Polimorfisme Gen CYP1A1 (3801 T/C dan Ile462Val) pada Pasien Kanker Serviks

Swandari Paramita, Soetomo Soewarto, Mohammad Aris, Sutiman Bambang

Abstract


ABSTRAK

Kanker serviks menempati peringkat pertama kanker di Indonesia dengan faktor risiko merokok, paritas tinggi dan penggunaan kontrasepsi hormonal. Sitokrom P450 1A1 (CYP1A1) memegang peran dalam metabolism karsinogen pada kanker servik yaitu Benzo[a]pyrene dan estrogen. Tujuan penelitian ini adalah untuk mengidentifikasi frekuensi dan distribusi polimosfisme gen CYP1A1 (3801 T/C and LLe462Val) pada kanker servik. Polimorfirme gen CYP1A1 dianalisis dengan menggunakan metode PCR-RFLP. Pada polimorfisme gen CYP1A1 (3801TC) ditemukan 36.1% dengan tipe wild-types T/T, 37.9% heterozigot T/C dan 25.9% homozigot C/C. Pada polimorfisme gen CYP1A1 (Ile 462Val) didapatkan 56.9% tipe wild-types Ile/Ile, 37.9% heterozigot Ile/Val dan 5.2% homozigot Val/Val. Gambaran polimorfisme tersebut sama dengan penelitian lain di Asia.

Kata Kunci:  CYP1A1 gene polymorphisms, kanker servik


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