PENGHAMBATAN AKTIFASI NFÎº ÎºÎº ÎºB OLEH CAPE (CAFFEIC ACID PHENETHYL ESTER), KOMPONEN AKTIF MADU LEBAH (HONEYBEE HIVES),PADA HUVECâ€™S (HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS) YANG DIPAPAR LDL TEROKSIDASI
AbstractThe high prevalence of cardiovascular morbidity and Â mortality caused by complication of atherosclerosisneeds anÂ optimal effort to prevent the atherosclerosis progression and complication. Atherogenesis is a chronicinflammatoryÂ process which could be prevented via the inhibitionof NFÎºB, a known key transcription factor involved in inflammatoryÂ process. An active component of honeybee hives, CAPE (Caffeic Acid Phenethyl Ester), is believed to inhibitÂ inflammatory process via the inhibition of NFÎºB activation. However, it remains uncertain whetherCAPE inhibits NFÎºBÂ activation in endothelial cells. This study therefore was aimed to examine the molecular mechanism of CAPE mediatedÂ NFÎºB inactivation in OxLDL-treated HUVECâ€™s. Immunohistochemistry using p50 antibody was applied to detecttheÂ migration of NFÎºB (p50-p65 complex) from inactive form in cytoplasmto active form in nucleus.
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