HUBUNGAN ANTARA PENINGKATAN KADAR LEKOTRIEN B4 (LTB-4) DENGAN HIPOSELULARITAS SUMSUM TULANG (PENELITIAN PADA ASPIRAT SUMSUM TULANG)
DOI:
https://doi.org/10.21776/ub.jkb.2005.021.02.2Abstract
ABSTRACT Previous study on human bone marrow culture showed that leukotrien B-4 (LTB-4) which was added to granulocyte-macrophage and erytroid colony, suppressed the growth of colonies. This study assessed the correlation between LTB-4 and marrow cellular. Marrow aspirate which was taken from 60 adult patients (26 men and 34 women) suffering from various haematopoietic problems was examined for amount of LTB-4 and the degree of marrow cellular. ELISA principle was applied for LTB-4 measurement and marrow cellular assessed by counting the member of nucleated cells per cubic-mm of marrow aspirate using microscopic counting chamber principle. Existing difference of the strength of correlation of LTB-4  and  marrow  cellular  was  analized  using  manova and  path  analysis  and  expressed  as  path  coefficient.The difference of confounding factors such as gender, age and type of diseases (haematopoitic problems) were controlled and homogenized during statistical analysis using SPSS 10 program. Path coefficient in the multi cellular marrow (n = 60) was – 0.092 (p = 0.495) and path coefficient in hypo cellular marrow (n = 28) was – 0.406 (p = 0.013). This study concludes that the increase of amount of LTB-4 in hypo cellular marrow is associated with the decrease of marrow cellular. Keywords : Leukotrien B-4, Hypocelular marraw, Apoptosis, HematopoisisDownloads
References
DAFTAR KEPUSTAKAAN
Estrove Z, Halperin DS, Coceani F, Freedman MH. Modulation of Human Marrow Haematopoiesis by Leukotrien in
Vitro. British Journal of Haematology 1988;69:321–327.
Claesson HE, Dahlberg N, Garton G. Stimulation of Human Myelopoiesis by Leukotrien B-4. Biochemical and Biophysical Research Communication 1985;131:579–585.
Handersson WR. The Role of Leukotrien in Inflammation. Ann Intern Med 1994;121:684–697.
Sigal LH, Ron Y. Immunology and Inflammation, Basic Mechanism and Clinical Consequences. New York: McGraw-Hill
Inc.;1994;271–286.
Abbas AK, Lichtman AH, Prober JS. Cellular and Molecullar Immunology, 4th edition. Philadelphia: WB Saunders Co.;
;424-444.
Jackobson PJ, Odlander B, Steinhiller D, Rosen A, Claesson HE. Biochemical and Biophysical Research Communication. 1991;178:302–308.
Den Ottolander GJ. The Bone Marrow Asprate of Healthy Subjects. British Journal of Haematology 1996;95:570–576.
Widjajanto E, Budiman. Mast Cells Hyperactivity May Suppress Haematopoiesis in Aplastic Marrow (anemia aplastic):a preliminary study. International Journal on Hematology 1996;64(Suppl.1).S28.
Dacie JV, Lewis SM. Practical Haematology. 5th edition. London: The ELBS and Churchil Livingstone;1975;150–168.
Dillon WR, Goldstein M. Multivariate Analysis, Method and Aplication. New York: Jhon Wiley & Sons; 1987; 430-486.
Church MK. Human mast cell development, heterogeneity, and cytokines. In (Oeheling AK, Lopez JGH, eds). Progress
in Allergy and Clinical Immunology Volume 4, Cancun Mexico), October 19 – 24, 1997. Seatle: Hogfere & Huber Publishers;1997;95–98.
Dalton R, Chan L, Batten E, Eridani S. Mast Cell Leukemia: Evidence for Bone Marrow Origin of Pathological Clone. British Journal of Haematthology 1986;64:397–406.
Melman SA. Mast Cells and Their Mediators, Emphasis on Their Role in Type i Immediate Hypersensitivity in Canines.
International Journal of Dermatology 1987;26:335-344.
Young NS, Maciejewski J. The Pathophysiology of Acquired Aplastic Anaemia. The New England Journal of Medicine 1997;336:1365–1372.
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