Ekspresi IFN-γ dan IL-4 CD4+T Limfosit pada Tuberkulosis Kontak terhadap Antigen 38 Kda Mycobacterium tuberculosis

Authors

  • Iin Trilistiyanti Nugrahani Bagian Ilmu Kesehatan Anak, Universitas Brawijaya, RSUD dr Saiful Anwar, Malang
  • HMS Chandra Kusuma Bagian Ilmu Kesehatan Anak, Universitas Brawijaya, RSUD dr Saiful Anwar, Malang
  • Tri Yudani Mardining Raras Lab Biomedik & Biokimia FKUB
  • Maimun Zulhaidah Arthamin Lab PK FKUB/RSSA
  • Tri Wahju Astuti Lab/SMF Paru FKUB/RSSA
  • Francisca Tanoerahardjo Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan, Indonesia

DOI:

https://doi.org/10.21776/ub.jkb.2015.028.04.8

Abstract

Mengendalikan epidemi tuberkulosis pada anak adalah prioritas kesehatan global. Efikasi vaksin Bacillus of Calmette and Guerin (BCG) sangat bervariasi sehingga memerlukan pengembangan vaksin baru. Antigen rekombinan 38 Kda Mycobacterium tuberculosis sebagai kandidat vaksin harus melalui uji imunogenitas. Tujuan studi ini untuk mengidentfikasi apakah antigen  38 Kda dapat menstimuli ekspresi IFN-γ dan IL-4 limfosit TCD4+ pada kultur PBMC anak dengan kontak TB. Studi ini dilakukan pada kultur PBMC dari 8 kontak TB dan 8 anak sehat yang diinduksi oleh antigen 38 Kda (kelompok 1), PPD (kelompok 2) dan tanpa perlakuan (kelompok 3). Ekspresi IFN-γ dan IL-4 limfosit TCD4+ diukur dengan flowcytometry. Rerata kadar IFN-γ untuk kontak TB tertinggi pada kelompok 3 (p=0,76), sedangkan rerata IL-4 tertinggi pada kelompok 2(p=0,68).RerataIFN-γ untukkelompok sehattertinggi pada kelompok3(p=0,78) sedangkanrerata IL-4 tertinggi pada kelompok 2 (p=0,32). Rerata ekspresi IFN-γ dan IL-4 yang diinduksi oleh antigen 38 Kda, masing-masing lebih tinggi pada kontakTB daripada subjek sehat (p=0,62 dan 0,39). Pengaruh respon imun yang protektif ditunjukkan oleh rasio ekspresi IFN-γ dan IL-4 yang lebih dari 1, baik pada kontak TB dan sehat (1,22 dan 1,28). Tidak ada perbedaan signifikan antara perlakuan pemberian antigen38 kDa, PPD dan tanpa perlakuan. Dapat disimpulkan bahwa antigen 38 Kda dapat menstimuli  ekspresi IFN-γ dan IL-4 limfosit TCD4+pada kultur PBMC kontak TB. Kata Kunci: Antigen rekombinan 38 Kda, Mycobacterium tuberculosis, IFN-γ, IL-4, limfositTCD4+

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Author Biographies

Iin Trilistiyanti Nugrahani, Bagian Ilmu Kesehatan Anak, Universitas Brawijaya, RSUD dr Saiful Anwar, Malang

LAB/SMF IKA FKUB/RSSA

HMS Chandra Kusuma, Bagian Ilmu Kesehatan Anak, Universitas Brawijaya, RSUD dr Saiful Anwar, Malang

Bagian Ilmu Kesehatan Anak, Universitas Brawijaya, RSUD dr Saiful Anwar, Malang

Tri Yudani Mardining Raras, Lab Biomedik & Biokimia FKUB

Lab Biomedik & Biokimia FKUB

Maimun Zulhaidah Arthamin, Lab PK FKUB/RSSA

Lab PK FKUB/RSSA

Tri Wahju Astuti, Lab/SMF Paru FKUB/RSSA

Lab/SMF Paru FKUB/RSSA

Francisca Tanoerahardjo, Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan, Indonesia

Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan, Indonesia

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Published

2015-07-08

Issue

Vol. 28 No. 4 (2015)

Section

Research Article

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