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Pengaruh Pemberian Daun Ubi Jalar Ungu (Ipomoea batatas Lam) terhadap Kadar TNF-Α, Il-6 dan Nf-Κb pada Tikus yang Dipapar Asap Rokok

Inggita Kusumastuty, Prasetyo Adi, Leny Budhi Harti, Fajar Ari Nugroho
  Jurnal Kedokteran Brawijaya, Vol 28, No 3 (2015),  pp.228-232  

Abstract


Peningkatan stres oksidatif dapat memicu inflamasi yang berperan dalam perkembangan Chronic Obstructive Pulmonary Disease (COPD). Daun ubijalarungu/Purple Sweet Potato Leaves (PSPL) memiliki kandungan polifenol yang tinggi yaitu sebesar 1805mg GEA (gallic acid eguivalent) dalam 100 gram tepung PSPL. Tujuan penelitian ini untuk membuktikan pengaruh pemberian daun ketela ungu terhadap perbaikan marker inflamasi yaitu TNF-α, IL-6, dan NF-κB pada tikus yang dipapar asap rokok. Hewan coba dibagi menjadi 5 kelompok perlakuan yaitu tidak dipapar asap rokok, dipapar asap rokok, dan dipapar asap rokok dengan penambahan PSPL sebesar 0,07g, 0,14g, dan 0,28g selama 30 hari dalam bentuk tepung. Pengukuran TNF-α, IL-6, dan NF-κB dilakukan dengan menggunakan metode ELISA. Hasil penelitian menunjukkan bahwa pada tikus yang dipapar asap rokok dan ditambahkan tepung PSPL sebesar 0,14 g memiliki kadar TNF-α, IL-6, dan NF-κB paling rendah jika dibandingkan dengan kelompok yang terpapar asap rokok dengan dua dosis PSPL lainnya.

Kata Kunci: Daun ubi jalar ungu, IL-6, NF-κB, paparan asap rokok, TNF-α


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DOI: http://dx.doi.org/10.21776/ub.jkb.2015.028.03.12

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