Hubungan antara Stres Oksidatif dengan Kadar Hemoglobin pada Penderita Thalassemia/Hbe

Authors

  • Moedrik Tamam Departemen Ilmu Kesehatan Anak Fakultas Kedokteran Universitas Diponegoro/Rumah Sakit Umum Pusat Dr . Kariadi Semarang
  • Suharyo Hadisaputro Departemen Ilmu Penyakit Dalam Fakultas Kedokteran Universitas Diponegoro/Rumah Sakit Umum Pusat Dr . Kariadi Semarang
  • Sutaryo Sutaryo Departemen Ilmu Kesehatan Anak Fakultas Kedokteran Universitas Gajah Mada/Rumah Sakit Umum Pusat Dr . Sardjito Yogyakarta
  • Iswari Setianingsih Lembaga Biologi Molekuler Eijkman Jakarta
  • Djokomoeljanto Djokomoeljanto Departemen Ilmu Penyakit Dalam Fakultas Kedokteran Universitas Diponegoro/Rumah Sakit Umum Pusat Dr . Kariadi Semarang
  • Ag Soemantri Departemen Ilmu Kesehatan Anak Fakultas Kedokteran Universitas Diponegoro/Rumah Sakit Umum Pusat Dr . Kariadi Semarang

DOI:

https://doi.org/10.21776/ub.jkb.2012.027.01.7

Abstract

Pemberian  transfusi  darah  kronik  pada  penderita  thalassemia  dapat   menyebabkan  kelebihan  kadar  besi.  Kelebihan  kadar besi  bebas  dalam  tubuh  akan  memacu  timbulnya  oksidan  berupa  reactive  oxygen  species   (ROS)  yang  diukur  dalam  bentuk malondialdehide  (MDA).  Peningkatan  produksi  ROS  dapat  menyebabkan  kerusakan  membran  sel  yang  mengandung senyawa lipid    termasuk eritrosit. T ujuan penelitian ini  adalah untuk menilai hubungan antara kadar MDA serum dengan kadar Hb penderita thalassemia. Metode penelitian ini  adalah penelitian observasional kohort prospective dilaksanakan di  UTD  PMI  Kota  Semarang.  Selama  bulan  Januari  2006  sampai  dengan  Desember  2009  dijumpai  32  penderita  thalassemia/HbE.  Variabel  penelitian kadar Hb dan MDA   serum.  Pengukuran dilakukan sebelum  transfusi  ke-1, setelah transfusi  ke-1 dan sebelum transfusi  ke-2. Analisis statistik menggunakan uji Mann-Whitney,  uji Wilcoxon  dan uji korelasi Spearman. Rerata  umur  subjek  penelitian  adalah  9,5±  3,13  tahun,  laki-laki  adalah  25  orang  (46,9%)  dan  perempuan  17  orang  (53,1%). Status  gizi  43,8  %  termasuk  gizi  kurang.  Kadar  MDA   kelompok   Hb  sebelum  transfusi  ke-2  8  g/dL  2,89±0,451  dan  kelompok Hb  >8  g/dL  2,19±0,792  (p=0,01).  Ada  korelasi  negatif  antara  Hb  sebelum  transfusi  ke-2  dengan  MDA  (r=min  0,52;  p=0,002). Dapat  disimpulkan  ada  korelasi  negatif  antara  kadar  MDA  serum  dengan  kadar  Hb  pada  penderita  thalassemia  /HbE. Kata  Kunci:  Hemoglobin,  MDA,  stres  oksidatif ,  thalassemia  /HbE

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Published

2013-04-25

Issue

Vol. 27 No. 1 (2012)

Section

Research Article

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