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H2O2-Scavenging Activity and Hyaluronidase Inhibition Scutellarin and Apigenin in Basil Leaf Extract

Lydia Yusuf, Ermi Girsang, Ali Napiah Nasution, Cut Elvira, Satrio Haryo Benowo Wibowo, Wahyu Widowati
  Jurnal Kedokteran Brawijaya, Vol 31, No 3 (2021),  pp.133-138  


The potential utilization of flavonoid compounds, especially scutellarin, and apigenin, contained in basil (Ocimum basilicum L.) leaf extract to manage the aging effects on the skin, that occurs because of over-activated hyaluronidase enzyme and oxidative stress due to hydrogen peroxide (H2O2) radicals, is not well known. This study was conducted to assess the H2O2 scavenging activity and the inhibition of hyaluronidase from scutellarin and apigenin at various concentrations. The study was conducted by measuring H2O2 scavenging inhibition and hyaluronidase inhibition of scutellarin and apigenin in various concentrations using the spectrophotometry method. The various activity was tested using the One-Way ANOVA test followed by Tukey post hoc test. IC50 values were calculated based on linear regression equations of H2O2 scavenging inhibition and hyaluronidase inhibition. The analysis showed the highest H2O2 scavenging activity was found in scutellarin with IC50 158.76 μg/mL. Scutellarin has greater scavenging activity than apigenin. Hyaluronidase inhibition of scutellarin with IC50 35.25 µg/mL, while apigenin was 162.86 µg/mL. Scutellarin has higher hyaluronidase inhibition activity than apigenin. Antioxidant and antiaging effects of basil leaf extract caused by phytochemical compounds contained, especially scutellarin.


Antiaging; antioxidant; hyaluronidase; hydrogen peroxide; Ocimum basilicum L.

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Farage MA, Miller KW, Elsner P, and Maibach HI. Characteristics of the Aging Skin. Advances in Wound Care. 2013; 2(1): 5–10.

Prikhnenko S. Polycomponent Mesotherapy Formulations for the Treatment of Skin Aging and Improvement of Skin Quality. Clinical, Cosmetic and Investigational Dermatology. 2015; 8: 151-157.

del Valle LG. Oxidative Stress in Aging: Theoretical Outcomes and Clinical Evidences in Humans. Biomedicine & Aging Pathology. 2011; 1(1): 1–7.

Asan T, Lister IN, Fachrial E, et al. Potency of Black Soybean (Glycine Max (L.) Merr) Extract and Daidzein as Antioxidant and Antihyaluronidase. Majalah Obat Tradisional. 2019; 24(1): 52–58.

Masaki H. Role of Antioxidants in the Skin: Anti-Aging Effects. Journal of Dermatological Science. 2010; 58(2): 85–90.

Tanigawa T, Kanazawa S, Ichibori R, et al. (+)-Catechin Protects Dermal Fibroblasts Against Oxidative Stress-Induced Apoptosis. BMC Complementary and Alternative Medicine. 2014; 14: 1-7.

Ferreres F, Lopes G, Gil-Izquierdo A, et al. Phlorotannin Extracts from Fucales Characterized by HPLC-DAD-ESI-Msn: Approaches to Hyaluronidase Inhibitory Capacity and Antioxidant Properties. Marine Drugs. 2012; 10(12): 2766–2781.

Dias DA, Urban S, and Roessner U. A Historical Overview of Natural Products in Drug Discovery. Metabolites. 2012; 2(2): 303–336.

Sestili P, Ismail T, Calcabrini C, et al. The Potential Effects of Ocimum basilicum on Health: A Review of Pharmacological and Toxicological Studies. Expert Opinion on Drug Metabolism & Toxicology. 2018; 14(7): 679–692.

Utami S, Sachrowardi QR, Damayanti NA, et al. Antioxidants, Anticollagenase and Antielastase Potentials of Ethanolic Extract of Ripe Sesoot (Garcinia Picrorrhiza Miq.) Fruit as Antiaging. Journal of Herbmed Pharmacology. 2018; 7(2): 88–93.

Berim A and Gang DR. Characterization of Two Candidate Flavone 8-O-Methyltransferases Suggests the Existence of Two Potential Routes to Nevadensin in Sweet Basil. Phytochemistry. 2013; 92: 33–41.

Sestili P, Ismail T, Calcabrini C, et al. The Potential Effects of Ocimum Basilicum on Health: A Review of Pharmacological and Toxicological Studies. Expert Opinion on Drug Metabolism & Toxicology. 2018; 14(7): 679–692.

Evacuasiany E, Ratnawati H, Liana LK, et al. Cytotoxic and Antioxidant Activities of Catechins in Inhibiting the Malignancy of Breast Cancer. Oxidants and Antioxidants in Medical Science. 2014; 3(2): 141–146.

Panche AN, Diwan AD, and Chandra SR. Flavonoids: An Overview. Journal of Nutritional Science. 2016; 5: 1-15.

Rusmana D, Wahyudianingsih R, Elisabeth M, Balqis B, Maesaroh M, and Widowati W. Antioxidant Activity of Phyllanthus Niruri Extract, Rutin, and Quercetin. The Indonesian Biomedical Journal. 2017; 9(2): 84-90.

Prasain JK, Carlson SH, and Wyss JM. Flavonoids and Age-Related Disease: Risk, Benefits, and Critical Windows. Maturitas. 2010; 66(2): 163–171.

