IDENTIIFKASI PROTEIN IMUNOGENIK CHLAMYDIA PNEUMONIAE TERHADAP SERUM PENDERITA INFARK MIOARD AKUT

Authors

  • Sri Murwani Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Brawijaya Malang
  • Dwi Yuni Nur Hidayati Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Brawijaya Malang

DOI:

https://doi.org/10.21776/ub.jkb.2007.023.02.6

Abstract

Chlamydia pneumoniae is human respiratory tract pathogen and recently investigated as pathogen causingatherosclerosis and acute myocardial infarction (AMI). This research was carrier out to detect proteinpattern of C. pneumoniae, and to study it relation to AMI throughdetection of immunogenic protein. Design research  was laboratory observational and analyzed descriptively. The subject was C. pneumoniae. Protein pattern the bacteria was detected by electrophoresis method, and to detect the immunogenic protein was done immunoblotting. Serum was obtained from AMI patients in Saiful Anwar and Lavallette hospitals.  The result showed, protein pattern C. pneumoniae wasprotein with molecular weight 117, 107, 97, 91, 86, 61, 58, 52, 46, 44, 34, 23, 19, 9, 5, 4 kDa. Immunogenic proteins vary between AMI patients was 117, 107, 86, 61, 58, 52, 46, 44,  34 kDa. Non immunogenic proteins were 97, 91, 23, 19, 9, 5 and 4 kDa. Protein 61 kDa react to all of patient’s serum. It was concluded, C. pneumoniae have protein fractions 117, 107, 97, 91, 86, 61, 58, 52, 46, 44, 34, 23, 19, 9, 5, and 4kDa. Immunogenic proteins vary between AMI patients was 117, 107, 86, 61, 58, 52, 46, 44, 34 kDa, and 61 kDa was the  immunodominant protein. The result proved C. pneumoniae as causative agent of atherosclerosis and acute myocardial infarction, in Indonesia particularly. Key words:C. pneumoniae, immunogenic, AMI

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References

DAFTAR KEPUSTAKAAN

Campbell LA, Kuo C-C, Grayston JT. Chlamydia pneumoniae and Cardiovascular Disease. Emerging Infectious Disease, October-December 1998; 18(4).

Kaukoranta-Tolvanen SS, Ronni T, Leinonen M, Saikku P, Laitinen K. Expression of Adhesion Molecules on Endothelial Cells Stimulated by Chlamydia pneumoniae.Microb Pathog 1999;21(5):407-411

Rodel J, Woytas M, Groh A, Schmidt K-H, Hartmann M,Lehmannm, Straube E. Production of Basic Fibroblast Growth Factor and Interleukin 6 by Human Smooth Muscle Cells following Infection with Chlamydia pneumoniae. Infection and Immunity, 2000; 3635-3641

Sherer Y and Shoenfeld Y. Archieved Reports. Report on the Europen Atherosclerosis Society Workshop on Immune System in Atherosclerosis. 2001.http://www.rheuma21st.com/archives/shoenfeld_immune_sys_atheroscleosis%20.html

Christiansen G, Boesen T, Hjerno K, Daugaard L, Mygind P, Madsen AS, Knudson K, Falk E, Birkelund S. Molecular biology of Chlamydia pneumoniae surface protein andtheir role in immunopathogenicity. Denmark: Department of Medical Microbiology and Immunology and Department of Molecular and Structural Biology, University of Aarbus, Lake Diagnostic, University Hospital Aarbus. 1999.

Yamaguchi H, Haranaga S, Widen R, Friedman H, Yamamoto Y. Chlamydia pneumoniae Infection Induce Differentiation of Monocytes into Macrophages. Infection and Immunity, 2002;70(5):2392-2398

Ngeh J and Gupta S. Chlamydia pneumoniae and Atherosclerosis: Causal or Coincidental Link?,ASM News,2000; 66 (12)

Byrne GI and Kalayoglu MV. Chlamydia pneumoniae and atherosclerosis: Link to diseases process.Madison:Department of Medical Microbiology & Immunology, University of Wisconsin. 1999.

Mayr M, Metzler B, Kiechl S, Willeit J, Schett G, Xu Q, Wick G. Endothelial Cytotoxicity Mediated by Serum Antibodies to Heat Shock Proteins of Escherichia coli and Chlamydia pneumonia.Institute for Biomedical Aging Research, Austrian Academy Sciences Innsbruck; Department of Neurology, University Cinic, Innsbruck; Institute for General and Experimental Pathology, University of Innsbruck, Medical School, Innsbruck, Austria. E-mail IBA@oaeaw.ac.at

Kol A, Sukhova G.K, Lichtman, AH, Libby P. Chlamydial Heat Shock Protein 60 Localizes in HumanAtheroma and Regulates Macrophage Tumor Necrosis Factor-αand Matrix Metalloproteinase Expressin. Circulation, 1998; 300-307.

Schoenbeck U, Mach F, Sukhova GK, Murphy C, BonnefoyJ-Y, Fabunmi RP, Libby P. Regulation of Matrix Metalloproteinase Expression in Human Vascular Smooth Muscle Cells by T Lymphocytes, A Role for CD40 Signaling in Plaque Rupture?Circulation Research, 1997; 81: 448-454.

Huang Y, Mironova M, Lopes-Virella MF. Oxidation LDL Stimulates Matrix Metalloproteinse-1 Expresion in Human Vascular Endothelial Cells.Atherosclerosis, Thrombosis, Vascular Biology, 1999;19:2640

Vehmaan-Kreula P, Puolakkainen M, Sarvas M, Welgus HG, Kovanen PT. Chlamydia pneumoniae Protein Induce Secretion of the 92-kDa Gelatinase by Human Monocyte-Derived Macrophages. Atheriosclerosis, Thrombosis, and Vascular Biology, 2001;21:ie

Murwani S, Muliartha K, Ali M, Purwanto, SusilowatiIDA, Yuniarti, Nur’aini DD. Studi seroepidemiologi Infark Miokardial Akut (IMA) yang terkait dengan infeksi Chlamydia pneumoniae dan adanya infeksi multiple dengan mikroorganisme lain yang diduga dapat menyebabkan IMA.Malang: Pasca Sarjana Universitas Brawijaya. 2001.

Ciervo A, Visca P, Petrucca A, Biasucci LM, Maseri A, Cassone A. Antibodies to 60-Kilodalton Heat Sock Protein and Outer Membrane Protein-2 of Chlamydia pneumoniae inPatients with Coronary Heart Disease. Clinical and Diagnosis Laboratory Immunology, 2002; 9(1): 66-74

Kuo A, Sukhova GK, Lichtman AH, Libby P. Chlamydial HeatShock Protein 60 Localize in Human Atheroma and Regulates Macrophage Tumor Necrosis Factor-αand Matrix Metalloproteinase Expression. Circulation; 1998;98:300-307.

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Published

2013-04-17

Issue

Vol. 23 No. 2 (2007)

Section

Research Article

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