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Pengaruh Ekstrak Propolis terhadap Apoptosis melalui Ekspresi Brain-Derived Neurotrophic Factor (BDNF) pada Sel Otak Tikus Model Cedera Otak Traumatik

Fathia Annis Pramesti, Hari Purnomo, Farhad Balafif
  Jurnal Kedokteran Brawijaya, Vol 29, No. 3 (2017),  pp.209-215  

Abstract



Cedera otak merupakan penyebab utama morbiditas dan mortalitas pada semua kelompok umur. Selama cedera kepala terjadi reaksi kaskade inflamasi yang nantinya akan menyebabkan apoptosis dan nekrosis. Propolis memiliki aktivitas antiinflamasi yang kuat melalui peningkatan ekspresi Brain-Derived Neurotrophic Factor (BDNF) yang akan berikatan dengan tyrosine kinase-B (trkB) yang akan menghambat apoptosis, sehingga propolis memiliki potensi sebagai alternatif terapi dalam menurunkan apoptosis melalui peningkatan ekspresi BDNF pada cedera otak traumatik. Penelitian ini bertujuan untuk menguji pengaruh pemberian propolis dalam berbagai dosis pada ekspresi BDNF dan apoptosis di sel otak tikus Rattus norvegicus model traumatik. Sampel dibagi menjadi 5 kelompok, yaitu: kelompok model trauma dan diberi perlakuan propolis masing-masing dosis 50mg, 100mg, dan 200mg, kontrol positif dan kontrol negatif. Pada akhir penelitian, tikus dikorbankan dan dibuat preparat otak untuk menilai ekspresi BDNF dan apoptosis. Berdasarkan hasil analisa statistik, didapatkan hubungan yang signifikan antara ekspresi BDNF dan apoptosis sel otak tikus model traumatik dengan berbagai dosis propolis (p=0,001, r=0,955; p=0,000, r=0,904, korelasi Pearson). Penelitian ini membuktikan bahwa propolis berpengaruh dalam peningkatan ekspresi BDNF dan penurunan apoptosis di sel otak tikus model traumatik.
Kata Kunci: Apoptosis, BDNF, cedera otak traumatik, propolis


Keywords


Apoptosis; BDNF; cedera otak traumatik; propolis

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DOI: http://dx.doi.org/10.21776/ub.jkb.2017.029.03.5

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