PROTEIN FIMBRIA 16 kDa BAKTERI ACINETOBACTER BAUMANNIIDARI URIN PENDERITA INFEKSI SALURAN KEMIH BERPERAN SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN

Authors

  • Noorhamdani Noorhamdani Laboratorium Mikrobiologi Fakultas Kedokteran Unibraw / RSU dr Saiful Anwar Malang

DOI:

https://doi.org/10.21776/ub.jkb.2005.021.01.8

Abstract

Acinetobacter baumannii (Acb) is an opportunistic and nosocomial pathogen that ussualy found in clinical specimen from patients with intensive care. The pathogenic mechanism of this bacteria are not fully elucidated especially potential activity of its protein as hemaglutinin and adhesion molecul. The aim of this study is to evaluate the  role of 16 kDa fimbriae protein from urnary tract infection (UTI) patient as hemaglutinin and adhesion molecule. Usingexplorative design this study was started by isolation Acb bacteria from urine of patient that had been determine as UTI clinically and laboratory. After identification this bacteria by microbact system hemaglutination test and isolation  of its fimbriae fraction, 12.5% SDS-PAGE had been used to isolated fimbriae protein, following assay in vitro to adhesion test..The study showed that the 16 kDa fimbriae protein of Acb bacteria was a hemaglutinin protein that could agglutinate 0.5% mice erythrocytes (1/32), and human blood group O erythrocytes (1/8). Hemaglutination test were negative onerythrocytes from rat, guinea pig, sheep, and human blood group A, B. The 16 kDa fimbriae protein (AF16) was also adhesion protein that had been revealed by its activity to adherenceto receptor of mice enterocytes. The increasing dose of AF16 molecules will decrease the amount of Acb bacteria to adherence to enterocytes (p<0.05). The fimbriae of Acb is maybe classified P type. Key words:Acinetobacter baumannii, fimbriae, hemaglutinin protein, adhesion protein

Downloads

Download data is not yet available.

References

Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST. Bergey's Manual of Determinative of Bacteriology, 9th. Baltimor: William & Wilkins; 1994.

Seifert H, Dijkshoorn L, Gerner-Smidt P, Pelzer N, Tjernberg I and Vaneechoutte. Distribution of Acinetobacter species on Human skin: Comparison of Phenotypic and Genotypic Identification Methods. J Clin Microbiol 1997;35:2819-2825.

Holmes B and Howard BJ. Nonfermentative Gram-Negative Bacteria. In: Clinical and Pathogenic Microbiology, 2nd ed. Howard BJ et al (ed.), St Louis, Washington DC, Toronto: CV Mosby Co; 1994;337-364.

Gould IM, MacKenzie F, Thomson C. Acinetobacter Infections, Intensive Care Units, andHandwashing (letter; comment). Lancet 1995; 345: 122.

Humpreys H, Towner KJ, Crowe M, Webster C, Winter R. Acinetobacter Infections, Intensive Care Units, Handwashing (letter). Lancet 1995; 345:121.

Scerpella EG, Wanger AR, Armitige L, Anderlini P, Ericsson CD. Nosocomial Outbreak Caused by a Multiresistent Clone of: Results of Case Control and Molecular Epidemiologic Investigations. Infect Control Hosp Epidemiol 1995;16:92-97.

Bilgic H, Akin ES, Tasan Y, Ekiz K, Seber O. A case of Acinetobacter calcoaceticus Pneumonia. Thorax 1995;50:315-316.

Khater TT, Jones DB, Wilhelmus KR. Infectious Crystalline Keratopathy Caused by Gram-Negative Bacteria. American J Ophthalmology 1997; 124: 19-23.

Kirwan JF, Potamitis T, el-Kasaby H, Hope-Ross Mw, Sutton GA. Microbial Keratitis in Intensive Care. B Med J 1997;314:433-534.

Josodiwondo S. Perbandingan Pola Kepekaan Kuman Isolat Urin Asal Rumah Sakit dan Non-Rumah Sakit Pada Triwulan I Tahun 1994. Jurnal PAMKI 1994; 7 (1): 7-13, Desember.

Dzen SM. Multiresisten Acinetobacter anitratus yang di Isolasi dari Bahan Pemeriksaan Klinik. Jurnal PAMKI 1996; 7:2.

