Analisis Konsentrasi Low Density Lipoprotein Teroksidasi Serum pada Tahapan Aterosklerosis
DOI:
https://doi.org/10.21776/ub.jkb.2017.029.04.11Keywords:
Aterosklerosis, oxLDL, stres oksidatifAbstract
Aterosklerosis merupakan proses yang terjadi secara bertahap meliputi tahap inisiasi, progresi dan komplikasi. Beberapa penelitian memperlihatkan kaitan antara stres oksidatif dengan aterosklerosis, tetapi belum ada penelitian yang menganalisis perbedaan stres oksidatif pada berbagai tahap aterosklerosis. Konsentrasi oxidized low density lipoprotein (oxLDL) menggambarkan stres oksidatif yang terjadi sehingga dikenal sebagai marker stres oksidatif. Penelitian ini bertujuan untuk menganalisis konsentrasi oxLDL serum pada berbagai tahap aterosklerosis pada hewan coba. Pada penelitian ini dilakukan pengukuran kadar oxLDL serum pada 24 ekor tikus yang terdiri dari kelompok normal, inisiasi, progresi dan komplikasi. Induksi aterosklerosis dilakukan dengan pemberian vitamin D3 (700.000 IU/kg) dan pakan tinggi lipid (kolesterol 2%, lemak kambing 5%, asam kolat 0,2%) masing-masing selama 2 hari, 4 hari dan 2 minggu. Konsentrasi oxLDL serum diukur dengan teknik Enzyme linked immunosorbent assays (ELISA). Data disajikan sebagai rata-rata±SD. Uji statistik yang digunakan adalah uji Anova. Konsentrasi oxLDL serum lebih tinggi pada inisiasi dibandingkan normal (2,17±0,26ng/mL versus 1,93±0,25ng/mL, p>0,05) dan lebih tinggi lagi pada progresi dan komplikasi (2,75±0,25ng/mL dan 3,3±0,61ng/mL, p<0,05) masing-masing terhadap normal dan inisiasi). Penelitian ini menyimpulkan bahwa terdapat perbedaan konsentrasi oxLDL pada berbagai tahap aterosklerosis dan konsentrasi paling tinggi pada tahap komplikasi. Hal ini membuka kemungkinan untuk mengembangkan oxLDL sebagai marker tahapan aterosklerosis.
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References
Alsheikh-Ali AA, Kitsios GD, Balk EM, Lau J, and Ip S. The Vulnerable Atherosclerotic Plaque: Scope of the Literature. Annals of Internal Medicine. 2010; 153(6): 387-395.
Srinivasa-Rao PVLN and Kiranmayi VS. Biochemical Mechanism Underlying Atherogenesis. Journal of Clinical and Scientific Research. 2012; 1:24-34.
Mizuno Y, Jacob RF, and Mason RP. Inflammation and the Development of Atherosclerosis. Journal of Atherosclerosis and Thrombosis. 2011; 18(5): 351-358.
Parthasarathy S. Atherogenesis. 1st edition. Rijeka: Intech; 2012; p. 187-202.
Insull W. The Pathology of Atherosclerosis: Plaque Development and Plaque Responses to Medical Treatment. The American Journal of Medicine. 2009; 122(1): S3-S14.
Vogiatzi G, Tousoulis D, and Stefanadis C. The Role of Oxidative Stress in Atherosclerosis. Hellenic Journal of Cardiology. 2009; 50(5): 402-409.
Halliwell B and Gutteridge JMC. Free Radicals in Biology and Medicine. 4th edition. New York: Oxford University Press; 2007; pp. 625-638.
Murray RK, Bender DA, Botham KM, Kennelly PJ, Rodwell VW, and Weil PA. Harper's Illustrated Biochemistry. 26th edition. USA: McGraw-Hill Companies; 2012; pp. 80-236.
Gueraud F, Atalay M, et al. Chemistry and Biochemistry of Lipid Peroxidation Products. Free Radical Research. 2010; 44(10): 1098-1124.
