Pentagamavunon‒0 menurunkan Ekspresi Cyclooxygenase 2 dan Jumlah Sel Epitel Luminal Endometrium Tikus yang Diinduksi dengan Luteinizing Hormone

Authors

  • Soraya - Rahmanisa Fakultas Kedokteran Universitas Lampung
  • Muhartono Sudarmo Kasban Fakultas Kedokteran Universitas Lampung

DOI:

https://doi.org/10.21776/ub.jkb.2015.028.04.2

Abstract

Pentagamavunon‒0 (PGV‒0) atau 2,5‒bis‒(4'‒hidroksi‒3'‒metoksi‒benzilidin siklopentanon merupakan senyawa analog kurkumin dengan perubahan struktur pada gugus β diketon menjadi gugus monoketon. Aktivitas dan mekanisme PGV‒0 pada sistem reproduksi khususnya terhadap ekspresi Cyclooxygenase 2(COX‒2) dan ketebalan endometrium belum dapat dijelaskan. Serbuk PGV‒0 dilaporkan mempunyai aktivitas biologis lebih baik daripada senyawa asalnya. Tujuan penelitian ini adalah untuk mengkaji pengaruh PGV‒0 terhadap ekspresi COX‒2, ketebalan endometrium, dan jumlah sel epitel luminal endometrium Rattus norvegicus yang diinduksi dengan Luteinizing Hormone (LH). Subjek penelitian ini adalah 30 ekor tikus putih betina Sprague dawley umur 28 hari setelah diinduksi dengan pregnant mare's serum gonadotropin. Setelah semua tikus ovulasi, tikus kemudian diberikan PGV‒0 dosis 55,2mg/kgBB selama 4 hari setelah diinduksi dengan LH pada hari pertama perlakuan. Pada kelompok kontrol diberikan senyawa pelarut PGV‒0 yaitu Carboxyl Metil Cellulose (CMC). Pada umur 34 hari hewan coba dikorbankan untuk diambil uterusnya dan dibuat sediaan histologi dan imunohistopatologik. Hasil penelitian menunjukkan pemberian PGV‒0 dosis 55,2mg/kgBB berpengaruh nyata menurunkan ekspresi COX‒2 (p<0,05) dan jumlah sel epitel luminal endometrium (p<0,05) tetapi tidak berpengaruh nyata terhadap penebalan endometrium tikus yang diinduksi dengan LH (p<0,05).Kata Kunci: COX‒2, ketebalan endometrium, LH, PGV‒0

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Author Biographies

Soraya - Rahmanisa, Fakultas Kedokteran Universitas Lampung

Bagian Biologi Medik Fakultas Kedokteran Universitas Lampung

Muhartono Sudarmo Kasban, Fakultas Kedokteran Universitas Lampung

Bagian Patologi Anatomi FK Unila

References

Cunha GR, Cooke PS, and Kurita T. Role of Stromal-Epithelial Interactions in Hormonal Responses. Archive of Histology Cytology. 2004; 67(5): 417-434.

Strowitzki T, Germeyer A, Popovici R, and Wolff MV. The Human Endometrium as a Fertility-Determining Factor. Human Reproduction Update. 2006; 12(5): 617-630.

Murdoch WJ, Hansen TR, and McPherson LA. A Review—Role of Eicosanoids in Vertebrate Ovulation. Prostaglandins. 1993; 46(2): 85–115.

Marions L and Danielsson KG. Expression of Cyclooxygenase in Human Endometrium during the Implantation Period. Molecular Human Reproduction. 1999; 5(10): 961–965

Sirois J, Sayasith K, Brown KA, Stock AE, Bouchard N, and Dore M. Cyclooxygenase‒2 and Its Role in Ovulation: A 2004 Account. Human Reproduction Update. 2004; 10(5): 373‒385.

Matsuzaki S, Canis M, Pouly JL, Wattiez A, Okamura K, and Mage G. Cyclooxygenase-2 Expression in Deep Endometriosis and Matched Eutopic Endometrium. Fertility and Sterility. 2004; 82(5): 1309-1315.

Smith WL, Garavito RM, and DeWitt DL. Prostaglandin Endoperoxide H‒ Synthases‒1 and ‒2. The Journal of Biological Chemistry. 1996; 271(52): 33157-33160.

Shemesh M, Mizrachi D, Gurevich M, Stram Y, Shore LS, and Fields MJ. Direct Involvement of Luteinizing Hormone in Uterine Function. Reproduction Domestical Animals. 2000; 35(3-4): 163‒166.

Kim JJ, Wang J, Bambra C, Das SK, Dey SK, and Fazleabas AT. Expression of Cyclooxygenase‒1 and ‒2 in the Baboon Endometrium during the Menstrual Cycle and Pregnancy. Journal of Endocrinology. 1999; 140(6): 2672‒2678.

Shemesh M, Freidman S, HarelME, and Gurevich M. Induction and Regulation of the Cyclooxygenase in the Bovine Endometrium. Di dalam: Zor M (Ed). Lipid Mediators in Health and Disease. Tel Aviv: Freund Publishing House; 1994: pp. 71-78.

Shemesh M. Actions of Gonadotrophins on the Uterus (Review). Reproduction. 2001; 121(6): 835‒842.

Sardjiman. Sintesa 2,6‒Bis (3,5‒Dimetil‒4‒Hidroksi Benzilidin) Sikloheksanon, 2,5‒(3,5‒dimetil‒4-Hidroksi Benzilidin) Siklopentanon & Pentadien‒on dan Daya antioksidannya. [Laporan Penelitian]. Universitas Gadjah Mada, Yogyakarta. 1993.

Sardjiman dan Samhoedi MR. Synthesis of Some New Series of Curcumin, Analogues, Antioxydative, Antiinflammatory, Antibacterial Activities and Qualitative Structure Activity Relationships. [Disertasi]. Universitas Gadjah Mada, Yogyakarta. 2000.

Hakim L. Pharmacokinetics and Biological Activities of a Curcumin Analog‒Pentagamavunon‒0. Proceeding of The International Symposium on Recent Progress in Curcumin Research. Yogyakarta, 2007; hal. 37‒55.

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Published

2015-08-28

Issue

Vol. 28 No. 4 (2015)

Section

Research Article

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