Outer Membrane Protein 49,4 Kda dari Porphyromonas gingivalis Merupakan Protein Hemaglutinin dan Adhesin Terhadap Netrofil

Authors

  • Siti Nurul Mubarokah Program Pascasarjana, Fakultas Kedokteran Universitas Brawijaya rogram Studi Biomedik
  • Sumarno Sumarno Laboratorium Mikrobiologi, Fakultas Kedokteran Universitas Brawijaya
  • I Ketut Gede Muliartha Laboratorium Biomedik, Fakultas Kedokteran Universitas Brawijaya

DOI:

https://doi.org/10.21776/ub.jkb.2009.025.02.6

Abstract

ABSTRACTPeriodontitis is caused mostly by P. gingivalis and it is related to acute coronary syndrome. P. gingivalis readilyinvades into blood circulation and potentially induces. Collagenolytic activity of neutrophil which result incollagen vascular degradation lead to atherosclerotic plague rupture (APR). APR is responsible for occurringfatal cardiovascular events such as acute myocardial infarction (AMI). These information brought out notionconcerning the adhesion interaction of P. gingivalis with neutrophil. The aim of this study was to assessadhesion molecule of P. gingivalis outer membrane protein (OMP) by partial characterization took inhemagglutination assay using mice erythrocytes, adhesion inhibition assay by gradual concentration ofadhesion blocked in neutrophil, immunologic assay using Western-blotting and immunocytochemistry. Theresults showed that 49,4 kDa P.gingivalis OMP can agglutinate mice erythrocytes and adherence to netrophilIncrease concentration of OMP P. gingivalis 49,4 kDa reduce adhesion process to netrophil This protein wasrecognized by the polyclonal antibody of 49,4 kDa adhesion molecule. It can be concluded that P gingivalisouter membrane protein is a hemagglutinin and an adhesion molecule to netrophilKeywords : 49,4 kDa P. gingivalis OMP, neutrophil, hemagglutination, adhesin

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References

Joesoef, H. Andang, Setianti, Budhi. Hipertensi sekunder. Buku Ajar Kardiologi. Jakarta; FK UI. 2003.

Constantinides P. General pathobiology. Appleton & Lange Norwalk. Connecticut; US. 1994

Sumarno, Noorhamdani AS, Samsul Islam, Sjoekoer M. Dzen, Ehara M, dan Ichinose Y. Purifikasi protein hambatan aglutinasi Vibrio cholera El Tor. Majalah Kedokteran Universitas Brawijaya. 1991;10(5): 179-186.

Susilawati IDA. Induksi Porphyromonas gingivalis terhadap aktivitas kolagenolisis netrofil pada kolagen tipe IV (studi in vitro mekanisme kolagenolisis plak aterosklerotik) . [Disertasi]. Malang: Universitas Brawijaya. 2008

Lamont RJ, Jenkinson HF. Life below the gum kolom: patogenic mechanism of porphyromonas gingivalis.. Microbiology ang Molecular Biology Review.1998; 62 (4):1244-1263.

Deshpande RG, Khan MB, Genco CA. 1999. Invasion of aortic ang heart endothelial cells by Porphyromonas gingivalis. Infection and Immunity. 1999; 66(11): 5337-5343.

Dorn BR, Dunn WA, Fox AP. Invasion of human coronary artery cells by periodontal patogens Infection and Immunity. 1999; 7(11): 5792-5708.

Chiu B. Multiple infection in carotid atherosclerotic plaques. Am Heart J. 2001;138(5 Pt 2): 534-6.

Haraszthy VI, Zambon JJ, Trevisan M, Zeid M Genco RJ. Identification of periodontal patogens in atheromathosus plaques . . J Periodontol2000; 71 : 1554-60.

Stelzel M , Conrads G , Pankuweit S , Maisch B , Vogt S, Moosdorf R, Flores-de-Jacoby L. 2002. Detection of Porphyromonas gingivalis DNA in aortic tissue by PCR. . J Periodontol. 2002 Aug; 73(8): 868-70.

Todar K. The Mechanisms of bacterial patogenicity. Departement of Bacteriology. University of Wisconsin; 2002

Sumarno. Karakterisasi molekuler protein adhesi Vibrio cholera V.cholerae01 MO94 dan protein reseptornya pada sel epitel usus halus tikus putih (Wistar) Studi Patogenesis 01 MO94. [Disertasi]. Surabaya: Program Pascasarjana Universitas Airlangga. 2000.

Ekpo, Pattama., Utane, . et al Expression and purification of 30 Kilodalton protein antigen of ara-Burkholderia pseudomallei. Southeast Asian J Trop Med Public Health. 2009; 40(2): 295-301.

