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Minyak Cengkeh (Syzygium aromaticum) Menginduksi Apoptosis pada Sel Kanker Servik HeLa melalui Peningkatan Kadar Protein p53

Happy Kurnia Permatasari, Ihda Dian Kusuma, Elly Mayangsari
  Jurnal Kedokteran Brawijaya, Vol 30, No 3 (2019),  pp.185-190  

Abstract


Kanker serviks merupakan kanker yang paling sering dijumpai pada wanita setelah kanker payudara. Kanker ini terkait dengan infeksi persisten virus yaitu Human Papillomovirus (HPV). Virus ini mengekspresikan protein onkogenik virus yaitu protein E6 dan E7 yang terkait dengan proses karsinogenesis. Salah satu mekanisme onkogenik virus ini adalah pengikatan protein p53 yang menginduksi degradasi oleh protein E6, mengakibatkan efek anti-apoptosis dan proliferasi sel secara terus menerus. Minyak cengkeh yang mengandung senyawa aktif eugenol telah dilaporkan memiliki efek anti kanker pada beberapa kanker. Namun, mekanisme minyak cengkeh yang terkait dengan penghambatan kanker servik masih belum jelas. Penelitian ini, untuk mengkaji efek minyak cengkeh dari Syzygium aromaticum dalam efek pro-apoptosis sel kanker servik HeLa terkait dengan kadar protein p53. Tingkat apoptosis diamati dengan pewarnaan dengan Annexin-V dan PI dan dilakukan dengan metode flow-cytometry. Dilakukan pengecatan immunohistokima untuk melihat ekspresi caspase-3 aktif untuk mengonfirmasi sel yang apoptosis, sedangkan kadar protein p53 dievaluasi dari lisat sel menggunakan ELISA protein p53. Hasil studi menunjukkan bahwa minyak cengkeh memiliki efek pro-apoptosis, berkaitan dengan kadar protein p53, pada sel kanker serviks secara in vitro.


Keywords


Apoptosis; minyak cengkeh; sel HeLa; Syzigium aromaticum

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References


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DOI: http://dx.doi.org/10.21776/ub.jkb.2019.030.03.4

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