Profilin Menginduksi Ekspresi TLR-11, Il-6, dan TNF- sebagai Kandidat Prediktor Disfungsi Adiposit akibat Infeksi Toxoplasma Gondii α

Authors

  • Sudjari Sudjari Laboratorium Parasitologi Fakultas Kedokteran Universitas Brawijaya Malang
  • M. Rasjad Indra Laboratorium Fisiologi Fakultas Kedokteran Universitas Brawijaya Malang
  • Hendra Susanto Laboratorium Anatomi dan Fisiologi Manusia Universitas Negeri Malang
  • Lulik Inggarwati Laboratorium Ilmu Bedah Rumah Sakit Saiful Anwar Malang

DOI:

https://doi.org/10.21776/ub.jkb.2010.026.02.5

Abstract

ABSTRAK
Sindrom metabolik adalah sekelompok faktor risiko yang terdiri dari hipertensi, dislipidemia, gangguan intoleransi glukosa
dan obesitas dan memiliki hubungan dengan pathomechanism penyakit jantung. Peran jaringan adiposa pada penyakit
menular dan perubahan kualitas adipocyte setelah terpapar patogen belum terungkap. Di sisi lain, prevalensi penyakit
infeksi di negara berkembang masih tinggi. Beberapa studi melaporkan bahwa ada hubungan antara infeksi T gondii dan
fungsi  jaringan  adiposa.  Profilin  adalah  komponen  membran  T  gondii  yang  dapat  merangsang  ekspresi  sitokin
proinflammation IL-12 dan lain sel dendrit melalui pengikatan dengan TLR-11. Ini dapat terjadi pada sel-sel lemak, memicu
disfungsi adipocyte yang mengarah ke sindrom metabolik. Penelitian sebelumnya telah menyebutkan bahwa sindrom
metabolik  yang  berhubungan  dengan  jaringan  lemak  selalu  disebut  lemak  visceral,  sedangkan  lemak  subkutan  juga
berpotensi menjadi patogen. Penelitian ini adalah untuk membuktikan pengaruh paparan profilin untuk ekspresi TLR-11,
IL-6, dan TNF-dalam kultur jaringan adiposa subkutan yang diukur dengan teknik ELISA. Hasil penelitian menunjukkan
bahwa efek paparan profilin, dosis 5 15 dan 25 ng / ml peningkatan IL-6, dan TNF-tingkat, tetapi menurun tingkat TLR-11 (p
= 0,000). Penelitian ini membuktikan paparan profiling menyebabkan disfungsi adipocyte melalui peningkatan tingkat
adipocytokine proinflamasi sebagai patomekanisme sindrom metabolik yang berhubungan dengan jaringan adiposa.
Kata Kunci : A  profilin Toxoplasma gondii, TLR-11
dipocytokines (IL-6, TNF α), adipose subkutan, disfungsi adiposit,

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References

DAFTAR PUSTAKA

Tuncman G,Erbay E, Hom X, et al. A Genetic Variant at the Fatty Acid-Binding Protein aP2 Locus Reduces the Risk for Hypertriglyceridemia, Type 2 Diabetes, and Cardiovascular Disease. Proceedings of the National Academy of Sciences of The United States of America. March 16,2006; hal. 6970-6975.

Rajala WM and Scherer EP. Minireview: The Adipocyte-at the Crossroads of Energy. Homeostasis, Inflammation, and Atherosclerosis. Endocrinology. 2003; 144(9):3765–3773.

Desruisseaux SM, Nagajyothi, Maria ET, Herbert BT, and Phillip ES. Adipocyte, Adipose Tissue, and Infectious Disease. Infection and Immunity. 2006; 75(3):1066-1078.

Olgica D and Vladimir M. Murine Model of Drug-induced Reactivation of Toxoplasma gondii. Acta Protozoologica.2001;40:99-106.

Charron AJ and Sibley LD. Host Cells: Mobilizable Lipid

Resources for TheI Intracellular Parasite Toxoplasma Gondii. Journal of Cell Science. 2002;115:3049-3059.

Frederic P, Denis, Denis and Yves. Responses of Adipose and Muscle Lipoprotein Lipase to Chronic Infection and Subsequent Acute Lipopolysaccharide Challenge. Clinical and Diagnostic Laboratory Immunology. 2002;9(4):771–776.

Yarovinsky F, Zhang D, Andersen JF, et al. TLR-11 Activation of Dendritic Cells by a Protozoan Profilin-Like Protein. Science Magazine. 2005; 308 (5728):1626-1629.

Lauw FN, Caffrey DR, and Golenbock DT. Of Mice and Man: TLR11 (Finally) Finds Profilin. Trends in Immunology. 2005;26(10):509-511.

Cheng-Hu L, Fabiana SM, Rishu G, et al. Diacylglycerol Kinase ζ Regulates Microbial Recognition and Host Resistance to Toxoplasma gondii. The Journal of Experimental Medicine. 2007; 204(4):781–792.

Sambrook J and Russell DW. Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press;2001.

Fruhbeck G, Ambrozi GJ, Muruzabal JF, and Burrel MA. The Adipocyte: A Model for Integration of Endocrine and Metabolic Signaling in Energy Metabolism Regulation. American Journal of Physiology Endocrinology and Metabolism. 2001; 280(6):827-9.847.

Bays H, Blonde L, and Rosenson R. Adiposopathy: How do Diet, Exercise and Weight Loss Drug Therapies Improve Metabolic Disease in Overweight Patients? Expert Review of Cardiovascular Therapy. 2006;4(6:95-871.

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Published

2013-03-10

Issue

Vol. 26 No. 2 (2010)

Section

Original Article

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