777-17791.

Lv Y, Shan X, Zhao X, et al. Extraction, Isolation, Structural Characterization and Anti-Tumor Properties of an Apigalacturonan-Rich Polysaccharide from the Sea Grass Zostera Caespitosa Miki. Marine Drugs. 2015: 13(6): 3710-3731.

Wang CL, Lu CY, Pi CC, et al. Extracellular Polysaccharide Produced by Ganoderma Formosanum Stimulate Macrophage Activation Via Multiple Pattern-Recognition Receptors. BMC Complementary and Alternative Medicine. 2012: 12: 119-128.

Zhang W, Jin W, Sun D, et al. Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella Crsaaifolia. Marine Drugs. 2015: 13(3): 1360-1374.

Kang SM, Kim KN, Lee SH, et al. Anti-Inflammatory Activity of Polysaccharide Purified from AMG-Assistant Extract of Ecklonia Cava in LPS-Stimulated RAW 264.7 Macrophages. Carbohydrate Polymers. 2011: 85(1): 80-85.

O'Dea E and Hoffmann A. NF-κB signaling. Wiley Interdisciplinary Reviews: Systems Biology and Medicine. 2009; 1(1): 107-115.

Abbas AK, Litchman AH and Pillai S. Cellular and Molecular Immunology. 7th edition. Philadelphia: Elsevier Saunders. 2012; p. 55-87.

Arvaniti E, Ntoufa S, Papakonstantinou N, et al. Toll-Like Receptor Signaling Pathway In Chronic Lymphocytic Leukemia: Distinct Gene Expression Profiles Of Potential Pathogenic Significance In Specific Subsets Of Patients. Haematologica. 2011: 96(11); 1644-1652.

Shaoqin L and Sumin Z. Study on Mesona Chinensis Polysaccharide. Isolation, Purification and Identification. Natural Product Research and Development. 1992; 3.

Luo A and Fan Y. Immune Stimulating Activity of Water-Soluble Polysaccharide Fractions from Dendrobium nobile Lindl. African Journal of Pharmacy and Pharmacology. 2011; 5(5): 625-631.

Xu X, Yasuda M, Nakamura-Tsuruta S, Mizuno M, and Ashida H. Beta-Glucan from Lentinus Edodes Inhibits NO and TNF-a Production and Phosphorylation of Mitogen-Activated Protein Kinases in LPS-Stimulated Murine RAW 264.7 Macrophages. The Journal of Biological Chemistry. 2012; 287(2): 871-878.

Park SY and Kim YH. Surfactin Inhibits Immunostimulatory Function of Macrophages through Blocking NK-κB, MAPK, and Akt Pathway. International Immunopharmacology. 2009; 9(7-8): 886–893.

Buhler L, Alwayn IP, Basker M, et al. CD40-CD154 Pathway Blockade Requires Host Macrophages To Induce Humoral Unresponsiveness To Pig Hematopoietic Cells In Baboons. Transplantation. 2001; 72(11): 1759-1768.

Neumann M and Naumann M. Beyond Iκbs: Alternative Regulation 9f NF-Κb Activity. The Journal of Federation of American Societies for Experimental Biology. 2002; 21(11): 2642-2654.

Smale ST. Selective Transcription in Response to an Inflammatory Stimulus. Cell. 2010; 140(6): 833-844.

Chen L and Flies DB. Molecular Mechanism of T Cell Co-Stimulation and Co-Inhibition. Nature Reviews Immunology. 2013: 13(4): 227-242.

Yamano T, Steinert M, and Klein L. Thymic B Cells and Central T Cell Tolerance. Frontier in Immunology. 2015: 6: 5.

Ley K. The Second Touch Hypothesis: T Cell Activation, Homing and Polarization. F1000Research. 2014: 3: 37.

Chen S, Ding R, Zhou Y, Zhang X, Zhu R, and Gao XD. Immunomodulatory Effect of Polysaccharide from Marine Fungus Phoma Herbarum YS4108 on T Cell And Dendritic Cells. Hindawi Publishing Corporation. 2014; 2014: 13.

Serhan CN, Ward PA and Gilroy DW. Fundamentals of Inflammation. New York: Cambride University Press. 2010; p. 98-104.

Matsuoka T, Shamji MH, and Durham SR. Allergen Immunotherapy and Tolerance. Allergology International. 2013: 62(4): 403-413.

Cruse JM and Lewis RE. Atlas of Immunology. 3rd edition. Boca Raton, Florida: CRC Press; 2010; p. 370-372.

Hume DA. The Many Alternative Faces of Macrophage Activation. Frontiers in Immunology. 2015: 6: 370.

Yamauchi S, Ito H, and Miyajima A. Ikbη, a Nuclear Ikb Protein, Positively Regulates the NF-Kb-Mediated Expression of Proinflammatory Cytokines. Proceeding of the National Academy of Sciences of the United States of America. 2010: 107(26): 11924-11929.

Kim EK and Choi EJ. Pathological Roles of MAPK Signaling Pathway in Human Diseases. Biochimica et Biophysica Acta. 2010: 1802(4): 396-405.

Huo M, Cui X, Xue J, et al. Anti-Inflammatory Effects of Linalool in RAW264.7 Macrophages and Lipopolysaccharide-Induced Lung Injury Model. Journal of Surgical Research. 2013: 180(1): E47-E54.




DOI: http://dx.doi.org/10.21776/ub.jkb.2016.029.01.4

Refbacks

  • There are currently no refbacks.