Winarsih S, Sumarno, Roekistiningsih. Kajian fungsi dan sifat imunogenisitas protein hemaglutinin 32 kDa dan 20 kDa pada Helicobacter pylori. Majalah Kedokteran Universitas Brawijaya 1998.

Finkelstein RA and Henne LF. 1982. Characterization and distribution of the hemaglutinins produced by Vibrio cholera. Infect Immun. 1982; 36: 209-269.

Romanelli R, Mancini S, Laschinger C, Overall CM, Sodek J, McCulloch CAG. Activation of neutrophil collagenase in perodontitis. Infection and Immunity. 1999; 69(5): 2319-2326.

Towbin H, Stahelin T, and Gordon J. Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets. Proc. Nat. Acad. Sci. USA. 1979; 76: 4350-4354.

Romanelli R, Mancini S, Laschinger C, Overall CM, Sodek J, McCulloch CAG. Activation of neutrophil collagenase in perodontitis. Infection and Immunity. 1999; 69(5): 2319-2326.

Condorelli F, Scalis G, Cali G, Rosseti B, Nicoletti a n d B l u e A M L . 1 9 9 8 . I s o l a t i o n o f Porphyromonas gingivalis and detection of immunoglobulin a specific to fimbrial antigen in gingival crevicular fluid. JCM. 1998; 36(8): 2322-2325.

Nagayama K, Oguchi T, Arita M, Honda T. Purification and characterization of a cell a s s o c i a t e d h e m a g g l u t i n i n o f V i b r i o parahaemolyticus. Infec. Immun. 1995; 63(5) : 1987-1992.

Nelson KE, Fleischmann RD, DeBoy RT, et al. Complete genome sequence of the patogenic bacterium Porphyromonas gingivalis strain W83 Journal of Bacteriology. 2003; 185(18): 5591-5601.

Hiratsuka K, Yoshida W, Hayakawa M, Takiguchi H, Abiko Y.' Polymerase chain reaction and an outer membrane protein gene probe for the detection of Porphyromonas gingivalis. FEMS Microbiol Lett. 1996; 138(23): 16772.

Zhang G, Neubert. Use of detergent to increase selectivity of immunoprecipitation of tyrosine phosphorilated peptides prior to identification by MALDI quadrupole-TOF MS . . Proteomics 2006; 6(2): 571-578

Mc. Garrey DJ, Alfred DR. Characterization of a cell associated hemagglutinin of Vibrio parahaemolyticus. Infec.Immun. 1994; 62(10): 4587-4593.

Mouton C, Ni ED, Deslauriers M, Lamy L. The h e m a g g l u t i n a t i n g a d h e s i n H A - A g 2 o f Bacteroides gingivalis is distinct from fimbrilin. Oral Microbiol Immunol. 1991; 6(1): 6-11

Lepine J, Messner HA. Pluripotent hemopoietic p r o g e n i t o r s ( C F U - G E M M ) i n c h r o n i c myelogenous leukemia The International Journal of Cell Cloning. 1996; 1(4): 230-239

Progulske-Fox A, S. Tumwasorn, and S. C. Holt. The expression and function of a Bacteroides gingivalis hemagglutinin gene in Escherichia coli. Oral Microbiol. Immunol. 1989; 4 121131.:

Alam M, Miyoshi SI, Tomochika KI, Shinoda S. Purification and characterization of novel hemagglutinin from Vibrio mimicus : 39-kDa m a j o r o u t e r m e m b r a n e p r o t e i n a n d lipopolycacharide. Infec.Immune. 1996; 64: 4035-4041.

Hiroaki, Tagawa, Kiyama K, Michiko, Sheng YL, Abiko Y. Inhibitation of hemagglutinating activity by monoclonal antibody againts Porphyromonas gigivalis 40-kDa outer membrane protein Hybridoma and Hybridomics. 2004; 23(3)

Abraham SM, Sharom N, Ofek L. Adhesion of bacterial to mucosal surfaces. Di dalam Sanarto, S. Laporan Penelitian Disertasi. Pascasarjana Universitas Airlangga. Surabaya; 1999

Wu, J Diep, TS Ho VA. Quantification of bacteria in blood of typhoid fever patients and relationship between countd and clinical features, transmissibility and antibiotic resistance. J. Clim Microbiol. 1999; 70: 551-557.

Goldhar, J. Bacterial lectinlike adhesions : determination and specificity in bacterial pathogenesis. methods in enzymology Academic Press. San Diego. 1994; 236: 211-231.

Maeba Satomi, Shigeo Otake, Jun Namikoshi, . et al. 2005. Transcutaneous immunization with a 4 0 - k D a o u t e r m e m b r a n e p r o t e i n o f Porphyromonas gingivalis induces specific antibodies which inhibit coaggregation by P. gingivalis. J Vaccine. 2005; 23: 2513-2521.

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Published

2013-03-15

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Research Article