Karboksimetil Kitosan Menurunkan Degranulasi Mast Cell yang Diinduksi Oleh Ovalbumin
DOI:
https://doi.org/10.21776/ub.jkb.2009.025.01.2Abstract
ABSTRACTOvalbumin is known as mast cell degranulating agent through the FCεRI aggregation. Carboxymethyl
chitosan (CMCs), a biocompatible cationic polymer was evaluated to reduces mast cell degranulation induced
by ovalbumin. In this experiment, wistar rats were d
arboxymethyl chitosan
ivided into 5 groups which consist of 1 control group and 4
allergen groups. Among the allergen groups, 3 groups were treated with carboxymethyl chitosan/CMCs
(doses : 0,25 mg, 0,50 mg and 1,00 mg) for 22 days. The result showed a significant (P<0,01) reduction in
mast cell degranulation in allergen groups. This finding indicate that CMCs possesses antiinflammation
activity mediated by reducing of mast cell degranulation.
Keywords : ,c mast cell degranulation
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DAFTAR KEPUSTAKAAN
Baratawidjaja. Immunologi dasar edisi ke tujuh Balai Penerbit Fakultas Kedokteran Universitas Indonesia; 2006
Widjajanto E. Mast cell Fakta dan potensinya dalam perspektif Laboratory Medicine. Pidato pengukuhan jabatan guru besar dalam bidang Ilmu Patologi Klinik pada Fakultas Kedokteran
Bellanti JA. . WB Saunders Immunlogy II Company: Philadelphia; 1978; 471-490.
Abraham SN. & R. Malaviya. Mast cell in infection and immunity . Infect Immun. 1997;65:3501-3508.
Krishnaswamy G, Kelley J, Johnson D, et al. The Human mast cell: Functions in physiology and disease. Bioscience. 2001;6:d1109-1127.
Kim SM, You HJ, You MK, Kim NS, Sim BS & Kim HM. Inhibitory effect of water-soluble chitosan on TNF- and IL-8 secretion from HMC-1 cells . J. Immunofpharmacology and Immunotoxicology.2004;26:401-409.
Dwiyitno, Basmal J, Mulyasari. Pengaruh Suhu Esterifikasi Terhadap Karakteristik Karboksimetil Kitosan (CMCTs) . J. Penel. Perik. Indonesia. Edisi Pascapanan. 2004;10(3):67-73.
Davies DH, Elson CM, and Hayes ER. N,O Carboxymethyl Chitosan, A New Water Soluble Chitin Derivative. In Gudmund Skjak-Braek, Anthonsen, G.T. And Sanford, P. (Eds). Chitin and Chitosan : Elsevier Applied Science; 1989;474
Muzzarelli RA Rocchetti A, Stanic RV, & Weckx M. Methods for the Determination of Degree of Acetylation of Chitin and Chitosan. In Muzzarelli, R. A. A and Peter, M. G. (eds.). Chitin Handbook. European Chitin Society. P; 1997;109-132.
Zhai M, Yoshii F & Kume. Studying on Antibacterial Starch/Chitosan Blent Film Formed Under The Action of Irradiation. J. Carbohydrate Pol; 2003; 52(3):311-317.
Nishimura K, Nishimura S, Nishi N, Saiki I, Tokura S & Azuma I . Immunological activity of chitin and derivatives. Vaccine. 1984;2:93-99.
Hsieh KY, CI Hsu, JY Lin, CC Tsai & RH Lin. Oral administration of an edible-mushrom derived protein inhibit the development of food-allergic reaction in mice. Clin Exp Allergy. 2003; 33:1595-1602.
Morokata T, J Ishikawa & T Yamada. Antigen dose the defines T helper 1 and T helper 2 responses in the lung of C57BL/6 and BALB/c mice independently of splenic responses. ImmunolLett . 2000 ;72: 119-126 .
Kubes P, Kanwar S, Niu XF & Gaboury JP. Nitric oxide synthesis inhibition induces leukocyte adhesion via superoxide and mast cell. FASEB J. 1993;7:1293-1299.
Widjajanto E. Hubungan antara mastosit dengan Hiposelularitas sumsum tulang melalui mediator leukotrien B-4 (LTB-4) dan tumor nekrosis factor- α (TNF-α), penelitian pada aspirat sumsum tulang dari penderita gangguan hematopoisis [Disertasi]. Surabaya: Program Pascasarjana Universitas Airlangga. 2001.
Gilfillan AM, & Tkaczyk CH. Integrated signaling Pathways for mast cell activation. Nat. Rev. Immunol. 2006;6:218-230
Liang X, H Wang, H Tian, H Luo & J Chang. Synthesis, structure and properties of novel quaternized carboxymethyl chitosan with drug loading capacity. Acta Physico-Chimica Sinica. 2008;24(2):223-229.
Daroz LRD, JB Lopes, LAO Dallan, SP Campanafilho, LFP Moreira & NAG Stolf. Prevention of postoperative pericardial adhesions using thermal sterile carboxymethyl chitosan; 2008
Subowo . Imunobiologi Penerbit Angkasa Bandung;1993
Maeda Y & Yoshiyuki K. Antitumor Effects of Various Low-Molecular-Weight Chitosans Are Due to Increased Natural Killer Activity of Intestinal Intraepithelial Lymphocytes in Sarcoma 180-Bearing Micem. J. Nutr. 2004;134: 945-950.
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