Noorhamdani. Pola Kuman Isolat Biakan Urin dan PolaResistensinya Terhadap Antibiotika di RSUD dr Saiful Anwar Malang. Majalah Kedokteran Universitas Brawijaya 1995;11: 43-52.

Noorhamdani. Isolat Acinetobacter baumannii dari Biakan Urin danTes Kepekaannya Terhadap beberapa Antibiotik. Majalah Kedokteran Universitas Brawijaya 2001; 17: 55-58

Kasminem. Pola Kuman Isolat Penyebab Infeksi Saluran Kemih dan Pola Resistensinya Terhadap Antibiotika di RSUD dr Saiful Anwar Malang. Karya Tulis Sebagai Tugas Akhir, Depkes RI, Politeknik Kesehatan Surabaya Jurusan Analis Kesehatan, 2002.

Noorhamdani. Aktivitas Hemaglutinasi Bakteri Acinetobacter baumannii yang Berasal dari Spesimen Klinik dan Lingkungan. Jurnal Kedokteran Brawijaya (JKB) 2004; 20:2:105-109.

Santoso S. Protein Adhesin Salmonella typhi Sebagai Faktor Virulensi Berpotensi Imunogenik Terhadap Produksi S-IgA Protektif. [Disertasi] Surabaya: Program Pasca Sarjana Universitas Airlangga, 2002.

Sumarno. Karakterisasi Molekuler Protein Adesi Vibrio cholerae 01 M094V dan Protein Reseptornya pada Sel Epitel Usus Halus Tikus Putih (Wistar).Studi Patogenesis Vibrio cholerae01 M094V. [Disertasi]. Surabaya: Program Pasca Sarjana Universitas Airlangga, 2000.

Kimura A, Mountzouros KT, Relman DA, Falkow S, CowellJL. Bordetella pertussis Filamentous Hemaglutinin:

Evaluation as a Protective Antigen and ColonizationFactor in a Mouse Respiratory Infection Model. Infect Immun 1990;58:7-16.

Salyers AA and Whitt DD. Bacterial Pathogenesis A Molecular Approach. Washington DC: ASM Press; 1994.

Salyers AA and Whitt DD. Bacterial Pathogenesis A Molecular Approach.Washington DC: ASM Press; 2002.

Campos JM, McNamara AM and Howard BJ. Specimen collection and Processing. In: Clinical and Pathogenic Microbiology, 2nd ed. Howard BJ et al (eds.), St Louis, Washington DC, Toronto: CV Mosby Co.; 1994; 213-242.

Arthur M, Johnson CE, Rubin RH, Rubin RH, Arbeit RD, Campanelli C, Kim C, Steinbach S, Agarwal M, Wilkinson R, and Goldstein R. Molecular Epidemiology of Adhesin and Hemolysin Virulence Factors among Uropathogenic Escherichia coli. Infect Immun 1989; 57: 303-313.

Microbact System 24E for the Identification of Miscellaneous Gram Negative Bacilli, (tanpa tahun). 3-rd ed. Adelaide, South Australia: Disposible Products Pty.Ltd.

McGarey DJ and Allred DR. Characterization of Hemaglutinating Components on the Anaplasma marginale. Initial Body Surface and Identification of Possible Adhesins. Infect Immun 1994; 62: 4587-4593.

Ehara M, Ishibashi M, Ichinose Y, Iwanaga M, Shimotori S, Naito T. Purification and Partial Characterization of Fimbriae of Vibrio cholerae 01. Vaccin 1987; 5: 283 – 286.

Laemmli UK. Cleavage of Structural Protein during the Assembly of the Head of Bacteriophage T4. Nature 1970;227:680-686.

Nagayama K, Oguchi T, Arita M, Honda T. Purification and Characterizations of a Cell-Associated Hemaglutinin of Vibrio parahaemolyticus. Infect Immun 1995; 63: 1987-1992.

Becker JM, Caldwell GA and Zachgo EA. Biotechnology a Laboratory Course. San Diego, New York: Academic Press Inc.;1990; 193-194.