Ismawati, Asni E, dan Hamidy MY. Pengaruh Air Perasan Umbi Bawang Merah (Allium ascalonicumL.) terhadap Malondialdehid (MDA) Plasma Mencit yang diinduksi Hiperkolesterolemia. Jurnal Natur Indonesia. 2012; 14(2): 150-154.
Li J, Chen CX and Shen YH. Effects of Total Glucosides from Paeony (Paeonialactiflora Pall) Roots on Experimental Atherosclerosis in Rats. Journal of Ethnopharmacology. 2011; 135(2): 469-475.
Pang J, Xu Q, Xu X, et al. Hexarelin Suppresses High Lipid Diet and Vitamin D3-Induced Atherosclerosis in the Rat. Peptides. 2010; 31(4): 630-638.
Lukitasari N, Ratnawati R, dan Lyrawati D. Polifenol Buah Tin (Ficus carica Linn) Menghambat Peningkatan Kadar MCP-1 pada Tikus dengan Diet Tinggi Lemak. Jurnal Kedokteran Brawijaya. 2014; 8(1): 1-5.
Ismawati, Oenzil F, Yanwirasti, and Yerizel E. Changes in Expression of Proteasome in Rats at Different Stages of Atherosclerosis. Anatomy & Cell Biology. 2016; 49(2): 99-106.
Jiang F, Qian J, Chen S, Zhang W, and Liu C. Ligustrazine Improves Atherosclerosis in Rat via Attenuation of Oxidative Stress. Pharmaceutical Biology. 2011; 49(8): 856–863.
Getz GS and Reardon CA. Animal Models of Atherosclerosis. Arteriosclerosis, Thrombosis and Vascular Biology. 2012; 32(5): 1104-1115.
Jawien J. The Role of An Experimental Model of Atherosclerosis: apoE-knockout Mice in Developing New Drugs against Atherogenesis. Current Pharmaceutical Biotechnology. 2012; 13(13): 2435-2439.
Verhoye E and Langlois MR. Circulating Oxidized Low-Density Lipoprotein: A Biomarker of Atherosclerosis and Cardiovascular Risk? Clinical Chemistry and Laboratory Medicine. 2009; 47(2): 128-137.
Holvoet P, Jenny NS, Schreiner PJ, Tracy RP, and Jacobs DR. The Relationship Between Oxidized LDL and Other Cardiovascular Risk Factors and Subclinical CVD in Different Ethnic Groups: The Multi-Ethnic Study Of Atherosclerosis (MESA). Atherosclerosis. 2007; 194(1): 245-252.
Gomez M, Vila J, Elosua R, et al. Relationship of Lipid Oxidation with Subclinical Atherosclerosis and 10-Year Coronary Events in General Population. Atherosclerosis. 2014; 232(1): 134-140.
Kato R, Mori C, Kitazato K, et al. Transient Increase in Plasma Oxidized LDL During the Progression of Atherosclerosis in Apolipoprotein E Knockout Mice. Arteriosclerosis, Thrombosis, and Vascular Biology. 2009; 29(1): 33-39.
Itabe H. Oxidative Modification of LDL: Its Pathological Role in Atherosclerosis. Clinical Reviews in Allergy & Immunology. 2009; 37(1): 4-11.
Uno K and Nicholls SJ. Biomarkers of Inflammation and Oxidative Stress in Atherosclerosis. Biomarkers in Medicine. 2010; 4(3): 361-373.
Wu M, Bian Q, Liu Y, et al. Sustained Oxidative Stress Inhibits NF-κB Activation Partially viaInactivating the Proteasome. Free Radical Biology & Medicine. 2009; 46(1): 62–69.
Bui QT, Prempeh M, and Wilensky RL. Atherosclerotic Plaque Development. The International Journal of Biochemistry & Cell Biology. 2009; 41(11): 2109–2113.
Maiolino G, Rossitto G, Caielli P, Bisogni V, Rossi GP, and Calo LA. The Role of Oxidized Low-Density Lipoproteins in Atherosclerosis: The Myths and the Facts. Mediators of Inflammation. 2013; 2013: 1-13.
Back M, Ketelhuth DFJ, and Agewall S. Matrix Metalloproteinases in Atherothrombosis. Progress in Cardiovascular Diseases. 2010; 52(5): 410-428.
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