Efek Ekstrak Etanol Daun Salam terhadap Ekspresi VEGF Podosit Glomerulus Tikus Diabetes Melitus
DOI:
https://doi.org/10.21776/ub.jkb.2015.028.04.1Abstract
Stres oksidatif pada diabetes melitus meningkatkan ekspresi VEGF podosit glomerulus sehingga menyebabkan disfungsi endotel. Daun salam mengandung antioksidan yang dapat menghambat ekspresi VEGF. Penelitian ini membuktikan  pemberian Ektrak Etanol Daun Salam (EEDS) dapat menurunkan ekspresi VEGF podosit glomerulus tikus Sprague dawley diabetes melitus. Sampel 20  tikus Sprague dawley jantan diabetes melitus dibagi 4 kelompok: Kelompok EEDS (-), kelompok EEDS(+): dosis 150mg/200grBB; 300mg/200grBB; 450mg/200grBB selama 15 hari. Rerata allred score ekpresi VEGF pada kelompok kontrol (1,2±1,09), dosis satu (1,3±1,15), dosis dua (0,50±1,00), dosis tiga (0,00). Analisa Krusskall Wallis menunjukkan tidak ada perbedaan ekspresi VEGF podosit glomerulus diantara masing-masing kelompok (p=0,20). Dosis 450mg/200gr BB dapat menurunkan ekspresi VEGF  walaupun tidak bermakna (p=0,07). Berdasarkan penelitian ini disimpulkan, EEDS  tidak dapat  menurunkan ekspresi VEGF  podosit glomerulus tikus Sprague dawley diabetes melitus.Kata Kunci: Daun salam, diabetes melitus, VEGF podosit glomerulus
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Kota SK, Meher LK, Jammula S, Kota SK, Krishna SVS, and Modi KD. Aberrant Angiogenesis: The Gateway to Diabetic Complications. Indian Journal of Endocrinology and Metabolism. 2012; 16(6): 918–930.
Mironidou-Tzouveleki M, Tsartsalis S, and Tomos C. Vascular Enothelial Growth Factor (VEGF) in the Pathogenenesis of Diabetic Nephropathy of Type I Diabetes Mellitus. Current Drug Target. 2011; 12(1): 107-114.
Khamaisi M, Schrijvers BF, De Vriese AS, Raz I, and Flyvbjerg A. The Emerging Role of VEGF in Diabetic Kidney Disease. Nephrology Dialysis Transplantation. 2003 Aug; 18(8): 1427-1430.
Kusuma IW, Kuspradini H, Arung ET, et al. Biological Activity and Phytochemical Analysis of Three Indonesian Medicinal Plants, Murraya koenigii, Syzygium polyanthum and Zingiber purpurea. Journal of Acupuncture and Meridian Studies. 2011; 4(1): 75-79.
Lelono RAA and Tachibana S. Preliminary Studies of Indonesian Eugenia polyantha Leaf Extracts as Inhibitors of Key Enzymes for Type 2 Diabetes. Journal of Medical Sciences. 2013; 13(2): 103-110.
Giacco F and Brownlee M. Oxidative Stress and Diabetic Complication. Circulation Research Archives. 2010;107(9): 1058-1070.
Nangaku M. Chronic Hypoxia and Tubulointerstitial Injury: A Final Common Pathway to End-Stage Renal Failure. Journal of American Society of Nephrology. 2006; 17(1): 17-25.
Cha DR, Kang YS, Han SY, et al. Vascular Endothelial Growth Factor is Increased During Early Stage of Diabetic Nephropathy in Type II Diabetic Rats. Journal of Endocrinologi. 2004; 183(1): 183-194
Tufro A and Veron D. VEGF And Podocytes in Diabetic Nephropathy. Seminars in Nephrology. 2012; 32(4): 385-393
Har LW and Ismail IS. Antioxidant Activity, Total Phenolic and Total Flavonoids of Syzygium Polianthum (Wight) Walp Leaves. International Journal of Medicinal and Aromatic Plants. 2012; 2(2): 219-228.
Departemen Kesehatan Republik Indonesia. Materia Medika Indonesia. Jakarta: Depkes RI; 1980.