Martino PD, Bertin Y, Girardeau JP, Livrelli V, Joly B, Darfeuille-Michaud A. Molecular Characterization and Adhesive Properties of CF29K, an Adhesin of Klebsiella pneumoniae Strain Involved in Nosocomial Infection. Infect Immun 1995; 63: 4336-4344.

Winarsih S, Sumarno dan Roekistiningsih. Kajian Fungsi dan Sifat Immunogenitas Protein Hemaglutinin 32kD dan 20kD pada Helicobacter pylori.Majalah Kedokteran Universitas Brawijaya 1997; 13:135-141.

Musa MS dan Nasution AH. Perancangan dan Analisa Percobaan Ilmiah. Depdikbud-Dirjen Dikbud, PAU-IPB; 1989.

Brooks GF, Butel JS, and Morse SA. Jawetz, Melnick and Adelberg’s Medical Microbiology, 21th ed. Stamford, Connecticut: Appleton & Lange; 1998.

Schreckenberger PC and von Graevenitz A. Acinetobacter, Achromobacter, Alcaligenes, Moraxella, Methylobacterium, and other Nonfermentative Gram-Negative Rods. In: Manual of Clinical Micrbiology, Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH (eds.). Washington DC: ASM Press;1999; 539-543.

Progulske-Fox A, Tumwasorn S and Holt S. The Expression and Function of a Bacteroides gingivalis Hemaglutinin gene in Escherichia coli.Oral Microbiol Immunol 1989; 4: 11-131.

Hechemy KE, Samsonoff WA, Harris HL and McKee M. Adherence and Entry of Borrelia burgdoferi in Vero cells. J Med Microbiol 1992; 36: 229-238.

Chmiela M, Ringner M andWadstrom T. Detection of Cell Surface Haemaglutinins of Helicobacter pylori. In:Helicobacter pylori: Techniques for Clinical Diagnosis and Basic Research. Lee A, and Megraud F, (eds.). London, Philadelphia, Toronto, Sidney, Tokyo: WB Saunders Co.; 1996;213-223.

Alam M, Miyoshi S, Tomochika K, Shinoda S. Vibrio mimicus Attaches to The Intestinal Mucosa byOuter Membran Hemaglutinin Specific to Polypeptide Moities of Glycoprotein. Infect Immun 1997; 65: 3662-3665.

Evans DG, Evans DJ, Moulds JJ, Graham DY. N-Acetyl- neuraminyllactose-Binding Fibrillar Hemagglutinin of Campylobacter pylori: a Putative Colonization FactorAntigen. Infect Immun 1988; 56: 2896-2906.

O’Hanley P. Prospect for Urinary Tract Infection Vaccines. In: Urinary Tract Infections, Molecular Pathogenesis and Clinical Management.Mobley HLT and Warren JW (eds.), Washington DC: ASMPress; 1996; 417.

Nakazawa T, Ishibashi M, Konishi H, Takemoto T, Shigeeda M and Kochiyama T. Hemaglutination Activity of Campylobacter pylori.Infect Immun 1989; 57: 989-991.

Robertson MS, Cade JF, Savoia HF and Clancy RL. Helicobacter pylori Infection in the Australian Community: Current Prevalence and Lack of Association with ABO Nlood Group. Intern Med J. 2003; 33 (4): 163-167.

Shankar SG, Rajjith MS, Ranganathan S, Sivaramakhrisnana M, Natarajan V and Rasool SK. Role of Blood Groups in the Infection rate of Dandruff Caused by Pityrosporum ovale.Indian J Dermatol 2002; 47 (1): 21-23.

Hutson AM, Atmar RL, Graham DY and Estes MK. Norwalk Virus Infection and Disease is Associated with ABO Histo Blood Group Type.J Infect Dis 2002; 185 (9): 1335-1337.

Soto GE and Hultgren SJ. Bacterial Adhesins: Common Themes and Variations in Architecture and Assembly (Minireview).Infect Immun 1999; 181: 1059-1071.

Barza M. Urinary Tract. In: Mechanisms of Microbial Disease, 2nd ed., Schaechter M, Medoff G and Eisenstein BI(eds.).Baltimore: William and Wilkins;1993;891–898.

Downloads

Published

2013-04-18

Issue

Vol. 21 No. 1 (2005)

Section

Research Article

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).