Probiotik Tidak Mempengaruhi Profil Sel Imun Adaptif pada Infeksi Escherichia coli
DOI:
https://doi.org/10.21776/ub.jkb.2013.027.04.1Abstract
Probiotik diketahui memiliki peran potensial dalam manajemen beberapa kondisi patologis. Salah satu mekanisme penting dari sistem imun adalah konsep keseimbangan sitokin yang diperankan oleh sel Th1, Th2, Tregulator, dan sel Th17. Penelitian ini dilakukan untuk mengetahui pengaruh pemberian Lactobacillus rhamnosus dan L. acidophilus terhadap ekspresi sitokin Th1, Th2, Tregulator, Th17 pada sistem imun adaptif sistemik mencit yang terpapar oleh Lipopolisakarida (LPS) Escherichia coli. Tiga puluh dua ekor mencit BALB/c jantan dibagi menjadi 4 kelompok yaitu kelompok kontrol, LPS (mendapat LPS per sonde pada hari pertama), probiotik (mendapat probiotik per sonde selama 7 hari), LPS-Probiotik (mendapat LPS per sonde pada hari pertama, dilanjutkan probiotik per sonde selama 7 hari berikutnya). Pada hari ke-9 semua mencit dibunuh dan diambil sampel darahnya. Sel penghasil sitokin Th1 (IL-2, IFN-γ), Th2 (IL-4, IL-5), Tregulator (IL-10, TGF-β) dan Th17 (IL-17, IL-23) diperiksa dengan metode flowcytometri. Kelompok Probiotik, LPS dan LPS-Probiotik menunjukkan penurunan semua sel penghasil sitokin IL-2, IL-17, IL-23, IL-4, IL-10 dan TGF-β yang berbeda bermakna dibanding kontrol, sedangkan kelompok LPS-Probiotik tidak menunjukkan perbedaan yang bermakna pada sel penghasil sitokin bila dibandingkan kelompok LPS. Pemberian probiotik pada penelitian ini tidak berpengaruh terhadap ekspresi sitokin sel Th1, Th2, Tregulator dan Th17 pada darah mencit yang terpapar LPS.Kata Kunci: LPS, probiotik, respon imun adaptif sistemik
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Black RE, Cousens S, Johnson HL, et al. Child Health Epidemiology Reference Group of WHO and UNICEF: Global, Regional, and National Caused of Child Mortality in 2008: A Systematic Analysis. The Lancet. 2010; 375(9730): 1969-1987.
Subagyo B dan Santoso N. Diare Akut. Di dalam: Buku Ajar Gastroentero-Hepatologi IDAI. Jakarta: Badan Penerbit IDAI; 2010; hal. 87-118.
James T. Health-benefit Claims for Probiotic Products. Clinical Infectious Diseases. 2008; 46: S122-124.
Hajela N, Nair GB, and Ganguly NK. Are Probiotics a Feasible Intervention for Prevention of Diarrhoea in the Developing World? Gut Pathogen. 2010; 2: 2-5.
Wardlaw T, Salama P, Brocklehurst C, et al. Diarrhoea: Why Children are Still Dying and What Can be Done. The Lancet. 2010; 375: 870-872.
Saavedra J and Tschernia A. Human Studies with Probiotics and Prebiotics: Clinical Implications. British Journal of Nutrition. 2002; 87: 241-246.
Canani R, Cirillo P, Terrin G, et al. Probiotics for Treatment of Acute Diarrhea in Children: Randomized Clinical Trial of Five Different Preparations. British Medical Journal. 2007; 335: 340-345.
Freedman A and Lewis S. Review Article: The Use of Biotherapeutic Agents in the Preventsion and Treatment of Gastrointestinal Disease. Alimentary Pharmacology and Therapeutics. 1998; 12: 807-822
Subijanto, Suparto P, Suhartono T, dan Judajana. Gangguan Sistem Imun Mukosa Intestinal. Surabaya: Divisi Gastroenterologi Lab/SMF Ilmu Kesehatan Anak RS dr Soetomo-FK UNAIR; 2003.
Judarwanto W. Imunologi Mukosa Saluran Cerna. (Online) 2008. http://alergianak.bogspot.com [diakses 10 Februari 2010].
Magrone T and Jirillo E. The Cytokine-mediate Network in Health and Disease with Special Reference to the Gut Immune System Regulation. Journal of Clinical Ligand Assay. 2009; 14: 350-359.
Alexander C and Rietschel E. Bacterial Lipopolysaccharides and Innate Immunity. Journal of Endotoxin Research. 2001; 7(3): 167-202.
Iskandar D. Pengaruh Pemberian Probiotik terhadap Keseimbangan Sel Th1, Th2 dan Treg pada Respon Imun Mukosa Usus. [Tesis]. Universitas Airlangga, Surabaya. 2009.
