Peran Ekspresi Interleukin (IL)-4 dalam Apoptosis Epitel Bronkiolus Mencit Asma
DOI:
https://doi.org/10.21776/ub.jkb.2010.026.02.4Abstract
ABSTRAKInflamasi kronis pada asma merupakan salah satu faktor yang mempengaruhi keparahan, eksaserbasi, dan remodeling
jalan nafas yang ditandai peningkatan apoptosis epitel bronkiolus. Interleukin (IL)-4 merupakan salah satu sitokin tipe Th2
yang merupakan petanda proses inflamasi. Tujuan penelitian ini adalah untuk membuktikan hubungan antara ekspresi IL-
4 dan apoptosis epitel pada bronkiolus mencit asma. Penelitian dilakukan dengan desain randomized control group
menggunakan 18 mencit galur Balb/c yang dibagi dalam kelompok asma dan kontrol. Kelompok asma disensitisasi dengan
ovalbumin secara intraperitoneal pada hari ke-0 dan 14, diikuti dengan cara inhalasi setiap 2-3 hari selama 6 minggu.
Ekspresi IL-4 pada bronkiolus diperiksa dengan metode imunohistokimia, sedangkan apoptosis epitel bronkiolus dengan
metode TUNEL. Analisis statistik menggunakan independent sample t-test, Mann-Whitney U test, dan regresi linier
dengan interval kepercayaan 95%. Jumlah apoptosis epitel bronkiolus dan ekspresi IL-4 meningkat bermakna pada 2kelompok asma (p=0,000).Terdapat korelasi positif kuat (r=0,78; r =0,56, p=0,012, y=4,28+0,58x) antara IL-4 dan apoptosis
epitel pada bronkiolus. Peningkatan ekspresi IL-4 mempengaruhi peningkatan apoptosis epitel bronkiolus, namun bukan
merupakan satu-satunya variabel yang mempengaruhi peningkatan apoptosis epitel bronkiolus.
Kata Kunci : Asma, apoptosis epitel, bronkiolus, IL-4,  ovalbumin
mencit
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