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Analisis Konsentrasi Low Density Lipoprotein Teroksidasi Serum pada Tahapan Aterosklerosis

ismawati ismawati, fadil oenzil, yanwirasti yanwirasti, eti yerizel
  Jurnal Kedokteran Brawijaya, Vol 29, No. 4 (2017),    

Abstract


Aterosklerosis merupakan proses yang terjadi secara bertahap meliputi tahap inisiasi, progresi dan komplikasi. Beberapa penelitian memperlihatkan kaitan antara stres oksidatif dengan aterosklerosis. Tetapi belum ada penelitian yang menganalisis perbedaan stres oksidatif pada berbagai tahap aterosklerosis. Konsentrasi Oxidized Low Density Lipoprotein (oxLDL)  menggambarkan stres oksidatif yang terjadi sehingga dikenal sebagai marker stres oksidatif. Penelitian ini bertujuan  untuk menganalisis konsentrasi oxLDL serum pada berbagai tahap aterosklerosis pada hewan coba. Pada penelitian ini dilakukan pengukuran kadar oxLDL serum pada  24 ekor tikus yang terdiri dari  kelompok normal, inisiasi, progresi dan komplikasi. Induksi aterosklerosis dilakukan  dengan pemberian vitamin D3 (700.000 IU/kg) dan  pakan tinggi lipid (kolesterol 2%, lemak kambing 5%, asam kolat 0,2%) masing-masing selama 2 hari, 4 hari dan 2 minggu. Konsentrasi oxLDL   serum diukur   dengan teknik Enzyme linked immunosorbent assays (ELISA). Data disajikan sebagai rata-rata±SD. Uji statistik yang digunakan adalah uji Anova. Nilai p<0,05 dikatakan bermakna secara statistik. Konsentrasi oxLDL serum lebih tinggi pada inisiasi dibandingkan normal (2,17±0,26 ng/mL versus 1,93±0,25 ng/mL, p>0,05) dan lebih tinggi lagi pada progresi dan komplikasi (2,75±0,25 ng/mL dan 3,3±0,61 ng/mL, p < 0,05 masing-masing terhadap normal dan inisiasi). Penelitian ini menyimpulkan bahwa terdapat perbedaan konsentrasi oxLDL pada berbagai tahap aterosklerosis dan konsentrasi oxLDL serum  paling tinggi terdapat pada tahap komplikasi. Hal ini membuka kemungkinan untuk mengembangkan oxLDL sebagai marker tahapan aterosklerosis.


Keywords


aterosklerosis, oxLDL, stres oksidatif

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