Pengembangan Model Mencit Alergi dengan Paparan Kronik Ovalbumin
DOI:
https://doi.org/10.21776/ub.jkb.2009.025.01.1Abstract
ABSTRACTHistology expression in asthma show inflammatory processes and airway structural changes. Treatment
development in asthma needs appropriate animal models but most animal models use short term allergen
exposure. The aim of this research was to create develop allergic mouse model with allergic inflammation
and structural changes histology expression. Eight female BALB/c mice, 6-12 weeks old, 80-100 grams
were divided into two groups. The first group were exposed to a normal saline solution where the second
group received ovalbumin. Mice were sensitized by intraperitoneal injection of ovalbumin on day 0 and 14
and challenged by nebulization of 1% ovalbumin three times weekly for 6 weeks. Twenty four hours after
final exposure, the mice were sacrificed by lethal cardiac puncture. The level of total IgE in serum were
measured by ELISA and IL-4 receptor expression were examinated by immunohistochemistry.
Morphometric analysis was performed to count inflammatory cells, eosinophil, goblet cells, epithel and
smooth muscle thickness. Differences between the control and exposed mice were analyzed by Mann-
Whitney test. There were a significant differences in total IgE serum and expression IL-4 receptor between
the two groups (P < 0,05). The morphometric analyzing also demonstrated significant differences between
these two groups (P < 0,05). Chronic ovalbumin exposure through inhalation produced allergic
inflammation and airway structural changes.
Keywords: allergic inflammation, mouse models, chronic exposure, ovalbumin
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DAFTAR KEPUSTAKAAN
Bousquet J, Jeffery PK, Busse WW, Johnson M, Vignola AM. Asthma: from bronchoconstrictions to airways inflammation and remodeling. Am J Respir Crit Care Med 2000; 161:1720-45
Tang MLK, Wilson JW, Stewart AG, Royce SG. Airway remodelling in asthma: Current understanding and implications for future therapies . Pharmacology & Therapeutics. 2006; 112:474488
Redington AE. Fibrosis and airway remodeling. Clin Exp Allergy 2000; 30:42-5
Shinagawa K, Kojima M. Mouse model of airway remodeling: Strain differences . Am J Repir Crit
Care Med. 2003; 168:959-67
Temelkovski J, Hogan SP, Shepherd DP, Foster PS, Kumar R. An improved murine model of asthma: selective airway inflammation, epithelial lesions and increased methacholine responsiveness following chronic exposure to aerosolised allergen . Thorax. 1998; 53:849-56
Locke NR, Royce SG, Wainewright JS, Samuel CS, Tang ML. Comparison of airway remodeling in acute, subacute, and chronic models of allergic airways disease . . Am J Respir Cell Mol Biol 2007; 36:625-32
Akers IA, Parsons M, Hill MR, Hollenberg MD, Sanjar S, Laurent GJ, . et al Mast cell tryptase stimulates human lung fibroblast proliferation via protease-activated receptor-2 Am J Physiol Lung Cell Mol Physiol. 2000;278: 193 201
Phipps S, Benyahia F, Ou TT, Barkans J, Robinson DS, Kay AB. Acute allergen-induced airway remodeling in atopic asthma . Am J Respir Cell Mol Biol 2004;31: 626 32.
Holgate S, Lackie P, Davies DE, Roche RW, Walls AF. The bronchial epithelium as a key regulator of airway inflammation and remodeling in asthma. Clin Exp Allergy .1999; 29: 90.
Sheppard D. Airway epithelial integrins: why so many? Am J Respir Cell Mol Biol -1998; 19:349 351.
Knight D. Epithelium-fibroblast interactions in response to airway inflammation. Immunol Cell Biol . 2001; 160 164.
Minshall EM, Chakir J, Laviolette M, Molet S, Zhu Z, Olivenstein R, et al. IL-11 expression is increased in severe asthma: association with epithelial cells and eosinophils. J Allergy Clin Immunol 2000;105: 232-38.
Fedorov I, Wilson S, Davies D, Holgate S. Epithelial stress and structural remodelling in childhood asthma. Thorax 2005;60:389 394.
Ebina M, Takahashi T, Chiba T, Motomiya M. Cellular hypertrophy and hyperplasia of airway smooth muscles underlying bronchial asthma. A 3-D morphometric study (abstract). Am Rev Respir Dis 1993; 148: 720 726.
Benayoun L, Druilhe A, Dombret MC, Aubier M, Pretolani M. Airway structural alterations selectively associated with severe asthma. Am J Respir Crit Care Med 2003;167: 1360 1368.
Woodruff PG, Dolganov GM, Ferrando RE, Donnelly S, Hays SR, Solberg OD, et al. Hyperplasia of smooth muscle in mild to moderate asthma without changes in cell size or gene expression . Am J Respir Crit Care Med. 2004; 169: 1001 1006.
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