Outer Membrane Protein 49,4 Kda dari Porphyromonas gingivalis Merupakan Protein Hemaglutinin dan Adhesin Terhadap Netrofil
DOI:
https://doi.org/10.21776/ub.jkb.2009.025.02.6Abstract
ABSTRACTPeriodontitis is caused mostly by P. gingivalis and it is related to acute coronary syndrome. P. gingivalis readily
invades into blood circulation and potentially induces. Collagenolytic activity of neutrophil which result in
collagen vascular degradation lead to atherosclerotic plague rupture (APR). APR is responsible for occurring
fatal cardiovascular events such as acute myocardial infarction (AMI). These information brought out notion
concerning the adhesion interaction of P. gingivalis with neutrophil. The aim of this study was to assess
adhesion molecule of P. gingivalis outer membrane protein (OMP) by partial characterization took in
hemagglutination assay using mice erythrocytes, adhesion inhibition assay by gradual concentration of
adhesion blocked in neutrophil, immunologic assay using Western-blotting and immunocytochemistry. The
results showed that 49,4 kDa P.gingivalis OMP can agglutinate mice erythrocytes and adherence to netrophil
Increase concentration of OMP P. gingivalis 49,4 kDa reduce adhesion process to netrophil This protein was
recognized by the polyclonal antibody of 49,4 kDa adhesion molecule. It can be concluded that P gingivalis
outer membrane protein is a hemagglutinin and an adhesion molecule to netrophil
Keywords : 49,4 kDa P. gingivalis OMP, neutrophil, hemagglutination, adhesin
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