Yohana A dan Yovita A. Khasiat Tanaman Obat. Jakarta: Pustaka Buku Murah; 2008
Mun'im A dan Hanani E. Fitoterapi Dasar Jakarta: Dian Rakyat; 2011
Sumono A dan Wulan A. The Use of Bay Leaf (Eugenia polyantha Wight) in Dentistry. Dental Journal. 2008; 41(3): 147-150.
Cao Z and Cooper ME. Pathogenesis of Diabetic Nephropathy. Journal of Diabetes Investigation. 2011; 2(4): 243-247.
Sastroasmoro S dan Ismael S. Dasar-dasar Metodologi Penelitian Klinis. 3rd ed. Jakarta: Sagung Seto; 2008.
Salmon AH, Neal CR, Bates DO, and Harper SJ. Vascular Endotheial Growth Factor Increaes the Ultrafiltration Coefficient in Isolated Intact Wistar Rat Glomeruli. The Journal of Physiology. 2006; 570(1): 141-156.
Szkudelski T. The Mechanisme of Alloxan and Streptozotocin Action in B Cell of the Rat Pancreas. Physiological Research. 2001; 50(6): 537-546.
Akbarzadeh A, Norouzian D, Mehrabi MR, et al. Induction of Diabetes by Streptozotocin in Rats. Indian Journal of Clinical Biochemistry. 2007; 22(2): 60-64.
Arora S, Ojha SK, and Vohora D. Characterisation of Streptozotocin Induced Diabetes Mellitus in Swiss Albino Mice. Global Journal of Pharmacology. 2009; 3(2): 81-84.
Sulistyoningrum E dan Setiawati. Phaleria Macrocarpha Reduces Glomerular Growth Factor Expression in Alloxan- Induced Diabetic Rats. Universa Medicina. 2013; 32(2): 71-79.
Tesch GH and Allen TJ. Rodent Models of Streptozotocin-Induced Diabetic Nephropathy. Nephrology. 2007; 12(3): 261-266.
Studiawan H, Santosa MH. Uji Aktifitas Penurunan Kadar Gula Darah Ekstrak Daun Eugenia Poliantha pada Mencit yang Diinduksi Alloxan. Media Kedokteran Hewan. 2005; 21(2): 62-65.
Liu E, Morimoto M, Kitajima S, et al. Increased Expression of Vascular Endothelial Growth Factor in Kidney Leads to Progressive Impaiment of Glomerular Functions. Journal American Society of Nephrology. 2007; 18(7): 2094-2104.
Schlingemann RO, Van Noorden CJ, Diekman MJ, et al. VEGF Levels in Plasma in Relation to Platelet Activation, Glycemic Control,and Microvascular Complications in Type 1 Diabetes. Diabetes Care. 2013; 36(6): 1629–1634.
Schrijvers BF, Flyvbjerg A, and De Vriese AS. The Role of Vascular Endothelial Growth Factor (VEGF) in Renal Pathophysiology. Kidney International. 2004; 65(6): 2003-2017.
Murnah. Pengaruh Ekstrak Etanol Mengkudu (Morinda citrifolia L) terhadap Diabetik Nefropati pada Tikus Srague Dawley yang Diinduksi Streptozotosin dengan Kajian VEGF dan Mikroalbuminuria. [Tesis]. Universitas Diponegoro, Semarang. 2011.
Christoper C. Osmoregulation II. Animal Sciences Bio 131 with Thometz at Univercity of California-Santa cruz. (Online) 2012. https://www.studyblue.com/ notes/note/n/osmoregulation- ii/deck/6365072.
Sastrawan IGP dan Suwitra K. Peran Hipoksia pada Patogenesis Penyakit Ginjal. Jurnal Penyakit Dalam. 2008; 9(1): 75-84.
Melhelm MF, Craven PA, Liachenko J, and DeRubertis FR. α-Lipoic Acid Attenuates Hyperglycemia an Prevents Glomerular Mesangial Matrix Expansion in Diabetes. Journal of American Society of Nephrology. 2002; 13(1): 108-116.
Nakagawa T, Kosugi T, Haneda M, Rivard CJ, and Long DA. Abnormal Angiogenesis in Diabetic Nephropathy. Diabetes. 2009; 58(7): 1471-1478.
Sivaskandarajah GA, Jeansson M, Maezawa Y, Eremina V, Baelde HJ, and Quaggin SE. VEGF-A Protects the Glomerular Microvasculature in Diabetes. Diabetes Journal. 2012; 61(11): 2958-2966.
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