Efek Penghambatan Ekstrak Akuades Daun Ocimum basilicum terhadap Streptococcus mutans In Vitro
DOI:
https://doi.org/10.21776/ub.jkb.2018.030.02.2Keywords:
Ekstrak akuades, Ocimum basilicum, spektofotometri, Streptococcus mutansAbstract
Daun kemangi (Ocimum basilicum) telah diteliti ekstraknya sebagai antibakteri patogen pangan. Penelitian eksperimental antibakteri dari ekstrak daun Ocimum basilicum terhadap patogen di rongga mulut, termasuk Streptococcus mutans, masih harus diperdalam. Penelitian ini bertujuan untuk mengetahui efek ekstrak daun Ocimum basilicum terhadap penghambatan pertumbuhan Streptococcus mutans. Daun Ocimum basilicum diekstraksi dengan metode maserasi menggunakan akuades steril. Ekstrak diuji penghambatannya terhadap pertumbuhan Streptococcus mutans dalam larutan NaCl fisiologis dengan metode dilusi dan spektrofotometri 550nm. Hasil penelitian menunjukkan terdapat pengaruh yang signifikan perlakuan ekstrak daun Ocimum basilicum terhadap penghambatan pertumbuhan Streptococcus mutans (p<0,05). Ekstrak daun kemangi (Ocimum basilicum L.) berpengaruh menghambat pertumbuhan Streptococcus mutans.Â
Downloads
References
Sirait N. Penggunaan Berbagai Jenis Tanaman Obat untuk Menanggulangi Bau Badan. Warta Penelitian dan Pengembangan Tanaman Industri. 2008; 14(3): 8-11.
Pushpangadan P and George V. Basil. In: Peter KV (Ed.). Handbook of Herbs and Spices. Volume 1. Second Edition. Cambridge: Woodhead Publishing; 2012; p. 55-72.
Bilal A, Jahan N, Ahmed A, Bilal SN, Habib S, and Hajra S. Phytochemical and Pharmacological Studies on Ocimum Basilicum Linn-A Review. International Journal of Current Research and Review. 2012; 4 (23): 73-83.
Ahonkhai I, Ba A, Edogun O, and Mu U. Antimicrobial Activities of the Volatile Oils of Ocimum Basilicum L. and Ocimum Gratissimum L. (Lamiaceae) against Some Aerobic Dental Isolates. Pakistan Journal of Pharmaceutical Sciences. 2009; 22(4): 405-409.
Dhotre S, Suryawanshi N, Selkar S, and Nagoba B. Viridans Group Streptococci and the Oral Ecosystem. European Journal of General Medicine. 2015; 13(2): 145-148.
Fatmawati DWA. Hubungan Biofilm Streptococcus Mutans terhadap Resiko Terjadinya Karies Gigi. Stomatognatic. 2011; 8(3): 127-130.
Parhusip AJN, Wijaya JR, dan Stenlie J. Aktivitas Antimikroba Ekstrak Daun Kemangi (Ocimum Basilicum L.) terhadap Mikroba Patogen Pangan. Jurnal Ilmu dan Teknologi Pangan. 2009; 7(2): 77-92.
Santosaningsih D, Zuhriyah L, and Nurani M. Staphylococcus Aureus pada Komunitas Lebih Resisten terhadap Ampisilin dibandingkan Isolat Rumah Sakit. Jurnal Kedokteran Brawijaya. 2011; 26(4): 204-207.
Sutton S. Measurement of Microbial Cells by Optical Density. Journal of Validation Technology. 2011; 17(1): 46-49.
Tortora GJ, Funke BR, and Case CL. Microbiology: An Introduction. 10th edition. San Francisco: Pearson Benjamin Cummings; 2010; p. 179, 301, 318.
Mishra P and Mishra S. Study of Antibacterial Activity of Ocimum Sanctum Extract against Gram Positive and Gram Negative Bacteria. American Journal of Food Technology. 2011; 6(4): 336-341.
Kaya I, YiÄŸit N, and Benli M. Antimicrobial Activity of Various Extracts of Ocimum Basilicum L. and Observation of the Inhibition Effect on Bacterial Cells by Use of Scanning Electron Microscopy. African Journal of Traditional, Complementary, and Alternative Medicines. 2008; 5(4): 363-369.
Hasan S, Danishuddin M, Adil M, Singh K, Verma PK, and Khan AU. Efficacy of Emblica officinalis on the Cariogenic Properties of Streptococcus Mutans: A Novel and Alternative Approach to Suppress Quorum-Sensing Mechanism. PLoS ONE. 2012; 7(7): 1-12.
Edriss AE, Alabjar ZA, and Satti AA. Phytochemical Screening of Important Secondary Metabolites in Some Extracts of Two Sudanese Plants. Global Advanced Research Journal of Environmental Science and Toxicology. 2012; 1(8): 199-202.
Ferrazzano GF, Amato I, Ingenito A, Zarrelli A, Pinto G, and Pollio A. Plant Polyphenols and Their Anti-Cariogenic Properties: A Review. Molecules. 2011; 16(2): 1486-1507.
Yahya NZ. In Vitro and in Vivo Evaluation of Antimicrobial Effect of Leaves Ocimum Basilicum of Ethanolic Extract against Staphylococcus Aureus. Al-Anbar Journal of Veterinary Sciences. 2011; 4(2): 67-75.
Cushnie TPT and Lamb AJ. Recent Advances in Understanding the Antibacterial Properties of Flavonoids. International Journal of Antimicrobial Agents. 2011; 38(2): 99-107.
Fitrianti D, Noorhamdani AS, dan Karyono SS. Efektivitas Ekstrak Daun Ceplukan sebagai Antimikroba terhadap Methicillin-Resistant Staphylococcus Aureus in Vitro. Jurnal Kedokteran Brawijaya. 2011; 26(4): 212-215.
Fani M and Kohanteb J. Inhibitory Activity of Aloe Vera Gel on Some Clinically Isolated Cariogenic and Periodontopathic Bacteria. Journal of Oral Science. 2012; 54(1): 15-21.
Berniyanti T and Mahmiyah E. Microbiological Studies on the Production of Antimicrobial Agent by Saponin Aloe Vera Linn against Streptococcus Sanguinis. Research Journal of Microbiology. 2015; 10(8): 486-493.
Singh V, Kuldeep, Bajaj A, and Kalra M. Green Tea: Enhancing Oral Health. University Journal of Dental Sciences. 2016; 2(1): 61-63.
Peng L, Kang S, Yin K, et al. Antibacterial Activity and Mechanism of Berberine against Streptococcus Agalactiae. International Journal of Clinical and Experimental Pathology. 2015; 8(5): 5217-5223.
Published
Issue
Section
License
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
