Probiotik Menurunkan Ekspresi TLR2 dan Aktivasi NF-Kb p50 pada Sel Mononuklear Darah Mencit yang T erpajan Lipopolisakarida E.Coli

Authors

  • Ni Luh Putu HM Laboratorium Ilmu Kesehatan Anak Rumah Sakit Umum Dr . Saiful Anwar Malang
  • Sumakto Sumakto Laboratorium Ilmu Kesehatan Anak Rumah Sakit Umum Dr . Saiful Anwar Malang
  • Sanarto Santoso Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Brawijaya Malang

DOI:

https://doi.org/10.21776/ub.jkb.2011.026.03.11

Abstract

Beberapa tahun terakhir banyak penelitian dilakukan untuk melihat penggunaan probiotik untuk pencegahan maupun untuk  pengobatan.  Salah  satu  penggunaan  probiotik  adalah  dalam  pengobatan  diare.  Penggunaan  probiotik  tersebut berdasarkan  respon  imun  yang  dapat  ditimbulkan  oleh  probiotik  baik  di  dalam  mukosa  usus  maupun  di  sel-sel mononukklear .  Penelitian ini  bertujuan untuk mengetahui pengaruh probiotik terhadap aktivasi T oll-like receptor (TLR2), TLR4, Nuclear Factor Kappa B p50 dan p65 pada sel mononuklear darah yang terpajan dengan LPS E.coli.Mencit Balb/c dibagi  dalam  4  kelompok  perlakuan.  Satu  kelompok  mendapat  LPS  pada  hari  pertama  dilanjutkan  dengan  probiotik selama  7 hari, satu  kelompok LPS saja,  satu  kelompok probiotik selama  7 hari dan kelompok kontrol tanpa perlakuan. Dilakukan pemeriksaan  imunositokimia pada sel  mononuklear yang  mengekspresikan TLR2, TLR4, dan aktivasi  p50 NF-kB dan  p65  NF-kB. Hasil  penelitian  menunjukkankelompok  LPS  dan  probiotik  peningkatan  ekspresi  TLR2  dan  TLR4,  serta aktivasi NF-kB p50 dan p65, sedangkan pemberian pemberian probiotik mampu menurunkan ekspresi TLR2 dan aktivasi NF-kB p50 (p<0,05) yang disebabkan oleh LPS. Probiotik pada keadaan pajanan dengan LPS E.coli lebih mempengaruhi penurunan  ekspresi  TLR2  dan  menurunkan  aktivasi  NFkB  p50,  yang  diharapkan  mampu menurunkan  reaksi  inflamasi karena  pemberian  LPS.

Downloads

Download data is not yet available.

References

Suraatmaja S. Diare. Di dalam: Suraatmaja S (Ed). Kapita Selekta Gastroenterologi Anak. Jakarta: Sagung Seto; 2007.

Kresno SB. Imunologi: Diagnosis dan Prosedur Laboratorium. Edisi 5. Jakarta: Badan Penerbit Fakultas Kedokteran Universitas Indonesia; 2010.

Alexander C and Rietschel ET. Bacterial Lipopolysaccharides and Innate Immunity. Journal of Endotoxin Research. 2001; 7(3): 167-204.

Covert MW, Leung TH, Gaston JE, and Baltimore D. Achieving stability of Lipopolysaccharide-Induced NF-kB activation. Science. 2005; 309(5742): 1854-1857.

Suparto P. Imunologi Intestinal. Di dalam: Suparto P (Ed). Gangguan Sistem Imun Mukosa Intestinal. Surabaya: Graha Masyarakat Ilmiah Kedokteran Fakultas Kedokteran Universitas Airlangga; 2003.

Ljungh A and Wadstrom T. Lactic Acid Bacteria as Probiotic. Current Issues Intestinal Microbiol. 2006; 7(2): 73-90.

Isolauri E, Sutas Y, Kankaanpaa P, Arvilommi H, and Salminen S. Probiotics: Effects on Immunity. The American Journal Clinical Nutrition. 2001; 73(2): 444S-550S.

FAO/WHO. Health and Nutritional Properties of Probiotics in Food including Powder Milk with Live Lactic Acid Bacteria. Report of a joint FAO/WHO Expert Consultion on Evaluation of Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria. Cordoba, Argentina 2001; p. 1-32.

Floch MH, Walker WA, Guandalini S, et al. Recommendation for Probiotics Use-2008. Journal of Clinical Gastroenterology. 2008; 42: 104-108.

del Giudice MM and Brunese FP. Probiotics, Prebiotics, and Allergy in Children What's New in the Last Year? Journal of Clinical Gastroenterology. 2008; 42: 205-208.

