Distribusi M. Tuberculosis Genotipe Beijing pada Pasien Tuberkulosis Paru di Malang

Authors

  • Tri Yudani MR Laboratorium Biokimia - Biologi Molekuler Fakultas Kedokteran Universitas Brawijaya Malang
  • Triwahju Astuti Bagian Paru Fakultas Kedokteran Universitas Brawijaya / Rumah Sakit Umum Dr. Saiful Anwar Malang

DOI:

https://doi.org/10.21776/ub.jkb.2010.026.02.3

Abstract

ABSTRAK
Penelusuran  penyebaran  genetis  Mycobacterium  tuberculosis  merupakan  salah  satu  faktor  penting  dalam
mengendalikan infeksi Mycobacterium tuberculosis. Studi mengenai distribusi Mycobacterium tuberculosis galur Beijing
pada TB paru BTA (+) pasien di RSU dr. Saiful Anwar rumah sakit ini   dilaksanakan dengan metode spoligotyping. Dahak
dikumpulkan dari total 41 pasien, dicuci dengan salin normal dan ditanam pada medium LJ. DNA dari bakteri yang tumbuh
kemudian diisolasi dan mengalami spoligotyping. Sembilan belas spoligotype (65,5%) ditemukan dari total dua puluh
sembilan sampel yang berkembang, sedangkan sepuluh sampel (34,5%) adalah non-Mycobacterium tuberculosis. Dari
spoligotype sembilan belas, sembilan sampel merupakan Beijing strain (31%) dan sepuluh sampel (34,5%) menunjukkan
strain non Beijing. Proporsi pasien TB yang terinfeksi, menunjukkan bahwa persentase pasien terinfeksi dengan strain
Beijing hampir sama antara wanita dan pria. Ditemukan bahwa prevalensi tertinggi strain Beijing pada pasien dengan usia
berkisar antara 31-40 tahun (51%). Oleh karena itu dapat disimpulkan bahwa strain Beijing mendominasi distribusi M.
tuberculosis pada pasien TB paru di Malang.
Kata Kunci: Beijing strain, M. tuberculosis, Spoligotyping

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Published

2013-03-10

Issue

Vol. 26 No. 2 (2010)

Section

Research Article

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