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Efek Waktu Paparan Genistein terhadap Pembentukan Jantung Embrio Zebrafish

Habiba Aurora, Devi Anggraeni Pramulawati, Diana Lyrawati
  Jurnal Kedokteran Brawijaya, Vol 28, No 1 (2014),  pp.22-25  

Abstract


Genistein merupakan fitoestrogen yang telah banyak diteliti manfaatnya terhadap kesehatan. Efek samping penggunaan genistein belum banyak dipelajari. Pada masa awal perkembangan paparan genistein dalam dosis tinggi dapat menyebabkan gangguan pertumbuhan dan meningkatkan angka kematian. Penelitian ini bertujuan membuktikan pengaruh perbedaan waktu paparan genistein terhadap perkembangan jantung zebrafish, melalui pengamatan pada frekuensi denyut jantung dan ketebalan pericardium dari embrio zebrafish. Embrio zebrafish dipapar dengan genistein 10 µM sejak 2, 24, dan 48 hpf (hours post fertilization). Pengamatan dilakukan pada 72 hpf pada mikroskop dengan perbesaran obyektif 40x, pengukuran ketebalan perikardium dilakukan menggunakan mikrometer pada bagian lateral embrio. Hasil penelitian ini menunjukkan bahwa paparan genistein pada tahap awal perkembangan zebrafish menyebabkan penurunan frekuensi denyut jantung dan edema perikardium. Efek tersebut akan berkurang dengan penundaan awal pemberian paparan genistein.

Kata Kunci: Denyut jantung, edema perikardium, genistein, zebrafish


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References


Sassi-Messai S, Gibert Y, Bernard L, et al. The Phytoestrogen Genistein Affects Zebrafish Development through Two Different Pathways. PLOS ONE. 2009; 4(3): e4935.

Cornwell T, Cohick W, and Raskin I. Dietary Phytoestrogens and Health. Phytochemistry. 2004; 65(8): 995-1016.

Wanibuchi H, Kang JS, and Salim EI. Toxicity vs. Beneficial Effects of Phytoestrogens. Pure and Applied Chemistry. 2003; 75(11-12): 2047-2053.

Polkowski K and Mazurek AP. Biological Properties of Genistein. A Review of In Vitro and In Vivo Data. Acta Poloniae Pharmaceutica. 2000; 57(2): 135-155.

Chakraborty C, Hsu CH, Wen ZH, Lin CS, and Agoramoorthy G. Zebrafish: A Complete Animal Model for In Vivo Drug Discovery and Develoment. Current Drug Metabolism. 2009; 10(2): 116-124.

Denvir MA, Tucker CS, and MulliasJJ. Systolic and Diastolic Ventricular Function in Zebrafish Embrios Influence of Norepinephrine, MS-222 and Temperature. Biomed Central. 2008; 8: 21.

Dixon RA and Ferreira D. Genistein. Phytochemistry. 2002; 60(3): 205–211.

Kim DJ, Seok SH, Baek MW, et al. Developmental Toxicity and Brain Aromatase Induction by High Genistein Concentrations in Zebrafish Embryos. Toxicology Mechanisms and Methods. 2009; 19(3): 251-256.

Westerfield M. The Zebrafish Book. A Guide for the Laboratory Use of Zebrafish (Danio Rerio). 4th edition. Eugene: University of Oregon Press; 2000.

Kishida M, Tchoudakova A, Miller DS, and Callard G. Analysis of the Goldfish Carassius Auratus Aromatase P450arom Gene Promoters by Green Fluorescent Protein GFP Expression in Living Zebrafish Danio Rerio Embryos. Bulletin of the Mount Desert Island Biological Laboratory. 1999; 38: 37-38.

Antkiewicz DS, Burns CG, Corney SA, Peterson RE, and Herdeman W. Heart Malformation is an Early Response to TCDD in Embryonic Zebrafish. Toxicological Sciences. 2005; 84(2): 368-377.

Hoage T, Ding Y, and Xu X. Quantifying Cardiac Functions in Embryonic and Adult Zebrafish. Methods in Molecular Biology. 2012; 843: 11–20.

Kimmel CB, Ballard WW, Kimmel SR, Ullmann B, and Schilling TF. Stages of Embryonic Development of Zebrafish. Developmetal Dynamics. 1995; 203(3): 253-310.

Babiker FA, De Windt LJ, van Eickels M, Grohe C, Meyer R, and Doevendans PA. Estrogenic Hormone Action in the Heart: Regulatory Network and Function. Cardiovascular Research. 2002; 53(3): 709–719.




DOI: http://dx.doi.org/10.21776/ub.jkb.2014.028.01.5

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