Mukhopadhyay D, Dasgupta P, Roy DS, et al. A Sensitive In Vitro Spectrophotometric Hydrogen Peroxide Scavenging Assay Using 1, 10-Phenanthroline. Free Radicals and Antioxidants. 2016; 6(1): 124–132.

Lister INE, Ginting CN, Girsang E, et al. Antioxidant Properties of Red Betel (Piper Crocatum) Leaf Extract and Its Compounds. Journal of Natural Remedies. 2019; 19(4): 198–205.

Tu PTB and Tawata S. Anti-Oxidant, Anti-Aging, and Anti-Melanogenic Properties of the Essential Oils from Two Varieties of Alpinia Zerumbet. Molecules. 2015; 20(9): 16723–16740.

Widowati W, Rani AP, Hamzah RA, et al. Antioxidant and Antiaging Assays of Hibiscus Sabdariffa Extract and Its Compounds. Natural Product Sciences. 2017; 23(3): 192–200.

Baranowska M, Suliborska K, Chrzanowski W, Kusznierewicz B, Namieśnik J, Bartoszek A. The Relationship between Standard Reduction Potentials of Catechins and Biological Activities Involved in Redox Control. Redox Biology. 2018; 17: 355–366.

Cetinkaya H, Kulak M, Karaman M, Karaman HS, and Kocer F. Flavonoid Accumulation Behavior in Response to the Abiotic Stress: Can a Uniform Mechanism be Illustrated for All Plants? (Online) 2017. flavonoids-from-biosynthesis-to-human-health/flavonoid-accumulation-behavior-in-response-to-the-abiotic-stress-can-a-uniform-mechanism-be-illustr.

Liguori I, Russo G, Curcio F, et al. Oxidative Stress, Aging, and Diseases. Clinical Interventions in Aging. 2018; 13: 757–772.

Pallardó FV, Markovic J, García JL, and Viña J. Role of Nuclear Glutathione as a Key Regulator of Cell Proliferation. Molecular Aspects of Medicine. 2009; 30(1-2): 77–85.

Hancock JT, Desikan R, and Neill SJ. Role of Reactive Oxygen Species in Cell Signalling Pathways. Biochemical Society Transactions. 2001; 29(2): 345–350.

Pittayapruek P, Meephansan J, Prapapan O, Komine M, and Ohtsuki M. Role of Matrix Metalloproteinases in Photoaging and Photocarcinogenesis. International Journal of Molecular Sciences. 2016; 17(6): 868 (1-20).

Tsai ML, Huang HP, Hsu JD, et al. Topical N-Acetylcysteine Accelerates Wound Healing In Vitro and In Vivo Via the PKC/Stat3 Pathway. International Journal of Molecular Sciences. 2014; 15(5): 7563–7578.

Shin MH, Rhie GE, Kim YK, et al. H2O2 Accumulation by Catalase Reduction Changes MAP Kinase Signaling In Aged Human Skin In Vivo. Journal of Investigative Dermatology. 2005; 125(2): 221–229.

Imokawa G and Ishida K. Biological Mechanisms Underlying the Ultraviolet Radiation-Induced Formation of Skin Wrinkling and Sagging I: Reduced Skin Elasticity, Highly Associated with Enhanced Dermal Elastase Activity, Triggers Wrinkling and Sagging. International Journal of Molecular Sciences. 2015; 16(4): 7753–7775.

Tsukahara K, Nakagawa H, Moriwaki S, Takema Y, Fujimura T, and Imokawa G. Inhibition of Ultraviolet-B-Induced Wrinkle Formation by an Elastase-Inhibiting Herbal Extract: Implication for the Mechanism Underlying Elastase-Associated Wrinkles. International Journal of Dermatology. 2006; 45(4): 460–468.

Widowati W, Fauziah N, Herdiman H, et al. Antioxidant and Anti-Aging Assays of Oryza sativa Extracts, Vanillin and Coumaric Acid. Journal of Natural Remedies. 2016; 16(3): 88–99.

Ebanks JP, Wickett RR, and Boissy RE. Mechanisms Regulating Skin Pigmentation: The Rise and Fall of Complexion Coloration. International Journal of Molecular Sciences. 2009; 10(9): 4066–4087.

Park KT, Kim JK, Hwang D, Yoo Y, and Lim YH. Inhibitory Effect of Mulberroside A and Its Derivatives on Melanogenesis Induced by Ultraviolet B Irradiation. Food and Chemical Toxicology. 2011; 49(12): 3038–3045.

Hamid MA, Sarmidi MR, and Park CS. Mangosteen Leaf Extract Increases Melanogenesis In B16F1 Melanoma Cells by Stimulating Tyrosinase Activity In Vitro And By Up-Regulating Tyrosinase Gene Expression. International Journal of Molecular Medicine. 2012; 29(2): 209–217.

Goldman A. Hyaluronic Acid Dermal Fillers: Safety and Efficacy for the Treatment of Wrinkles, Aging Skin, Body Sculpturing and Medical Conditions. Clinical Medicine Reviews in Therapeutics. 2011; 3: 107-121.

Hertel W, Peschel G, Ozegowski JH, and Müller PJ. Inhibitory Effects of Triterpenes and Flavonoids on the Enzymatic Activity of Hyaluronic Acid-Splitting Enzymes. Archiv der Pharmazie. 2006; 339(6): 313–318.

Zeng HJ, Ma J, Yang R, Jing Y, and Qu LB. Molecular Interactions of Flavonoids to Hyaluronidase: Insights from Spectroscopic and Molecular Modeling Studies. Journal of Fluorescence. 2015; 25(4): 941–959.



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