Efek Waktu Paparan Genistein terhadap Pembentukan Jantung Embrio Zebrafish
DOI:
https://doi.org/10.21776/ub.jkb.2014.028.01.5Abstract
Genistein merupakan fitoestrogen yang telah banyak diteliti manfaatnya terhadap kesehatan. Efek samping penggunaan genistein belum banyak dipelajari. Pada masa awal perkembangan paparan genistein dalam dosis tinggi dapat menyebabkan gangguan pertumbuhan dan meningkatkan angka kematian. Penelitian ini bertujuan membuktikan pengaruh perbedaan waktu paparan genistein terhadap perkembangan jantung zebrafish, melalui pengamatan pada frekuensi denyut jantung dan ketebalan pericardium dari embrio zebrafish. Embrio zebrafish dipapar dengan genistein 10 µM sejak 2, 24, dan 48 hpf (hours post fertilization). Pengamatan dilakukan pada 72 hpf pada mikroskop dengan perbesaran obyektif 40x, pengukuran ketebalan perikardium dilakukan menggunakan mikrometer pada bagian lateral embrio. Hasil penelitian ini menunjukkan bahwa paparan genistein pada tahap awal perkembangan zebrafish menyebabkan penurunan frekuensi denyut jantung dan edema perikardium. Efek tersebut akan berkurang dengan penundaan awal pemberian paparan genistein.
Kata Kunci: Denyut jantung, edema perikardium, genistein, zebrafish
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