User
Information for Reviewer
Index

JKB telah terindex di Crossref JKB telah terindex di Google Scholar JKB telah terindex di SINTA Ristekdikti JKB telah terindex di Portal Garuda

Notifications
Journal Content

Browse
Visitor Counter

 

DETEKSI BAKTERI Klebsiella pneumoniaePADA SPUTUM DENGAN METODE IMUNOSITOKIMIA MENGGUNAKAN ANTI OUTER MEMBRANE PROTEIN BERAT MOLEKUL 40 KDA Klebsiella pneumoniaeSEBAGAI ANTIBODI

Joko Susilo, Teguh R. Sartono, Sumarno Sumarno
  Jurnal Kedokteran Brawijaya, Vol 20, No 1 (2004),  pp.12-18  

Abstract


Klebsiella pneumoniae (K. pneumoniae) is a normal flora in human Gastro Intestinal Tract. This strain of K.pneumoniae are implicated the gram negative bacteria that can cause pneumoniabeside urinary tract infection and nosocomial infection. Until now, to identify this bacteria there are seveval methods had been used included staining and culture, Elisa, histologic and serologic that need a long time. The goal of this research is to detect K. pneumoniae with immunocytochemistry methode using antibody of K.pneumoniae and OMP K.pneumoniae as spesific antigen. The design of this reseach was explorative descriptive. The method content of K.pneumoniae isolation, specific antigen of OMP K.pneumoniae isolation, producing antibody anti OMP K.pneumoniae, and detection this bacteria on sputum with immunocytochemistry methode respectivelly. For diagnostic test sensitivity and specificity. Analysis has been used. The result showed that molecular weight of OMP K.pneumoniae was 40 kDa, antibody of OMP K.pneumoniae couldidentify the OMP K.pneumoniae as antigen and could detect K.pneumoniae on sputum by using Western Blotting and immunocytochemistry. Sensitivity and specificity of this diagnostic testwas 90 %, 95 % consequently. Key words: Klebsiella pneumoniae, Outer Membrane Protein, Immunocytochemistry

Full Text:

PDF

References


DAFTAR KEPUSTAKAAN

Dzen SM. Dasar-dasar Mikrobiologi. Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Brawijaya Malang. 1994.

Priyanti ZS. Penatalaksanaan Mutakhir Pneumonia Komuniti. Bagian Pulmonologi FKUI / RSUP Persahabatan. Jakarta; 2002.

Sartono dan Sumarno. Gambaran pola kuman pada pemeriksaan sputum di Rumah Sakit Umum Dr. Saiful Anwar Malang. 2002(Unpublish).

Sudigdo S. Dasar-dasar Metode Penelitian Klinis. Bagian Ilmu Kesehatan Anak FKUI. Jakarta; 1995.

Sumarno. Karakterisasi Molekul Protein Adesi Vibrio Cholera 01 M094 V dan Protein Reseptornya pada Sel Epitel Usus Halus Tikus Putih (Wistar). Studi Patogenesis Vibrio Cholera 01 M094V. Program Pasca Sarjana Universitas Airlangga. Surabaya. 2000.

Kisra K, et.al. Relationships among Capsular Structure, Phagocytosis, and Mouse Virulence in Klebsiella pneumoniae.Infection and Immunity; Mar. 1995; 847-852.

Keith, Miller. Immunocytochemical Techniques. Theory and Practice of Histological Techniques. 5th edition. Toronto; 2002;421-458.

Dzen SM. Enterobacteriaceae & Kuman Batang Gram Negatif yanglain. Laboratorium Mikrobiologi Fakultas Kedokteran

Universitas Brawijaya Malang. 1993.

Sydney M, Finegold MD. Enterobacteriaceae in Diagnostic Microbiology. 7th edition. Toronto; 1986; 397-421.

Freeman BA. Textbook of Microbiology 22th edition. Japan; 1985; 34-35.

P Ustian T. The Gram Negative Cell Wall. University of Wisconsin Madison199: 1-3.

Brooks GF, Botel JS, Ornston L. Medical Microbiology. Conectitut. USA. 19th edition; 1989; 15-16, 19-20.




DOI: http://dx.doi.org/10.21776/ub.jkb.2004.020.01.3

Refbacks

  • There are currently no refbacks.