Mohr AJ, Leisewitz AL, Jacobson LS, et al. Effect of Early Enteral Nutrition on Intestinal Permeability, Intestinal Protein Loss, and Outcome in Dogs with Severe Parvoviral Enteritis. Journal of Veterinary Internal Medicine. 2003; 17: 791-798.
Pratiwi TA. Pengaruh Pemberian L.rhamnosus dan L.acidophilus terhadap Sekresi sitokin Th1, Th2, Treg, Th17 pada Mukosa Usus Mencit yang Terpapar Lipopolisakarida E.coli. [Tesis]. Universitas Brawijaya, Malang. 2010.
Sansonetti P and Di Santo J. Debugging How Bacteria Manipulate the Imune Response. Journal of Immunology. 2007:149-61
Pulendran B, Kumar P, Cutler C, Mohamadzadeh M, Dyke T, and Banchereau J. Lipopolysaccahrides from Distinct Pathogens Induce Different Classes of Immune Responses In Vivo. The Journal of Immunology. 2001; 167: 5067-5076.
Mukherjee S, Chen L, Papadimos T, Huang S, Zuraw B, and Pan Z. LPS driven Th2 Cytokine Production in Macrophages is Regulated by Both MyD88 and TRAM. Journal of Biological Chemistry. 2009; 284(43): 29391-29398.
Lauw F, Hove T, Dekkers P, Jonge E, Deventser S, and Poll T. Reduced Th1, but not Th2, Cytokine Production by Lymphocytes after In Vivo Exposure of Healthy Subjects to Endotoxin. Infection and Immunity. 2000; 68: 1014-1018.
Larsson R, Rocksen D, Lilliehook B, Jonsson A, and Bucht A. Dose-dependent Activation of Lymphocytes in Endotoxin-induced Airway Inflammation. Infection and Immunity. 2000; 68: 6962-6969.
Couper K, Blount D, and Riley E. IL-10: The Master Regulator of Immunity to Infection. The Journal of Immunology. 2008; 180: 5771-5777.
Eisenbarth S, Piggot D, Huleatt J, Visintin I, Herrick C, and Bottomly K. Lipopolysaccharide-enhanced, Toll-like Receptor 4-dependent T Helper Cell Type 2 Responses to Inhaled Antigen. Journal of Experimental Medicine. 2002; 196: 1645-1651.
Min J, Park S, Rhim T, Park S, Jang A, and Uh S. LPS Induced Inhibition of IL-5 and Eotaxin-2 Production in Atopic Asthmatics. Clinical and Experimental Immunology. 2007; 147: 440-448.
Cross M, Ganner A, Teilab D, and MF L. Patterns of Cytokine Induction by Gram-positive and Gram-negative Probiotic Bacteria. Federation of European Microbiological Societies Immunology and Medical Microbiology. 2004; 42: 173-180.
Perdigon G, Galdeano C, Valdez J, and Medici M. Interaction of Lactic Acid Bacteria with the Gut Immune System. European Journal of Clinical Nutrition. 2002; 56: 21-26.
Szajewska H, Ruszczynski M, and Radzikowski A. Probiotics in the Prevention of Antibiotic-Associated Diarrhea in Children: A Meta-Analysis of Randomized Controlled Trials. Journal of Pediatric Gastroenterology and Nutrition. 2006; 149: 367-372.
Pochard P, Gosset P, Grangette C, et al. Lactic Acid Bacteria Inhibit TH2 Cytokine Production by Mononuclear Cells from Allergic Patients. Journal of Allergy and Clinical Immunology. 2002; 110(4): 617-623.
Cross ML. Microbes versus Microbes: Immune Signals Generated by Probiotic Lactobacilli and Their Role in Protection against Microbial Pathogens. Federation of European Microbiological Societies Immunology and Medical Microbiology. 2002; 34: 245-253.
Kekkonen R, Lummela N, Karjalainen H, Latvala S, Tynkkynen S, and Jarvenpaa S. Probiotics Intervention Has Strain Spesific Anti-inflammatory Effects in Healthy Adults. World Journal of Gastroenterology. 2008; 14: 2029-2036.
Delcenserie V, Martel D, Lamoureux M, Amiot J, Boutin Y, and Roy D. Immunomodulatory Effects of Probiotics in the Intestinal Tract. Current Issues in Molecular Biology. 2008; 10: 37-54.
Braat H, Brande J, Tol E, Hommes D, Peppelenbosch M, and Deventer S. Lactobacillus rhamnosus Induces Peripheral Hyporesponsiveness in Stimulated CD4+ T Cells via Modulation of Dendritic Cell Function. American Journal of Clinical Nutrition. 2004; 80: 1618-1625.
Lavasani S, Dzhambazov B, Nouri M, Fak F, Buske S, and Molin G. A Novel Probiotic Mixture Exerts a Therapeutic Effect on Experimental Autoimmune Encephalomyelitis Mediated by IL-10 Producing Regulatory T Cells. PloS ONE. 2010; 5(2): 1-11.
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