EFEK HAMBATAN EKSTRAK DAUN CEPLUKAN (Physalis minima L)TERHADAP KONTRAKTILITAS OTOT POLOS USUS HALUS TERPISAH MARMUT DENGAN STIMULASI METAKOLIN EKSOGEN

Abstract

Physalis sp. has lots of merits as a kind of traditional medicine and one of them is its function as an alternatif treatment for diarrhea. Physalis minima leaf contains alkoloid atropine which is assumed can inhibit the contractionof guinea pig’s isolated ileum smooth muscle. This research was to know the effect of Physalis minima leaf extract toward the contraction of small intestine smooth muscle. This research was  an experimental study using “The Post Control GroupDesignâ€, and guinea pig’s isolated ileum as samples. The samples  consisted of five guinea pigs, each sample was given four kinds of treatment. The control was given metacholine at the  dose of 10 -5 without Physalis minima leaf extract. Before givingmetacholine at the dose of 10 -5 , the samples were given the first dosage of Physalis minima leaf extract (0.15%), the second dose (0.30%) and the third dose (0.45%) so getting four graphs for each sample. The level of contraction was recorded by using kymograph. The result showed that  the ileum contraction of the third group was inhibited (One Way ANOVA, p=0.041), and there was a relation between the increasing dosage of Physalis minima leaf extract and the decreasing ileum contraction (simple linear regression, with R=-0.608 p=0.04 and regression equation y=52.150 – 7.660x).