Pengaruh Amitriptyline Dosis Lethal pada Bangkai Tikus Rattus Norvegicus strain Wistar terhadap Pertumbuhan Larva Musca Sp.

Authors

  • Puji Rahman Program Studi Pendidikan Dokter Universitas Brawijaya
  • Aswin Djoko B Laboratorium Parasitologi Fakultas Kedokteran Universitas Brawijaya
  • Wening Prastowo Laboratorium Kedokteran Forensik Rumah Sakit Umum Dr. Saiful Anwar Malang

DOI:

https://doi.org/10.21776/ub.jkb.2010.026.02.6

Abstract

ABSTRAK
Kata Kunci:  Amitriptyline, Musca sp., pertumbuhan larva
Peran Post Mortem Interval (PMI) sangat penting untuk memperkirakan waktu kematian yang dapat diketahui dengan
menentukan  umur  larva  lalat  yang  terdapat  pada  jenazah.  Pertumbuhan  larva  lalat  sangat  dipengaruhi  oleh  suhu
lingkungan,  makanan,  kelembapan,  intensitas  cahaya,  dan  kontaminan.  Keberadaan  obat  antidepresan  dapat
mengacaukan estimasi waktu kematian hingga 77 jam. Penelitian ini merupakan penelitian eksperimental menggunakan
media  dua  bangkai  tikus.  Tikus  pertama  dibunuh  dengan  cara  diberikan  amitriptyline  100  mg  peroral  (LD50  =  350
mg/kgBB).   Tikus kedua dibunuh dengan cara dislokasi cervical. Kedua bangkai ini dimasukkan ke 2 kandang yang telah
berisi masing-masing 50 lalat Musca sp. Dilakukan pengamatan setiap 12 jam mulai dari larva lalat stadium satu sampai
lalat dewasa. Setiap media tumbuh diambil 10 larva secara acak setiap hari untuk diukur panjang dan berat serta durasi
pertumbuhannya. Hasil penelitian menunjukkan bahwa pertumbuhan larva pada media dengan kandungan amitriptyline
berbeda secara signifikan dibandingkan pertumbuhan larva pada media tanpa kandungan amitriptyline. Panjang larva
pada media tumbuh dengan kandungan amitriptyline lebih panjang, berat larva pada media tumbuh dengan kandungan
amitriptyline lebih berat dan durasi pencapaian stadium lebih lambat sejak hari ke-3a (p < 0,005). Hasil penelitian ini
menunjukkan bahwa amitriptyline dapat memperlambat pertumbuhan larva lalat Musca sp.

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References

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Published

2013-03-10

Issue

Vol. 26 No. 2 (2010)

Section

Research Article

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