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Pengaruh Kombinasi Artesunat Injeksi dan Ekstrak Brotowali terhadap Derajat Parasitemia serta Ekspresi HSP70 dan Endoglin pada Mencit yang Diinfeksi Plasmodium Berghei

Rahadi Swastomo, Niniek Budiarti, Loeki Enggar Fitri, Sri Poeranto
  Jurnal Kedokteran Brawijaya, Vol 29, No. 4 (2017),    

Abstract


ABSTRAK

 

Penemuan kombinasi terapi baru untuk malaria dianggap penting karena kemampuan resistensi dari Plasmodium terhadap pengobatan terdahulu. Sebanyak 18 mencit dibagi ke dalam kelompok kontrol negatif, kontrol positif, kelompok artesunat, serta  kelompok kombinasi artesunat + brotowali (50 mg, 60 mg dan 70 mg). Selain kontrol negatif, semua  mencit diinfeksi Plasmodium berghei. Selanjutnya diamati derajat parasitemianya sampai hari ke-7, kemudian dilakukan pemeriksaaan imunohistokimia guna mengetahui ekspresi HSP70 dan endoglin pada otak mencit. Kombinasi artesunat injeksi dan ekstrak brotowali terbukti dapat menurunkan derajat parasitemia lebih baik dari pemberian terapi tunggal artesunat dan meningkatkan ekspresi HSP70 dan endoglin. Kesimpulannya, kombinasi terapi malaria menggunakan artesunat dan brotowali terbukti berpotensi sebagai terapi kombinasi yang efektif terhadap malaria karena mampu menurunkan derajat parasitemia sampai 0% dan meningkatkan ekspresi HSP70 dan endoglin pada otak mencit C57BL/6J secara signifikan.

 

Kata kunci: malaria,  brotowali, artesunat, HSP70, endoglin, Plasmodium berghei.

 


Keywords


malaria, brotowali, artesunat, HSP70, endoglin, Plasmodium berghei.

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References


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