Pengaruh Amitriptyline Dosis Lethal pada Bangkai Tikus Rattus Norvegicus strain Wistar terhadap Pertumbuhan Larva Musca Sp.
DOI:
https://doi.org/10.21776/ub.jkb.2010.026.02.6Abstract
ABSTRAKKata Kunci:Â Amitriptyline, Musca sp., pertumbuhan larva
Peran Post Mortem Interval (PMI) sangat penting untuk memperkirakan waktu kematian yang dapat diketahui dengan
menentukan umur larva lalat yang terdapat pada jenazah. Pertumbuhan larva lalat sangat dipengaruhi oleh suhu
lingkungan, makanan, kelembapan, intensitas cahaya, dan kontaminan. Keberadaan obat antidepresan dapat
mengacaukan estimasi waktu kematian hingga 77 jam. Penelitian ini merupakan penelitian eksperimental menggunakan
media dua bangkai tikus. Tikus pertama dibunuh dengan cara diberikan amitriptyline 100 mg peroral (LD50 = 350
mg/kgBB).  Tikus kedua dibunuh dengan cara dislokasi cervical. Kedua bangkai ini dimasukkan ke 2 kandang yang telah
berisi masing-masing 50 lalat Musca sp. Dilakukan pengamatan setiap 12 jam mulai dari larva lalat stadium satu sampai
lalat dewasa. Setiap media tumbuh diambil 10 larva secara acak setiap hari untuk diukur panjang dan berat serta durasi
pertumbuhannya. Hasil penelitian menunjukkan bahwa pertumbuhan larva pada media dengan kandungan amitriptyline
berbeda secara signifikan dibandingkan pertumbuhan larva pada media tanpa kandungan amitriptyline. Panjang larva
pada media tumbuh dengan kandungan amitriptyline lebih panjang, berat larva pada media tumbuh dengan kandungan
amitriptyline lebih berat dan durasi pencapaian stadium lebih lambat sejak hari ke-3a (p < 0,005). Hasil penelitian ini
menunjukkan bahwa amitriptyline dapat memperlambat pertumbuhan larva lalat Musca sp.
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