Ekstrak Propolis Lokal Mempunyai Efek Sitotoksik dan Antiproliferatif Terhadap Sel HeLa

Authors

  • Zauhani Kusnul Hadiyah Akademi Keperawatan Bahrul Ulum Jombang
  • Sri Widyarti Laboratorium Biologi Molekuler Fakultas MIPA/Biologi Universitas Brawijaya
  • Muhamad Aris Widodo Laboratorium Farmakologi Fakultas Kedokteran Universitas Brawijaya

DOI:

https://doi.org/10.21776/ub.jkb.2009.025.01.4

Abstract

ABSTRACT
Propolis is resin with complex chemical composition that collected by honeybees from various plants. It has
been known that propolis has several biological properties, such as antimicrobial, anti-inflammatory,
antioxidant and immunomodulatory activities. The aim of this study is to evaluate the propolis's cytotoxic and
antiproliferative activity in vitro on hela cell line. These cells were incubated with different concentrations of
propolis (5, 10, 25, 50, and 100 µg/ml) for different time periods (6, 24, dan 48 hours), and the number of viable
and died cells were analyzed by trypan blue dye exclution and were counted using hemocytometer. The
result of this study showed that the IC50 of local propolis against HeLa cell line is 27,725 µg/ml. It can be
concluded that local propolis extract exhibited a cytotoxic and antiproliferatif effect in vitro against HeLa cell
line, in a dose- and time dependent.
Key words: propolis, cytotoxic, antiproliferative, HeLa cell line

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References

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Issue

Vol. 25 No. 1 (2009)

Section

Research Article

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