Pengaruh Pemberian Daun Ubi Jalar Ungu (Ipomoea batatas Lam) terhadap Kadar TNF-Α, Il-6 dan Nf-Κb pada Tikus yang Dipapar Asap Rokok
DOI:
https://doi.org/10.21776/ub.jkb.2015.028.03.12Abstract
Peningkatan stres oksidatif dapat memicu inflamasi yang berperan dalam perkembangan Chronic Obstructive Pulmonary Disease (COPD). Daun ubijalarungu/Purple Sweet Potato Leaves (PSPL) memiliki kandungan polifenol yang tinggi yaitu sebesar 1805mg GEA (gallic acid eguivalent) dalam 100 gram tepung PSPL. Tujuan penelitian ini untuk membuktikan pengaruh pemberian daun ketela ungu terhadap perbaikan marker inflamasi yaitu TNF-α, IL-6, dan NF-κB pada tikus yang dipapar asap rokok. Hewan coba dibagi menjadi 5 kelompok perlakuan yaitu tidak dipapar asap rokok, dipapar asap rokok, dan dipapar asap rokok dengan penambahan PSPL sebesar 0,07g, 0,14g, dan 0,28g selama 30 hari dalam bentuk tepung. Pengukuran TNF-α, IL-6, dan NF-κB dilakukan dengan menggunakan metode ELISA. Hasil penelitian menunjukkan bahwa pada tikus yang dipapar asap rokok dan ditambahkan tepung PSPL sebesar 0,14 g memiliki kadar TNF-α, IL-6, dan NF-κB paling rendah jika dibandingkan dengan kelompok yang terpapar asap rokok dengan dua dosis PSPL lainnya.
Kata Kunci: Daun ubi jalar ungu, IL-6, NF-κB, paparan asap rokok, TNF-α
Downloads
References
Obradović LN, Pešut D, Trifunović VS, and Adžić T. Influence of Tobacco Smoke on the Appearance of Oxidative Stress in Patients with Lung Cancer and Chronic Obstructive Pulmonary Diseases. Vojnosanitetski Pregled. 2006; 63(10): 893–895.
Mannino DM and Buist AS. Global Burden of COPD: Risk Factors, Prevalence, and Future Trends. The Lancet. 2007; 370(9589): 765–773.
Kode A, Yang SR, and Rahman I. Differential Effects of Cigarette Smoke on Oxidative Stress and Proinflammatory Cytokine Release in Primary Human Airway Epithelial Cells and in a Variety of Transformed Alveolar Epithelial Cells. Respiratory Research. 2006; 7(1): 132.
Lee J, Taneja V, and Vassallo R. Cigarette Smoking and Inflammation: Cellular and Molecular Mechanisms. Journal of Dental Research. 2012; 91(2):142-149.
Chang WH, Hu SP, Huang YF, Yeh TS, and Liu JF. Effect of Purple Sweet Potato Leaves Consumtion on Excercise-Induced Oxidative Stress and IL-6 and HSP72 Levels. The Journal of Applied Physiology. 2010; 109(6): 1710-1715.
Chang WH, Chen CM, Hu SP, Kan NW, Chiu CC, and Liu JF. Effect of Purple Sweet Potato Leaf Consumtion on the Modulation of the Antioxidative Status in Basketball Players during Training. Asia Pacific Journal of Clinical Nutrition. 2007; 16(3): 455-461.
Chang WH, Chen CM, Hu SP, Kan NW, Chiu CC, and Liu JF. Effect of Purple Sweet Potato Leaves Consumtion on the Modulation of the Immune Respone in Basketball Players during Training Period. Asia Pacific Journal of Clinical Nutrition. 2007; 16(4): 609-615.
Chen CM, Li SC, Lin YL, Hsu CY, Shieh MJ, and Liu JF. Consumption of Purple Sweet Potato Leaves Modulated Human Immune Respone: T- Lymphocytes Fungstion, Litic Activity of Natural Killer and Antibody Production. The World Journal of Gastroenterology. 2005; 11(37): 5777-5781.
Churg A, Dai J, Tai H, Xie C, and Wright JL. Tumor Necrosis Factor-alpha is Central to Acute Cigarette Smoke–induced Inflammation and Connective Tissue Breakdown. American Journal of Respiratory and Critical Care Medicine. 2002; 166(6): 849-854.
Demirjian L, Abboud RT, Li H, and Duronio V. Acute Effect of Cigarette Smoke on TNF-Α Release by Macrophages Mediated through the ERK1/2 Pathway. Biochimica et Biophysica Acta. 2006; 1762(6): 592-597.
Chao PY, Huang YP, and Hsieh WB. Inhibitive Effect of Purple Sweet Potato Leaf Extract and Its Components on Cell Adhesion and Inflammatory Response in Human Aortic Endothelial Cells. Cell Adhesion & Migration. 2013; 7(2): 237-245.
Wang Y, Zheng Y, Lu J, et al. Purple Sweet Potato Color Suppresses Lipopolysaccharide-Induced Acute Inflammatory Response in Mouse Brain. Neurochemistry International. 2010; 56(3): 424-430.
Kuschner WG, D'Alessandro A, Wong H, and Blanc PD. Dose-Dependent Cigarette Smoking-Related Inflammatory Responses in Healthy Adults. European Respiratory Journal. 1996; 9(10): 1989-1994.
Jeong SH, Park JH, Kim JN, et al. Up-Regulation of TNF-Alpha Secretion by Cigarette Smoke is Mediated by Egr-1 in Hacat Human Keratinocytes. Experimental Dermatology. 2010; 19(8): e206–e212.
Tseng WP, Su CM, and Tang CH. FAK Activation is Required for TNF-Alpha-Induced IL-6 Production in Myoblasts. Journal of Cellular Physiology. 2010; 223(2): 389-396.
Santangelo C, Varì R, Scazzocchio B, Di Benedetto R, Filesi C, and Masella R. Polyphenols, Intracellular Signalling and Inflammation. Annali dell'Istituto Superiore di Sanità . 2007; (43)4: 394-405.
Yordi EG, Pérez EM, Matos MJ, and Villares EU. Antioxidant and Pro-Oxidant Effects of Polyphenolic Compounds and Structure-Activity Relationship Evidence. In: Bouayed J and Bohn T. Nutrition, Well-Being and Health. Croatia: INTECH; 2012; p. 23-41.
Halliwell B. Are Polyphenols Antioxidants or Pro-Oxidants? What do We Learn from Cell Culture and In Vivo Studies? Archives of Biochemistry and Biophysics. 2008; 476(2): 107–112.
Kubo S, Kobayashi M, Masunaga Y, et al. Cytokine and Chemokine Expression in Cigarette Smoke-Induced Lung Injury in Guinea Pigs. European Respiratory Journal. 2005; 26(6): 993–1001.
Barnes PJ. The Cytokine Network in Chronic Obstructive Pulmonary Disease. American Journal of Respiratory Cell and Molecular Biology. 2009; 41(6): 631–638
Oeckinghaus A and Ghosh S. The NF-κB Family of Transcription Factor and its Regulation. Cold Spring Harbor Perspectives in Biology. 2009; 1(4); a000034.
Downloads
Published
Issue
Section
License
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
