Peran Ekspresi Interleukin (IL)-4 dalam Apoptosis Epitel Bronkiolus Mencit Asma

Authors

  • Runiawan Condro S Laboratorium Ilmu Kesehatan Anak Rumah Sakit Umum Dr. Saiful Anwar Malang

DOI:

https://doi.org/10.21776/ub.jkb.2010.026.02.4

Abstract

ABSTRAK
Inflamasi kronis pada asma merupakan salah satu faktor yang mempengaruhi keparahan, eksaserbasi, dan remodeling
jalan nafas yang ditandai peningkatan apoptosis epitel bronkiolus. Interleukin (IL)-4 merupakan salah satu sitokin tipe Th2
yang merupakan petanda proses inflamasi. Tujuan penelitian ini adalah untuk membuktikan hubungan antara ekspresi IL-
4  dan  apoptosis  epitel  pada  bronkiolus  mencit  asma.  Penelitian  dilakukan  dengan  desain  randomized  control  group
menggunakan 18 mencit galur Balb/c yang dibagi dalam kelompok asma dan kontrol. Kelompok asma disensitisasi dengan
ovalbumin secara intraperitoneal pada hari ke-0 dan 14, diikuti dengan cara inhalasi setiap 2-3 hari selama 6 minggu.
Ekspresi IL-4 pada bronkiolus diperiksa dengan metode imunohistokimia, sedangkan apoptosis epitel bronkiolus dengan
metode  TUNEL.  Analisis  statistik  menggunakan  independent  sample  t-test,  Mann-Whitney  U  test,  dan  regresi  linier
dengan  interval  kepercayaan  95%.  Jumlah  apoptosis  epitel  bronkiolus  dan  ekspresi  IL-4  meningkat  bermakna  pada 2kelompok asma (p=0,000).Terdapat korelasi positif kuat (r=0,78; r =0,56, p=0,012, y=4,28+0,58x) antara IL-4 dan apoptosis
epitel pada bronkiolus. Peningkatan ekspresi IL-4 mempengaruhi peningkatan apoptosis epitel bronkiolus, namun bukan
merupakan satu-satunya variabel yang mempengaruhi peningkatan apoptosis epitel bronkiolus.
Kata Kunci : Asma, apoptosis epitel, bronkiolus, IL-4,   ovalbumin
mencit

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Published

2013-03-10

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Research Article