User
Information for Reviewer
Index

JKB telah terindex di Crossref JKB telah terindex di Google Scholar JKB telah terindex di SINTA Ristekdikti JKB telah terindex di Portal Garuda

Notifications
Journal Content

Browse
Visitor Counter

 

Pengaruh Pemberian Ekstrak Propolis terhadap Ekspresi Bcl2 dan Apoptosis pada Sel Otak Tikus Model Cedera Otak Traumatik

Ully Husna, Hidayat Sujuti, Mochammad Dalhar
  Jurnal Kedokteran Brawijaya, Vol 29, No. 3 (2017),  pp.190-195  

Abstract


Ekstrak propolis mempunyai efek neuroprotektan melalui berbagai macam cara kerja salah satunya sebagai antioksidan karena bahan kandungan utamanya adalah flavonoid. Penelitian ini bertujuan untuk menguji efek ekstrak propolis berbagai dosis dalam meningkatkan ekspresi Bcl-2 dan menurunkan apoptosis sel otak tikus model cedera otak traumatik. Tikus dibagi menjadi 5 kelompok, yaitu: kelompok normal, kelompok yang diberi perlakuan cedera otak traumatis, kelompok yang diberi perlakuan cedera otak traumatis dan ekstrak propolis masing-masing dosis 50mg/kgBB, 100mg/kgBB, dan 200mg/kgBB. Setiap kelompok diambil otaknya untuk diperiksa ekspresi Bcl-2 dan apoptosis sel otak pada hari ke-7. Berdasarkan hasil analisa statistik, didapatkan hubungan signifikan antara ekspresi Bcl-2 dan apoptosis sel otak tikus model cedera otak traumatik dengan berbagai dosis ekstrak propolis (p<0,05). Dosis 200mg/kgbb merupakan dosis yang paling efektif dalam meningkatkan ekspresi Bcl2 otak tikus dan menurunkan apoptosis sel otak tikus. Penelitian ini telah membuktikan bahwa ekstrak propolis dapat meningkatkan ekspresi Bcl-2 dan menurunkan apoptosis sel otak tikus model cedera otak traumatik.

Kata Kunci: Apoptosis sel, cedera otak traumatik, ekspresi Bcl-2, ekstrak propolis


Keywords


Apoptosis sel; cedera otak traumatik; ekspresi Bcl-2; ekstrak propolis

Full Text:

PDF

References


Haddad SH and Arabi YM. Critical Care Management of Severe Traumatic Brain Injury in Adults. Scandinavian Journal of Trauma, Resuscitation, and Emergency Medicine. 2012; 20: 12

Hoh NZ, Wagner AK, and Alexander SA. BCL2 Genotypes: Functional and Neurobehavioral Outcomes after Severe Traumatic Brain Injury. Journal of Neurotrauma. 2010; 27(8): 1413-1427.

Zhang X, Chen Y, Jenkins LW, Kochanek PM, and Clark RSB. Bench-to-Bedside Review: Apoptosis/ Programmed Cell Death Triggered by Traumatic Brain Injury.Critical Care. 2005; 9(1): 66-75

Berridge MJ. Cell Stress, Inflammatory Responses and Cell Death. Cell Signalling Biology. 2012; 11: 1-29.

Khalil ML. Biological Activity of Bee Propolis in Health and Disease. Asian Pacific Journal of Cancer Prevention. 2006; 7(1): 22-31.

Halim E, Hardinsyah, Sutandyo N, Sulaeman A, Artika M, and Harahap Y. Kajian Bioaktif dan Zat Gizi Propolis Indonesia dan Brasil. Jurnal Gizi dan Pangan. 2012; 7(1): 1-6.

Wu Z, Zhu A, Takayama F, et al. Brazilian Green Propolis Supresses the Hipoxia Induced Neuroinflammatory Response by Inhibiting NF-ƙB Activation in Microglia. Oxidative Medicine and Cellular Longevity. 2013; 2013: 906726

Li S, Wu C, Zhu L, et al. By Improving Regional Cortical Blood Flow, Attenuating Mitochondrial Dysfunction and Sequential Apoptosis Galangin Acts as a Potential Neuroprotective Agent after Acute Ischemic Stroke. Molecules. 2012; 17(11): 13403-13423.

Ozkara E, Durmaz R, Kanbak G, et al. The Effect of Propolis Following Experimental Spinal Cord Injury. World Spinal Column Journal. 2014; 5(1): 6-11.

Marmarou CR, Prieto R, Taya K, Young FH, and Marmarou A. Marmarou Weight Drop Injury Model. In: Chen J (Ed). Animal Models of Acute Neurological Injuries. New York: Springer; 2007; pp. 393-407.

Johnson VJ, Stewart JE, Begbie FD, Trojanowski JQ, Smith DH, and Stewart W. Inflammation and White Matter Degeneration Persist for Years after a Single Traumatic Brain Injury. Brain. 2013; 136(1): 28–42.

Werner C and Engelhard K. Pathophysiology of Traumatic Brain Injury. British Journal of Anaesthesia. 2007; 99 (1): 4-9.

Syamsudin, Wiryowidagdo S, Simanjuntak P, and Heffen WL. Chemical Composition of Propolis from Different Region in Java and their Cytotoxic Activity. American Journal of Biochemistry and Biotechnology. 2009; 5(4): 180-183.

Brunelle JK and Letai A. Control of Mitochondrial Apoptosis by the Bcl-2 Family. Journal of Cell Science. 2009; 122(4): 437-441.

Chipuk JE, Moldoveanu T, Llambi F, Parsons MJ, and Green DR. The BCL-2 Family Reunion. Molecular Cell. 2010; 37(3): 299-310.

Shamas-Din A, Kale J, Leber B, and Andrews DW. Mechanisms of Action of Bcl-2 Family Proteins. Cold Spring Harbor Perspectives in Biology. 2013; 5(4): a008714




DOI: http://dx.doi.org/10.21776/ub.jkb.2017.029.03.3

Refbacks

  • There are currently no refbacks.