HUBUNGAN ANTARA KADAR ENZIM GLUTATION REDUKTASE DENGAN DERAJAT KEKERUHAN INTI LENSA

Authors

  • T Budi Sulistya Laboratorium Ilmu Penyakit Mata Fakultas Kedokteran Unibraw / RSU dr. Saiful Anwar Malang
  • Debby Shintiya Dewi Laboratorium Ilmu Penyakit Mata Fakultas Kedokteran Unibraw / RSU dr. Saiful Anwar Malang
  • Hidayat Suyuti Laboratorium Biokimia Fakultas Kedokteran Unibraw Malang
  • Sumarno Sumarno Laboratorium Mikrobiologi Fakultas Kedokteran Unibraw Malang

DOI:

https://doi.org/10.21776/ub.jkb.2006.022.01.7

Abstract

The aim of this study was to determine the correlation between the level of glutathione reductase [GR] in relation with the severity of nuclear cataract and the increase of age. An analytic cross sectional observasional study  of nuclear cataract patient seen at Saiful Anwar Hospital and Mass Cataract Surgery performed by Perdami Malang was carried out. Patients were classified into 3 groups based on ageand 4 groups based on severity of nuclear cataract. The samples were taken by consecutive sampling and were operated on visual indication. The extracted lens were examined for [GR]. The laboratory findings of [GR] were compared with the severity of senile cataract. The data were analyzed with one way ANOVA. Confidence limit was set at 95%. A total of  55 patients were included with the sample size in 40-50 age group less than estimation. The [GR] were found to be significanfly lower in the older age (p=0,01) and the more severe cataract groups (p=0,00). The [GR] were also found to be significantly decreased with the increase of cataract severity in 40-50 age group (p=0,00), 50-60 age group (p=0,01) and >60 age group (p=0,00). In the grade 2 cataract, there were no significant difference of [GR] between age group (p=0.22). This also occured in grade 3 (p=0.23) while  in the grade 4, there were significant difference between age group (p=0.01). This also occured in grade 5 (p=0.06).

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References

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Leske M C, Chylack LT, He Q, et al. Antioxidant Vitamins and Nuclear Opacities- The Longitudinal Study of Cataract. Ophthalmology 1998; 105: 831-836.

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Forrester J V. Aging and Vision. Br J Ophthalmol 1997;81:809-10.

Refa Safaruddin. Pelayanan Katarak Terpadu. Bagian Ilmu Penyakit Mata FK Unibraw/ RSSA Malang,2001: 1-2.

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Hood BD, Garner B, Truscott RJW. Human Lens Coloration and Aging: Evidence for Crystallin Modification by The Major Ultraviolet Filter, 3-Hydroxy-Kynurenine O-D-Glucoside. J Biol Chem. 1999. 274(46): 32547-50.

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Kidd PM. Glutathione: Systemic Protectant Against Oxidative and Free Radical Damage. Alt Med Rev.1997;2(3):155-76.

Mayes PA. Metabolisme Karbohidrat. In: David W Martin, Jr. Alih Bahasa: Iyan Darmawan. Biokimia. Ed 20, Jakarta:EGC,1987;15:202-4.

Fred MW. Practical Ophthalmology: Amanual for Beginning Residents. San Francisco: American Academy of Ophthalmology,1996;10:220-1.

Kuzma JW, Bohnenblust. Basic Statistics for the Health Sciences. 5th Ed. Boston: Mc Graw- Hill, 2005; 7: 106-122.

American Academy of Ophthalmology staff. Lens and Cataract. In: Basic and Clinical Science Course. Section 11. San Francisco: The Foundation of the American Academy of Ophthalmology, 2003: 5-49.

Buratto L. Cataract Surgery Development and techniques. In: Lucio Buratto. Phacoemulsification: Principles and techniques. Thorofore: SLACK, Inc,1998; 1:5-7.

Budiman. Transisi dari Ekstraksi Katarak Ekstrakapsular ke Fakoemulsifikasi. In: Kumpulan naskah SeminarBedah Katarak Incisi kecil dan Fakoemulsifikasi. Semarang:Perdami cabang Jawa Tengah., 2001.

Datiles MB, Kinoshita JH. Pathogenesis of Cataracts. In: William Tasman, Edward A. Jaeger. Duane’s Clinical Ophthalmology. Volume 1. Philadelphia: J.B. Lippincott Company,1994;72B:1-14.

Fu S, Dean R, Southan M, Truscott R. The Hydroxyl Radical in Lens Nuclear Cataractogenesis. The Journal of Biological Chemistry, 1998,.273, 44, 28603-9.

Leske M C, Chylack LT, He Q, et al. Antioxidant Vitamins and Nuclear Opacities- The Longitudinal Study of Cataract. Ophthalmology 1998; 105: 831-836.

Sperduto. Epidemiologic Aspects of Age-Related Cataract. In: William Tasman, Edward A. Jaeger. Duane’s Clinical Ophthalmology. Volume 1. Philadelphia: J.B. Lippincott Company, 1994; 73 A: 1-2.

Gondhowiardjo TD. Aktifitas Enzim Aldehid Dehidrogenase pada Lensa Katarak Diabetes dan Non-Diabetes. Ophthalmologica Indonesiana 1996; 16(2): 118-24.

Apple et al. Survey of Ophthalmology.45(1)2000. S13-S21.

Forrester J V. Aging and Vision. Br J Ophthalmol 1997;81:809-10.

Refa Safaruddin. Pelayanan Katarak Terpadu. Bagian Ilmu Penyakit Mata FK Unibraw/ RSSA Malang,2001: 1-2.

Suhendro G. Pembentukan Sikatrik Retina Perifer Pada Nonproliferatif Retinopati Diabetik Untuk Mempertahankan Sistem Autoregulasi Retina Sentral (dissertation).Surabaya; Universitas Airlangga; 1999.

Hood BD, Garner B, Truscott RJW. Human Lens Coloration and Aging: Evidence for Crystallin Modification by The Major Ultraviolet Filter, 3-Hydroxy-Kynurenine O-D-Glucoside. J Biol Chem. 1999. 274(46): 32547-50.

American Academy of Ophthalmology staff. International Ophthalmology. In: Basic and Clinical Science Course. Section 13. San Francisco: The Foundation of the American Academy of Ophthalmology, 2003: 157-66.

Delamere NA, Paterson CA. The Crystalline Lens. In: William Tasman, Edward A. Jaeger.Duane’s Clinical Ophthalmology 2. Philadelphia: J.B. Lippincott Company,1994;10:1-19.

Linetsky M, Chemoganskiy V G, Hu Fang, Ortmerth BJ. Effect of UV A Light on the Activity of Several Aged Human Lens Enzymes. Invest. Ophthalm. And Vis Scie. 2003; 44: 264-74.

Bova LM, Sweeney MHJ, Jamie JF, Truscott RJW. Major Changes in Human Ocular UV Protection with Age.Invest. Ophthalm. And Vis Scie. 2001;42:200-5.

Vasquez S, Nicole R P, Sheil M, Truscott JW. Protein Bound Kynurenine Decreases with the Progression of Age-Related Nuclear Cataract. Invest Ophthalmol Vis Sci. 2004;45:879-83.

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Published

2013-04-18

Issue

Vol. 22 No. 1 (2006)

Section

Research Article

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