Netea MG, Graaf C, Meer JW, and Kulberg BJ. Toll-Like Receptors and the Host Defense Against Microbial Pathogens. Journal of Leukocyte Biology. 2004; 75(5): 749-755.

Lee J-H, Lee B, Lee H-S, et al. Lactobacillus Suntoryeus Inhibits Pro-Inflammatory Cytokine Expression And TLR-4 Linked NF-Kb Activation In Experimental Colitis. International Journal of Colorectal Disease. 2009; 24(2): 231-237.

Lebeer S, Vanderleyden J, and Keersmaecker SCJD. Genes and Molecules of Lactobacilli Suporting Probiotic Action. Microbiology and Molecular Biology Review. 2008; 72(4): 728-764.

Lin PW, Myers LE, Ray L, et al. Lactobacillus Rhamnosus Block Inflammatory Signaling In Vivo Via Reactive Oxygen Species Generation. Free Radical Biology & Medicine. 2009; 47(8):1205-1211.

Ishida-Fujii K, Sato R, Goto S, et al. Prevention of Pathogenic Escherichia coli Infection in Mice and stimulation of Macrophage Activation in Rats by an Oral Administration of Probiotic Lactobacillus casei I-5. Bioscience Biotechnology Biochemistry. 2007; 7(4): 866-873.

Cosseau C, Devine DA, Dullaghan E, et al. The Commensal Streptococcus salivarius K12 Downregulates the Innate Immune Responses of Human Epithelial Cells and Promotes Host-Microbe Homeostasis. Infection and Immunity. 2008; 76(9): 4163-4175.

Bai A, Ouyang Q, Xiao X, and Li SF. Probiotics Modulate Inflammatory Cytokine Secretion from Inflamed Mucosa in Ulcerative Colitis. International Journal of Clinical Practice. 2006; 60(3): 284-288.

Watanabe T, Nishio H, Tanigawa T, et al. Probiotic Lactobacillus Casei Strain Shirota Prevents Indomet hacin-Induced Small Intestinal Injury: Involvement of Lactic Acid. American Journal of Phsiology-Gastrointestinal and Liver Physiology. 2009; 297(3): 506-513.

Galdeano CM and Perdigon G. The Probiotic Bacterium Lactobacillus Casei Induces Activation of the Gut Mucosal Immune System Through Innate Immunity. Clinical and Vaccine Immunology. 2006; 13(2): 219-226.

Galdeano CM, LeBlanc AdMd, Vinderola G, Bonet MEB, and Perdigon G. Proposed Model: Mechanism of Immunomodulation Induced by Probiotic Bacteria. Clinical and Vaccine Immunology. 2007; 14(5): 485-492.

Hajela N, Nair GB, and Ganguly NK. Are Probiotics a Feasible Intervention for Prevention of Diarrhoea in the Developing World? Gut Pathogens. 2010; 2: 10-15.

Purswani MU, Eckert SJ, Arora HK, and Noel GJ. Effect of Ciprofloxacin on Lethal and Sublethal Challenge with Endotoxin and on Early Cytokine Responses in a Murine In Vivo Models. Journal of Antimicrobial Chemotherapy. 2002; 50(1): 51-58.

Miettinen M, Veckman V, Latvala S, Sareneva T, Matikainen S, and Julkunen I. Live Lactobacillus Rhamnosusand Streptococcus Pyogenes Differentially Regulated Toll-Like Receptor (TLR) Gene Expression in Human Primary Macrophage. Journal of Leukocyte Biology. 2008; 84(4): 1092-1100.

Lu YC, Yeh WC, and Ohashi PS. LPS/TLR4 Signal Transduction Pathway. Cytokine. 2008; 42(2): 145-151.

Boele LC, Bajramovic JJ, Vries AMD, Voskamp-Visser IA, Kaman WE,and Kleij DVD. Activation of Toll Like Receptors and Dendritic Cells by a Broad Range of Bacterial Molecules. Cellular Immunology. 2009;255(1-2): 17-25.

Bhattacharyya S, Dudeja PK, and Tobacman JK. Lipopolysaccharide Activates NF-B By TLR4-Bcl10-Dependent And Independent Pathways In Colonic Epithelial Cells. American Journal of Phsiology-Gastrointestinal and Liver Physiology. 2008; 295: 784-790.

