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Kemampuan Whole Cell Helicobacter pylori dalam Menginduksi Degradasi Kolagen Tipe IV Melalui Peningkatan Aktivitas Makrofag

Yuly Peristiowati, Sumarno Sumarno, I Ketut Gede Muliarta, Murwani Murwani
  Jurnal Kedokteran Brawijaya, Vol 26, No 1 (2010),  pp.1-7  



Helicobacter pylori  berperan dalam infark miokard  akut dan penyakit jantung koroner.  Sitotoksin H.pylori  dapat menginduksi sitokin inflamasi IL1-β dan TNFα yang mengaktivasi enzim proteolitik MMP-9 dan menyebabkan degradasi kolagen melalui plak aterosklerosis. Penelitian ini dilakukan untuk membuktikan kemampuan whole cell H.pylori dalam menginduksi degadrasi kolagen tipe IV melalui peningkatan aktifitas makrofag. Aktivitas makrofag dilihat dari kadar TNFα dan IL1-β yang diukur dengan metode Elisa. Hasil uji ANOVA mengindikasikan perbedaan signifikan (p<0,001) aktifitas makrofag pada kelompok normal dan whole cell H.pylori. Produksi enzim MMP-9 dapat dideteksi melalui gelatine zymography dilanjutkan dengan western blotting pada berat molekul 93 kDa. Fragmenliasi kolagen didapatkan pada fragmen 61,2 kDa  dan 29 kDa. Dapat disimpulkan bahwa H.pylori dapat menginduksi degradasi kolagen tipe IV melalui peningkatan makrofag. Temuan ini juga mendukung patomekanisme infark miokard.Kata Kunci : Kolagen Tipe IV, makrofag, whole cell H.pylori

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Kavermann H, Burns PB, Angermuller K, et al.Identification and Characterization of Helicobacter

pylori Genes Essential for Gastric Colonization. The Journal of Experimental Medicine. 2003; 197(7): 813-822.

Mayr M, Kiechl S, Mendall MA, Willeit J, Wick G, and Xu Q. Increased Risk of Atherosclerosis Is Confine to C a g A - Po s i t i v e He l i c o b a c t e r p y l o r y S t r a i n s : Prospective Result From the Bruneck Study. Stroke. 2003; 34: 610-615. 3. Mallika V , Goswami B, and Rajappa M. Atherosclerosis Phatophysiology and the Role of Novel Risk Factor : A Clinicobiochemical Perspective. Angiology. 2007: 58; 513.

Alavarez A, Ibiza S, Hernandez C, et al. Gastrin Induces Leukocyte-endothelial Cell Interactions In Vivo and Cont r i butes to t he I nf l ammat i on Caus ed by Helicobacter pylori. The Journal of the Federation of American Societies for Experimental Biology . 2006; 20; E1742-E1752.

Danesh J and Peto R. Risk Factors for Coronary Heart Disease and Infection with Helicobacter pylori: Meta-analysis of 18 Studies. British Medical Journal. 2009; 316: 1130-1132.

Yamaoka Y , Dong HK, and Graham DY . A Mr 34,000 Proinflammatory Outer Membrane Protein (oipA) of Helicobacter pylori. Proceedings of the National Academy of Sciences of the United States of America. Stanford, April 26, 2002.

Dollery CM, Owen CA, Sukhova GK, Krettek A, Shapiro SD, and Libby P . Neitrophil Elastase in Human Atherosclerotic Plaque : Production by Macrophages. Circulation. 2003; 107: 2829-2836.8. Visse R and Nagase H. Matrix Metalloproteinases and Tissue Inhibitiors of Metalloproteinases; Structure, Function, and Biochemistry. Circulation Research. 2003; 8: 827- 839.

Ameriso SF , Fridman EA, Leiguarda RC, and Sevlever GE. Detection of Helicobacter pylori in Human Carotid Atherosclerosis Plaquaes Editorial Comment. Stroke. 2001; 32: 385-391.

Loftus IM, Naylor R, Goodal S, et al. Increased Matrix Metalloproteinase -9 Activity in Unstable Carotid Plaques: A Potential Role in Acute Plaque Disruption. Stroke. 2003; 31: 40.

Sheikhian A, Hassan ZM, Mustafaie A, Sokri F , Malekzadeh R, and Siavoshi F . Extraction of the Outer Membrane Protein of Helicobacter pylori and Evaluation of Their Presence in Stool of the Infected Individuals. Iranian Biomedical Journal. 2004; 8(2): 83-88.

Solnick JV , Hansen LM, Salama NR, Boonjakuakul JK, and Syvanen M. Modification of Helicobacter pylori Ou t e r Me mb r a n e P r o t e i n E x p r e s s o n Du r i n gExper i mental I nfect i on of Rhesus Macaques . Proceedings of the National Academy of Sciences of the United States of America. Stanford, December 22, 2003.

Ge ZM, Doig P , dan Fox JG. Characterization of Protein in the Outer Membrane Preparation of a Murine Pathogen Helicobacter bilis. Infection and Immunity. 2001: 3502-3506.

Abbas AK and Licthman AH. Cellular and Moleculer Imunology. Fifth edition. Philadelphia: The Curtis Center; 2007; p. 296-349.

Dostal DE. Matrix and Cell Adhesion Mediated Signaling. Journal Moleculer Cardiology. 2006

Weber TJ, Smallwood HS, Kathmann LE, Markillie LM, Squier TC, and Thrall BD. Functional Link between TNF Biosynthesis and CaM-dependent Activation ofInducible Nitric Oxide Synthase in RAW 264.7Macrophages. American Journal Physiology-CellPhysiology . 2006; 290: C1512-C1520.

Eskandarian R, Moosavi S, Babai M, et al. Impact of Helicobacter pylori on Prognosis of Patients with Acut Coronary Syndrome. ARY A Atherosclerosis. 2006; 1(3): 164-169.

Davi G, Neri M, Falco A, et al. Helicobacter pylori Infection Cause Persistent Platelet ctivatio In Vivo Through Enhance Lipid Peroxidation. Arteriosclerosis, Thrombosis, and Vascular Biology . 2005 ; 25: 246-251.

Andersson A, Kokkola R, Wefer J, Erlandsson-Harris H, and Harris RA. Defferential Macrophage Expression of IL-12 and IL-23 Upon Innate Immune Activation Defines Rat Autoimmune Susceptibility. Journal of Leukocyte Biology. 2004; 76: 1118-1124.

Hansson GK. Immune Mechanisms in Atherosclerosis. Arteriosclirosis, Thrombosis, and Vascular Biology . 2001; 21: 1876- 1915.

Worthly SG, Osende JI, Helft G, Badimon JJ, and Fuster V . Coronary Artery disease; Pathogenesis and Acute Coronary Syndrome. Mount Sinai Journal of Medicine. 2001.

Pasterkamp G and Daemen M. Circulating Cells : The Biofactory for Markers of Atherosclerotic Disease. European Heart Journal. 2008; 29(22): 2701-2702.

Volanen L, Raitakari OT , Vainionpa R, et al. Serum Lipid Profiles Poorly Correlate with Clamydia pneumoniae, H e l i c o b a c t e r p y l o r i , a n d C y t o me g a l o v i r u s Seropositivity in Prospectively Followed-up Healthy Children. Arteriosclirosis, Thrombosis, and Vascular Biology. 2005 ; 25: 827-832.

Amieva MR, Salama NR, T ompkins LS, and Falkov S. Hel i cobacter pyl or i Enter and Sur vi ve wi t hi n Multivesiculer Vacuoles of Ephithelial Cells. Cellular Mikrobiology. 2002; 4(10): 677.



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