PENINGKATAN KADAR LEPTIN DENGAN PUFA (Polyunsaturated Fatty Acid) MELALUI AKTIVASI PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR gamma (PPARγ) PADA KULTUR PREADIPOSIT KELINCI
DOI:
https://doi.org/10.21776/ub.jkb.2005.021.02.4Abstract
ABSTRACT For the past three decades, PUFA has been recognized as importnant energy sources and cell membrane component. PUFA also plays key roles in many cellular events such as gene regulation and leptin activation. The role of this fatty acid is still unknown especially in vitro. The purpose of this research was to examine the effect of PUFA of the corn oil in increasing leptin concentration by PPARγ activation. Preadipocyte culture from rabbit was incubated by PUFA of corn oil. PUFA have been given on doses 0, 4, 8 and 16 µM. PPARγ protein and leptin levels were analyzed by ELISA. PPARγ expression  was  analyzed  by  immunocytochemistry.  The research  result  showed  that  every  treatment  has  increased PPARγ levels inconsistently. PPARγ concentration increased significantly on 4 µM PUFA treatment compared to control, 8 and 16 µM PUFA of corn oil. But there were no significant difference between control and 8 µM PUFA treatments. Leptin levels increased significantly between administration on 4 µM PUFA and 16 µM PUFA of corn oil administration. Leptin levels was not significantly different between control and 8 µM PUFA. There was also strong correlation between PPARγ expression and leptin levels. PUFA of corn oil increases leptin levels through PPARγ activation. Key words: PUFA, preadipocyte, leptin, PPARγDownloads
References
DAFTAR KEPUSTAKAAN
Steven DC. Polyunsaturated Fatty Acid Regulation of Gene Transcription: A Molecular Mechanism to Improve the Metabolic Syndrome. American Society for Nutritional Sciences 2001.
Yasuki K, Satoshi M, Keiji E, Hiroshi S, and Tohru K. Regulation of Insulin-like Growth Factor-I Expression in Mouse Preadipocyte Ob1771 Cells. 1996; 271(17): 9883-9886
Maffei M, Halaas J and Ravussin E. Leptin Levels in Humans and Rodent: Measurement of Plasma Leptin and ob RNA
in Obese and Weight Reduced Subjects. Nat. Med 1995; 1:1155-1161
MacDouglas OA, Hwang CS and Fan H. Regulated Expression of The Obese Gene Product (Leptin) in White Adipose Tissue and 3T3-L1 Adipocytes. USA: Proc. Natl. Acad. Sci; 1995; 92: 9034-9037.
Caro J, Sinha M and Kolaczynski J. Leptin The Tale of an Obesity Gene. Diabetes 1996; 45:1455-1462
Kolaczynski H, Ogawa Y and Hosoda K. Augmented Expression of The Obese Gene in The Adipose Tissue From Rat Fed High-Fat Diet. Biophys. Res. Commun. Biochem 2001; 216: 355-358
Fried S K and Moussa N M. Culture of adipose Tissue and Isolated Adipocytes.Methods in Molecular Biology Adipose
Tissue Protocols. Alhaud G (Ed.)Totowa, New Jersey: Human Press; 2001; 197-212.
Lombardo YB, Drago S, Chicco A, Fainstein-Day P Gagliardino JJ and Gomez-Dumm CL. Long Term Administration of Sucrose-Rich Diet to Normal Rats: Relationship between Metabolic and Hormonal Profiles and Morphological Changes
in The Endocrine Pancreas. Metabolism 2002; 45: 1527-1532
Clarke SD. Polyunsaturated Fatty Acid Regulation of Gene Transcription: a Mechanism to Improve Energy Balance and
Insulin Resistance. Br. J. Nutr 2000; 83: S59-S66
Brown V. Clinical Interest of Ppars Ligands. Particular Benefit in Type 2 Diabetes and Metabolic Syndrome Diabetes
Metab. 2004; 30; 7-12
Bidlingmaler M and Mathias T. Immunoassays Measurement of Leptin in Molecular Biology Adipose Tissue Protocols.
Alhaud G (Ed.)Totowa, New Jersey: Human Press; 2001; 323-330.
Peyron-Caso E, Fluteau-Nadler S, Kabir M, Guerre-Millo, Quignard-Boulange A, Slama G and Rizkalla SW. Regulation
of Glucose Transport and Transporter-4 (GLUT-4) in Muscle and Adipocyte of Sucrose-Fed Rats: Effects of N3 Poly And Monounsaturated Fatty Acids. Horm. Metab. Res. (in press) 2002.
Zuo X, Wu Y, Morris JS, Stimmel JB, Leesnitzer LM, Fischer SM, Lippman SM, and Shureiqi I. Oxidative Metabolism of Linoleic Acid Modulates PPAR-Beta / Delta Suppression of PPAR-Gamma Activity. Oncogene advance online publication,14 November 2005.
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