Optimation of Combination of N-Hexane Solution and Ethyle Acetate on Secondary Metabolite Compounds Profile of Streptomyces hygroscopicus

Authors

  • Dio Giovanni Ariel Study Program of Pharmacy, Faculty of Medicine, Universitas Brawijaya Malang http://orcid.org/0000-0002-5015-8100
  • Sri Winarsih Pharmacy Study Program, Faculty of Medicine, Universitas Brawijaya Malang; Doctoral Program in Medical Sciences, Faculty of Medicine, Universitas Brawijaya Malang; Malaria Research Group, Faculty of Medicine, Universitas Brawijaya Malang
  • Fitria Febriliani Putri Study Program of Pharmacy, Faculty of Medicine, Universitas Brawijaya Malang http://orcid.org/0000-0002-4535-3331
  • Nabila Erina Erwan Master Program in Biomedical Science, Faculty of Medicine, Universitas Brawijaya Malang http://orcid.org/0000-0001-9028-9861
  • Ajeng Maharani Putri Master Program in Biomedical Science, Faculty of Medicine, Universitas Brawijaya Malang http://orcid.org/0000-0001-6142-0744
  • Alfian Wika Cahyono Doctoral Program in Medical Sciences, Faculty of Medicine, Universitas Brawijaya Malang http://orcid.org/0000-0002-9279-1764
  • Kana Mardhiyyah Doctoral Program in Medical Sciences, Faculty of Medicine, Universitas Brawijaya Malang; Department of Biochemistry and Biomolecular, Faculty of Medicine, Universitas Brawijaya Malang; Malaria Research Group, Faculty of Medicine, Universitas Brawijaya Malang http://orcid.org/0000-0003-0972-2918
  • Loeki Enggar Fitri Department of Parasitology, Faculty of Medicine, Universitas Brawijaya Malang; Doctoral Program in Medical Sciences, Faculty of Medicine, Universitas Brawijaya Malang; Malaria Research Group, Faculty of Medicine, Universitas Brawijaya Malang http://orcid.org/0000-0002-4880-1048
  • Rivo Yudhinata Brian Nugraha Department of Parasitology, Faculty of Medicine, Universitas Brawijaya Malang; Malaria Research Group, Faculty of Medicine, Universitas Brawijaya Malang http://orcid.org/0000-0002-4314-6961

DOI:

https://doi.org/10.21776/ub.jkb.2021.031.03.11

Keywords:

Extraction, flash chromatography, fractionation, thin layer chromatography, Streptomyces hygroscopicus

Abstract

Streptomyces hygroscopicus (S.hygroscopicus) is a Gram-positive soil bacterium that can produce secondary metabolites from fermentation that have a therapeutic effect. The fermented S. hygrocospicus metabolites that are still in the form of crude extracts are difficult to develop as drug preparations because the active compounds are not yet known, so it will be challenging to determine the dosage of drugs that have a therapeutic effect. Therefore, it is necessary to carry out exploratory research to narrow down the secondary metabolite profile from the fermentation of S. hygroscopicus, using extraction and fractionation methods, which are then identified by Thin-Layer Chromatography (TLC) using a combination of solvents. This study used the extraction method with a separating funnel. The fractionation was carried out using the BUCHI (Sepacore®) Flash Chromatography and Reveleris® PREP Purification System column chromatography gradually using ethyl acetate and n-hexana.  47 and 60 of the fractionation results were taken as samples, that further were profiled using TLC and given the appearance of 10% KOH stains and p-Anisaldehyde - sulfuric acid, so that various classes of compounds with different Rf values were obtained, namely Monoterpenes, Triterpenes, Steroids, Saponins, Coumarin, Scopoletin, and Alkaloids.

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Published

2021-02-27

Issue

Vol. 31 No. 3 (2021)

Section

Research Article

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