Gadjeva M, Tomczak MF, Zhang M, et al. A Role For NF-(Kappa) B Subunits P50 And P65 in the Inhibition of Lipopolisaccharide-Induced Shock. The Journal of Immunology. 2004; 173(9): 5786-5793.

Karrasch T and Jobin C. NF-kB and the Intestine: Fried or Foe? Inflammatory Bowel Diseases. 2008; 14(1): 114-124.

Yan F and Polk DB. Disrubtion of NF-kB Signaling by Ancient Microbial Molecules:Novel Therapies of the Future? Gut. 2010; 59(4): 421-426.

Putera AM, Endaryanto A, Athiyyah AF, Ranuh IRG, dan Sudarmo SM. Pengaruh Pemberian Probiotik terhadap Kesigapan Respon Innate Mukosa dalam Menghadapi Pajanan Pathogen. [Penelitian]. Surabaya, Universitas Airlangga. 2009.

Petrof EO, Kojima K, Ropeleski MJ, et al. Probiotics Inhibits Nuclear Factor-Kb And Induced Heat Shock Proteins In Colonic Epithelial Cells Through Proteasome Inhibition. Gastroenterology. 2004;127(5): 1474-1487.

Ruiz PA, Hoffmann M, Szcesny S, Blaut M, and Haller D. Innate Mechanism for Bifidobacterium Lactis to Activated Transient Pro-Inflammatory Host Responses in Intestinal Epithelial Cells after the Colonization of Germ-Free Rats. The Journal of Immunology. 2005; 115: 441-450.

Matsumoto S, Hara T, Hori T, et al. Probiotics Lactobacillus-Induced Improvement in Murine Chronic Inflammatory Bowel Disease is Associated with the Down-Regulation of Pro-Inflammatory Cytokines in Lamina Propia Mononuclear Cells. Clinical and Experimental Immunology. 2005; 140(3): 417-426.

Kim JM, Kim JS, Kim YJ, et al. Conjugated Linoleic Acids Produced by Lactobacillus Dissociated IKK-G and Hsp90 Complex in Helicobacter Pylori-Infected Gastric Epithelial Cells. Laboratory Investigation. 2008; 88(5): 541-552.

Okada Y, Tsuzuki Y, Hokari R, et al. Anti-Inflammatory Effects of the Genus Bifidobacterium on Macrophages by Modification of Phospho-Ikb and SOCS Gene Expression. International Journal of Experimental Pathology. 2009; 90(2): 131-140.

Sougioultzis S, Simeonidis S, Bhaskar KR, et al. Saccharomyces Boulardii Produces a Soluble Anti-inflammatory Factor that Inhibits NF-kB-mediated IL-8 Gene Expression. Biochemical and Biophysical Research Communications. 2006; 343(1): 69-76.

Couper K, Blount D, and Riley EM. IL 10: The Master Regulatory of Immunity to Infection. The Journal of Immunology. 2008; 180(9): 5771-5777.

Asihing T. Pengaruh Pemberian L. rhamnosus dan L.acidophilus terhadap Sekresi Sitokin Th1, Treg, Th17 pada Mukosa Usus Mencit yang Terpapar Lipolisakarida E.coli. [Penelitian]. Malang, Universitas Brawijaya. 2011.

Karin M, Cao Y, Greten FR, and Li ZW. NF-kB in Cancer: From Innocent by Stander to Major Culprit. Nature Reviews Cancer. 2002; 2(4): 301-310.

Conner JR, Smirnova II, Mosseman AP, and Poltorak A. IRAK1BP1 Inhibits Inflamation by Promoting Nuclear Translocation of NF-kB p50. Proceedings of the National Academy of Science of the United States of America. 2010; 107(25): 11477-11482.

Lawrence T, Gilroy DW, Colville-Nash PR, and Willoughby DA. Possible New Role for NF-kB in the Resolution of Inflammation. Nature Medicine. 2001; 7(12): 1291-1297.. Larsson R, Rocksen D, Liliehook B, Jonsson A, and Bucht A. Dose-dependent Activation of Lymphocytes in Endotoxin-Induced Airway Inflammation. Infection and Immunity. 2000; 68(12): 6962-6969.

Larsson R, Rocksen D, Liliehook B, Jonsson A, and Bucht A. Dose-dependent Activation of Lymphocytes in Endotoxin-Induced Airway Inflammation. Infection and Immunity. 2000; 68(12): 6962-6969.

Downloads

Issue

Vol. 26 No. 3 (2011)

Section

Research Article

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).

Most read articles by the same author(s)